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[190917_at]

Description C53B4.7 /REP_DB=WormBase Gene ID /WP=CE23608 /CHR=4 /FEA=Sanger Annotation /DEF=GDP-D-mannose dehydratase (HINXTON) SW:Q18801 protein_id:CAA92455.2
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 71      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 53      6grp_total > 81      4grp_total > 57      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 769
1 2 3 4 range
A 558.5
(-2.4)
P 		1034.9
(3)
P 		1033.1
(3)
P 		505.5
(-3)
P 		529
B 551.5
(-2.2)
P 		899.5
(1.7)
P 		1012.2
(3)
P 		483.6
(-3)
P 		529
C 648.9
(-1.6)
P 		939.3
(0.7)
P 		1238.3
(3)
P 		469.6
(-3)
P 		769
avr 586.3
(-2) 		957.9
(1.7) 		1094.5
(3) 		486.2
(-3) 		608

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1031
1 2 3 4 5 6 range
A 574
(-2.7)
P 		744
(-1.7)
P 		799.8
(-1.3)
P 		1543.1
(3)
P 		787
(-1.4)
P 		514.6
(-3)
P 		1029
B 722.6
(-0.4)
P 		705.9
(-0.6)
P 		861.8
(1.3)
P 		1003.2
(3)
P 		976
(2.7)
P 		512.4
(-3)
P 		491
C 933.9
(0.4)
P 		752.9
(-1.5)
P 		929.9
(0.3)
P 		1193.6
(3)
P 		1084
(1.9)
P 		603.2
(-3)
P 		590
avr 743.5
(-1.3) 		734.3
(-1.4) 		863.8
(-0.3) 		1246.6
(3) 		949
(0.5) 		543.4
(-3) 		703
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 358
1 2 3 4 range
A 203.4
(1)
P 		224.7
(1.6)
P 		270.1
(3)
P 		74
(-3)
P 		196
B 175.9
(0.3)
P 		222.2
(2.4)
P 		234.5
(3)
P 		103.1
(-3)
P 		131
C 214.8
(-0.7)
P 		270.1
(0.3)
P 		431.6
(3)
P 		79.2
(-3)
P 		352
avr 198
(-0) 		239
(1.1) 		312.1
(3) 		85.4
(-3) 		227

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 105.8
(-3)
P 		264.6
(0.3)
P 		174.2
(-1.6)
P 		391.4
(3)
P 		237.5
(-0.2)
P 		125.3
(-2.6)
P 		286
B 251.4
(-1.2)
P 		235
(-1.7)
P 		209.3
(-2.5)
P 		265.7
(-0.8)
P 		396.6
(3)
P 		191.1
(-3)
P 		206
C 346.1
(2.8)
P 		229.7
(-1.9)
P 		236.7
(-1.6)
P 		240
(-1.5)
P 		350.8
(3)
P 		201.9
(-3)
P 		149
avr 234.4
(-0.6) 		243.1
(-0.3) 		206.7
(-1.7) 		299
(1.9) 		328.3
(3) 		172.8
(-3) 		156
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190917_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8536
Sequence Type Exemplar sequence
Representative Public ID C53B4.7 NCBI
Target Description C53B4.7 /REP_DB=WormBase Gene ID /WP=CE23608 /CHR=4 /FEA=Sanger Annotation /DEF=GDP-D-mannose dehydratase (HINXTON) SW:Q18801 protein_id:CAA92455.2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069162(11), NM_069163(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069162 NCBI Caenorhabditis elegans C53B4.7a (C53B4.7) mRNA, complete cds. 11/11 None
NM_069163 NCBI Caenorhabditis elegans C53B4.7b (C53B4.7) mRNA, complete cds. 11/11 None
SNAP00000003710 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8987968:8990254:1 11/11 None
GENEFINDER00000003716 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8987968:8990254:1 11/11 None
C53B4.7a ENSEMBL cdna:known chromosome:CEL140:IV:8987248:8990406:1 gene:C53B4.7 11/11 None
C53B4.7b ENSEMBL cdna:known chromosome:CEL140:IV:8987935:8990254:1 gene:C53B4.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8988776-8990260(+) 100.0 100.0
Public Domain and Genome References
Ensembl C53B4.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC42270 Hypothetical protein C53B4.7a [Caenorhabditis elegans] ref|NP_501563.1| C53B4.7a [Caenorhabditis elegans] 0.0
blast CAC42269 Hypothetical protein C53B4.7b [Caenorhabditis elegans] ref|NP_501564.1| C53B4.7b [Caenorhabditis elegans] sp|Q18801|GMD1_CAEEL Probable GDP-mannose 4,6 dehydratase 1 (GDP-D-mannose dehydratase) (GMD) 0.0
blast CAE74082 Hypothetical protein CBG21737 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:190917_AT
agactccattctatccaagatctccatatgctgttgccaagatgtacgggtactggattg
ttgttaactatcgtgaagcctacaatatgtttgcttgcaatggaattttgttcaaccatg
agagtccaagaagaggagagacattcgtgactcgtaagatcactcgttcagtcgctaaaa
tcagtttggggcaacaggaaagcattgagctcggaaatctgagtgctcttcgtgattggg
gacacgccagagagtatgttgaagcaatgtggagaattcttcaacacgactcaccagatg
attttgtgattgcaactggaaaacaattcagtgtcagagagttttgtaatcttgcatttg
ctgaaattggagaagtattgcaatgggaaggagaaggtgttgaggaagttggaaagaata
aagatggagttattcgagttaaagttagtccaaagtactacagaccaactgaagtggaga
ctttattgggaaatgctgaaaaagcaaagaagactcttggatgggaagcaaaggttacgg
ttccggaactcgtcaaagag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACTCCATTCTATCCAAGATCTCC 611 71 512 Antisense
CAAGATCTCCATATGCTGTTGCCAA 210 185 527 Antisense
CTGTTGCCAAGATGTACGGGTACTG 637 3 542 Antisense
TGTTGTTAACTATCGTGAAGCCTAC 556 559 570 Antisense
GAGGAGAGACATTCGTGACTCGTAA 625 403 644 Antisense
GTAAGATCACTCGTTCAGTCGCTAA 257 463 665 Antisense
AGCTCGGAAATCTGAGTGCTCTTCG 577 79 719 Antisense
GTGATTGGGGACACGCCAGAGAGTA 675 481 743 Antisense
GAGAATTCTTCAACACGACTCACCA 344 391 783 Antisense
GAGTTTTGTAATCTTGCATTTGCTG 248 399 850 Antisense
TACGGTTCCGGAACTCGTCAAAGAG 445 639 1047 Antisense
Sequence Source Affymetrix Proprietary Database