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[190892_at] ADP-Ribosyltransferase

Description E02H1.4 /REP_DB=WormBase Gene ID /WP=CE01539 /TR=SW:Q09525 /GB=CAA87379.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=ADP-Ribosyltransferase
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 86      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 94      6grp_total > 77      4grp_total > 47      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 238
1 2 3 4 range
A 539.7
(3)
P 		348
(-2.1)
P 		323.1
(-2.7)
P 		312.3
(-3)
P 		227
B 550.3
(3)
P 		399.8
(-2.4)
P 		381.9
(-3)
P 		429.7
(-1.3)
P 		168
C 531.5
(3)
P 		362.1
(-3)
P 		397.4
(-1.7)
P 		424.2
(-0.8)
P 		169
avr 540.5
(3) 		370
(-2.9) 		367.5
(-3) 		388.7
(-2.3) 		173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 442
1 2 3 4 5 6 range
A 450.6
(-3)
P 		485.9
(-2.5)
P 		482
(-2.5)
P 		536.8
(-1.7)
P 		559.2
(-1.3)
P 		841.1
(3)
P 		391
B 465.5
(-2.7)
P 		451.7
(-3)
P 		507.9
(-1.8)
P 		489.5
(-2.2)
P 		594.4
(-0)
P 		740.2
(3)
P 		289
C 411.7
(-3)
P 		449.3
(-2.5)
P 		460.3
(-2.3)
P 		555.9
(-1)
P 		483.5
(-2)
P 		853.8
(3)
P 		442
avr 442.6
(-3) 		462.3
(-2.7) 		483.4
(-2.3) 		527.4
(-1.6) 		545.7
(-1.3) 		811.7
(3) 		369
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 196.5
(3)
P 		75.5
(-1.8)
P 		84.5
(-1.5)
P 		45.7
(-3)
P 		151
B 175.5
(3)
P 		98.7
(-2.3)
P 		88.5
(-3)
P 		91.6
(-2.8)
P 		87
C 176
(3)
P 		104.1
(-1.1)
P 		138.5
(0.8)
P 		71.5
(-3)
P 		105
avr 182.7
(3) 		92.8
(-1.8) 		103.8
(-1.2) 		69.6
(-3) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 83.1
(-3)
P 		172.8
(1.4)
P 		105
(-1.9)
P 		136.1
(-0.4)
P 		168.7
(1.2)
P 		204.8
(3)
P 		122
B 162
(-1.5)
P 		150.3
(-1.9)
P 		123.3
(-3)
P 		129.7
(-2.7)
P 		241.5
(1.6)
P 		276
(3)
P 		153
C 152.6
(-1.6)
P 		137.1
(-2.1)
P 		117.2
(-2.8)
P 		111.8
(-3)
P 		156.5
(-1.5)
P 		285.8
(3)
P 		174
avr 132.6
(-2.3) 		153.4
(-1.4) 		115.2
(-3) 		125.9
(-2.5) 		188.9
(0.2) 		255.5
(3) 		140
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190892_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3759
Sequence Type Exemplar sequence
Representative Public ID E02H1.4 NCBI
Target Description E02H1.4 /REP_DB=WormBase Gene ID /WP=CE01539 /TR=SW:Q09525 /GB=CAA87379.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=ADP-Ribosyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026886(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026886 NCBI Caenorhabditis elegans E02H1.4 (ADP-Ribosyltransferase) mRNA, complete cds. 11/11 None
SNAP00000041922 ENSEMBL cdna:SNAP chromosome:CEL140:II:9592957:9594804:1 11/11 None
GENEFINDER00000041933 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9592957:9594804:1 11/11 None
E02H1.4 ENSEMBL cdna:known chromosome:CEL140:II:9592947:9595040:1 gene:E02H1.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9592953-9594801(+) 100.0 100.0
Public Domain and Genome References
Gene Title ADP-Ribosyltransferase
Gene Symbol pme-2
Ensembl E02H1.4
Entrez Gene 3565967 Entrez gene
SwissProt Q09525 EMBL-EBI
WormBase CE01539 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6471 protein amino acid ADP-ribosylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3950 NAD+ ADP-ribosyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87379 Hypothetical protein E02H1.4 [Caenorhabditis elegans] ref|NP_001022057.1| E02H1.4 [Caenorhabditis elegans] gb|AAM27196.1| poly ADP-ribose metabolism enzyme-2 [Caenorhabditis elegans] sp|Q09525|PME2_CAEEL Poly(ADP-ribose) polymerase pme-2 (Poly ADP-ribose metabolism enzyme 2) 0.0
blast CAE57909 Hypothetical protein CBG00959 [Caenorhabditis briggsae] 0.0
blast XP_520665 PREDICTED: similar to poly-(ADP-ribose) polymerase II [Pan troglodytes] 5.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR001290 EMBL-EBI Poly(ADP-ribose) polymerase, catalytic region 1.0E-126
Pfam IPR008893 EMBL-EBI WGR 4.6E-36
Pfam IPR004102 EMBL-EBI Poly(ADP-ribose) polymerase, regulatory domain 5.6E-77
Sequence
Target Sequence 
>C. ELEGANS:190892_AT
agagcacaaagaccgctgctccaactgttccaccaccaactacaaaacgccttctttggc
acggtactcgtgtgacaaatgtcttctcaatcctcatgaatggacttcagttcccagttg
gagatcgctgtggtctgatgttcggaaatggagtttatttcgcaaatgttccaactaaat
ccgcgaattactgttgcccagaggcttcaaagagagttttcatgcttctttgcgaggttg
aaactgcgaacccattagttctctacgagtcagaaattgacgcggatgagaaaatggaaa
aagcgaaaaagacgtcggtttatgcagctggaaagcacactccaagagacactgttgaaa
tcaatggaatcccggcattcaagtcaaatcttgagacaatcgaagaagaaactcgtcttc
tctacgatgaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGCACAAAGACCGCTGCTCCAAC 403 69 1109 Antisense
TACAAAACGCCTTCTTTGGCACGGT 194 643 1149 Antisense
ATGTCTTCTCAATCCTCATGAATGG 384 47 1187 Antisense
AATGGACTTCAGTTCCCAGTTGGAG 17 163 1207 Antisense
TGGAGATCGCTGTGGTCTGATGTTC 146 549 1227 Antisense
AATCCGCGAATTACTGTTGCCCAGA 19 169 1286 Antisense
GAGTTTTCATGCTTCTTTGCGAGGT 57 399 1322 Antisense
ACTGCGAACCCATTAGTTCTCTACG 525 97 1351 Antisense
AAGACGTCGGTTTATGCAGCTGGAA 447 151 1417 Antisense
AATCCCGGCATTCAAGTCAAATCTT 24 169 1476 Antisense
GAAACTCGTCTTCTCTACGATGAAT 500 355 1516 Antisense
Sequence Source Affymetrix Proprietary Database