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[190899_at] glutathione S-transferase

Description F21H7.1 /REP_DB=WormBase Gene ID /WP=CE15874 /TR=O45387 /GB=CAB07589.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=glutathione-S-transferase
Cluster 6grp_total > 50      4grp_total > 60      6grp_cgc > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 135
1 2 3 4 range
A 168.6
(1.2)
P 		116.3
(-3)
A 		191.6
(3)
P 		150.9
(-0.2)
M 		75
B 129.6
(0)
P 		148.2
(1)
P 		188.4
(3)
P 		70.7
(-3)
A 		118
C 194.3
(2.2)
P 		177
(1)
M 		205.8
(3)
P 		117.8
(-3)
M 		88
avr 164.2
(0.7) 		147.2
(-0.5) 		195.3
(3) 		113.1
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 137
1 2 3 4 5 6 range
A 162.5
(2.2)
P 		89.8
(-3)
A 		174.3
(3)
P 		163
(2.2)
P 		133.6
(0.1)
P 		109.2
(-1.6)
A 		85
B 224.5
(3)
P 		140.6
(-0.7)
P 		182
(1.1)
P 		162.8
(0.3)
P 		130.5
(-1.1)
P 		87.6
(-3)
A 		137
C 172.3
(0.9)
P 		123
(-1.7)
P 		191.3
(1.9)
P 		211
(3)
P 		99.3
(-3)
A 		101.6
(-2.9)
A 		112
avr 186.4
(3) 		117.8
(-1.7) 		182.5
(2.7) 		178.9
(2.5) 		121.1
(-1.5) 		99.5
(-3) 		87
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 61.4
(3)
P 		25.2
(-2.5)
A 		50.1
(1.3)
P 		22.1
(-3)
M 		39
B 41.3
(2.5)
P 		36.6
(1.5)
P 		43.7
(3)
P 		15.1
(-3)
A 		29
C 64.3
(2.1)
P 		50.9
(0.6)
M 		71.7
(3)
P 		19.9
(-3)
M 		52
avr 55.7
(3) 		37.6
(0) 		55.2
(2.9) 		19
(-3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 30
(-1.6)
P 		31.9
(-0.8)
A 		38
(1.7)
P 		41.3
(3)
P 		40.3
(2.6)
P 		26.6
(-3)
A 		15
B 78.1
(3)
P 		46.8
(-1.1)
P 		44.2
(-1.5)
P 		43.1
(-1.6)
P 		53
(-0.3)
P 		32.7
(-3)
A 		45
C 63.9
(3)
P 		37.5
(-2)
P 		48.7
(0.1)
P 		42.4
(-1.1)
P 		32.1
(-3)
A 		34
(-2.6)
A 		32
avr 57.3
(3) 		38.7
(-1.3) 		43.6
(-0.1) 		42.3
(-0.4) 		41.8
(-0.5) 		31.1
(-3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190899_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13200
Sequence Type Exemplar sequence
Representative Public ID F21H7.1 NCBI
Target Description F21H7.1 /REP_DB=WormBase Gene ID /WP=CE15874 /TR=O45387 /GB=CAB07589.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=glutathione-S-transferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074694(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074694 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-22) (gst-22) mRNA, complete cds. 11/11 None
SNAP00000014722 ENSEMBL cdna:SNAP chromosome:CEL140:V:16233671:16234854:-1 11/11 None
F21H7.1 ENSEMBL cdna:known chromosome:CEL140:V:16233671:16234854:-1 gene:F21H7.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000014732 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16233670-16234854(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-22
Ensembl F21H7.1
Entrez Gene 180088 Entrez gene
SwissProt O45387 EMBL-EBI
WormBase CE15874 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07589 Hypothetical protein F21H7.1 [Caenorhabditis elegans] ref|NP_507095.1| Glutathione S-Transferase family member (gst-22) [Caenorhabditis elegans] 1.0E-123
blast AAO38568 Glutathione s-transferase protein 34 [Caenorhabditis elegans] ref|NP_741060.2| Glutathione S-Transferase family member (gst-34) [Caenorhabditis elegans] 5.0E-95
blast AAM19064 Glutathione s-transferase protein 35 [Caenorhabditis elegans] ref|NP_741061.2| Glutathione S-Transferase family member (gst-35) [Caenorhabditis elegans] 3.0E-83
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 6.5E-4
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 4.2E-11
Sequence
Target Sequence 
>C. ELEGANS:190899_AT
tgaggatgttcgactgccgacgatcaatgctgttccgggatttcaaagtgacgaattttc
agcgttgaaagacaagaccccatttggccgattcacagttctttccatcgacggttttga
tcttgctcaatcttctgcaattcatcgttatctggctagaaagtttggctacgctggaaa
aacaccggaagacgaggcttttgctgattctatcgttgatcagtttaatgagtactacgc
gtcactccatccctaccgtgatgccgtgaaaactggaaaaacaatggaagaagtgaagca
aattcatgacaaagtttacattccagcaaaaaacttaatgttcaaaatattgactcgaat
tcttaaagagaacaaatcgggtttccttgttggagatggattgacttgggcagatctggt
ggtggctgatcatttgtatactctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAGGATGTTCGACTGCCGACGATC 289 567 99 Antisense
GCCGACGATCAATGCTGTTCCGGGA 578 283 114 Antisense
AAAGACAAGACCCCATTTGGCCGAT 488 111 166 Antisense
ATTTGGCCGATTCACAGTTCTTTCC 121 19 180 Antisense
CAGTTCTTTCCATCGACGGTTTTGA 668 205 194 Antisense
ACGGTTTTGATCTTGCTCAATCTTC 119 97 209 Antisense
CTGCAATTCATCGTTATCTGGCTAG 490 237 233 Antisense
GGAAGACGAGGCTTTTGCTGATTCT 436 531 285 Antisense
TAATGAGTACTACGCGTCACTCCAT 468 635 324 Antisense
CATCCCTACCGTGATGCCGTGAAAA 112 211 346 Antisense
GGTGGCTGATCATTTGTATACTCTG 387 499 519 Antisense
Sequence Source Affymetrix Proprietary Database