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[190891_at] Beta-mannosyltransferase

Description T26A5.4 /REP_DB=WormBase Gene ID /WP=CE00701 /TR=Q22797 /GB=AAC77507.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Beta-mannosyltransferase
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 85      4grp_highest_8_8 > 35      4grp_high_8_8 > 56      6grp_highest_nonorm > 95      6grp_total > 75      4grp_total > 63      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 560.2
(3)
P 		496.9
(-3)
P 		516.2
(-1.2)
P 		509.5
(-1.8)
P 		63
B 584.7
(3)
P 		476.5
(-2.5)
P 		517.5
(-0.4)
P 		466.4
(-3)
P 		118
C 559.8
(3)
P 		448.6
(-3)
P 		530.1
(1.4)
P 		481
(-1.3)
P 		111
avr 568.2
(3) 		474
(-3) 		521.3
(0) 		485.6
(-2.3) 		94

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 686
1 2 3 4 5 6 range
A 433.3
(-3)
P 		474.9
(-2.5)
P 		596.7
(-0.9)
P 		701.1
(0.4)
P 		660.5
(-0.1)
P 		904.5
(3)
P 		471
B 385
(-3)
P 		509.3
(-1.7)
P 		825.2
(1.7)
P 		720.8
(0.6)
P 		692.4
(0.3)
P 		942.5
(3)
P 		558
C 399.5
(-3)
P 		502.4
(-2.1)
P 		567.2
(-1.5)
P 		779.6
(0.4)
P 		672.3
(-0.6)
P 		1070.5
(3)
P 		671
avr 405.9
(-3) 		495.5
(-2.1) 		663
(-0.3) 		733.8
(0.5) 		675.1
(-0.1) 		972.5
(3) 		567
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 204
(3)
P 		107.9
(-1.5)
P 		134.9
(-0.2)
P 		74.6
(-3)
P 		129
B 186.5
(3)
P 		117.7
(-1.7)
P 		119.9
(-1.6)
P 		99.4
(-3)
P 		87
C 185.3
(3)
P 		129
(-0.2)
P 		184.8
(3)
P 		81.1
(-3)
P 		104
avr 191.9
(3) 		118.2
(-1.1) 		146.5
(0.5) 		85
(-3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 279
1 2 3 4 5 6 range
A 79.9
(-3)
P 		168.9
(0.8)
P 		130
(-0.9)
P 		177.8
(1.2)
P 		199.3
(2.1)
P 		220.2
(3)
P 		140
B 133.9
(-3)
P 		169.5
(-2)
P 		200.4
(-1.2)
P 		190.9
(-1.4)
P 		281.3
(1.1)
P 		351.4
(3)
P 		218
C 148.1
(-2.9)
P 		153.3
(-2.8)
P 		144.4
(-3)
P 		156.8
(-2.7)
P 		217.5
(-1)
P 		358.4
(3)
P 		214
avr 120.6
(-3) 		163.9
(-1.6) 		158.3
(-1.8) 		175.2
(-1.3) 		232.7
(0.6) 		310
(3) 		189
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190891_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6293
Sequence Type Exemplar sequence
Representative Public ID T26A5.4 NCBI
Target Description T26A5.4 /REP_DB=WormBase Gene ID /WP=CE00701 /TR=Q22797 /GB=AAC77507.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Beta-mannosyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066019(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066019 NCBI Caenorhabditis elegans T26A5.4 (Beta-mannosyltransferase) mRNA, complete cds. 11/11 None
SNAP00000033267 ENSEMBL cdna:SNAP chromosome:CEL140:III:6460064:6462119:1 11/11 None
GENEFINDER00000033287 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6460064:6462119:1 11/11 None
T26A5.4 ENSEMBL cdna:known chromosome:CEL140:III:6460057:6462142:1 gene:T26A5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6460062-6462118(+) 100.0 100.0
Public Domain and Genome References
Gene Title Beta-mannosyltransferase
Ensembl T26A5.4
Entrez Gene 175920 Entrez gene
SwissProt Q22797 EMBL-EBI
WormBase CE00701 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246351_AT glycosyl transferase family 1 protein at
CANINE_2:CFAAFFX.29186.1.S1_AT similar to beta-1,4-mannosyltransferase cfa
CANINE_2:CFAAFFX.29186.1.S1_S_AT similar to beta-1,4-mannosyltransferase cfa
DROSOPHILA_2:1638157_AT dm
DROSGENOME1:154107_AT dm
CHICKEN:GGAAFFX.1366.1.S1_S_AT similar to Beta-1,4-mannosyltransferase gga
CHICKEN:GGAAFFX.1366.3.S1_AT similar to Beta-1,4-mannosyltransferase gga
HG-U133B:223355_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) hs
HG-U133_PLUS_2:223355_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) hs
HU35KSUBA:AA464368_S_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) hs
HU35KSUBA:AA464386_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) hs
HU35KSUBC:RC_AA464702_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) hs
U133_X3P:G13325167_3P_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) hs
HG-U95C:61309_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) hs
HG-U95C:64026_F_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) hs
MOE430A:1451368_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) mm
MOUSE430A_2:1451368_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) mm
MOUSE430_2:1451368_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) mm
MU19KSUBA:TC19149_AT Asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (Alg1), mRNA mm
RG-U34B:RC_AA946316_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (predicted) rn
RAE230A:1373107_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (predicted) rn
RAT230_2:1373107_AT asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (predicted) rn
RAE230B:1396370_AT Asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (predicted) rn
RAT230_2:1396370_AT Asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (predicted) rn
YEAST_2:1769684_AT Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9058 biosynthesis inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_498420 T26A5.4 [Caenorhabditis elegans] gb|AAC77507.1| Hypothetical protein T26A5.4 [Caenorhabditis elegans] 0.0
blast CAE72501 Hypothetical protein CBG19680 [Caenorhabditis briggsae] 0.0
blast NP_498420 T26A5.4 [Caenorhabditis elegans] gb|AAC77507.1| Hypothetical protein T26A5.4 [Caenorhabditis elegans] 1.0E-179
blast CAE72501 Hypothetical protein CBG19680 [Caenorhabditis briggsae] 1.0E-125
blast AAF55475 CG18012-PA [Drosophila melanogaster] gb|AAM50778.1| LD22559p [Drosophila melanogaster] ref|NP_650662.1| CG18012-PA [Drosophila melanogaster] 3.0E-86
blast AAF55475 CG18012-PA [Drosophila melanogaster] gb|AAM50778.1| LD22559p [Drosophila melanogaster] ref|NP_650662.1| CG18012-PA [Drosophila melanogaster] 3.0E-85
Protein Domains
Database ID Description E-Value
Pfam IPR001296 EMBL-EBI Glycosyl transferase, group 1 2.5E-14
Pfam IPR001296 EMBL-EBI Glycosyl transferase, group 1 2.5E-14
Pfam IPR001296 EMBL-EBI Glycosyl transferase, group 1 2.5E-14
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498420.1 3 79-101,108-130,462-484
T26A5.4 3 79-101,108-130,462-484
SNAP00000033267 1 360-382
GENEFINDER00000033287 3 79-101,108-130,466-488
Sequence
Target Sequence 
>C. ELEGANS:190891_AT
tgaaattcttctcgatgcgctggttgcatatgacaaaacaatcggactcccgcgagtttt
gatgattatcactggaaaaggtccactgaaagcgaaatatttacaagaaattcacgagaa
aaatctgaagaatgtcgatgtattgactccatggttggaagcagaagattatccaaagat
tctagcctctgctgacttaggaatctctttgcacacaagcacgtctggattggatcttcc
gatgaaagttgttgatatgtttggagctaaagttccagcattggctctaaaattcaaatg
tattgatgagctcgttgaggagaaaactaatggatatcttttcgatgattctgagcagtt
gagtcgtcaaattattgaactttctcgtggattcccaaataattgcaatgaattgatacg
gctcaagaagaatacacaggagcaaaaattcgattcttgggaagtgatgtggaagagatc
agctacctctggtgcagactttcgagttccagacaatggattcgccaaacttcgtgcaat
tattcaattctcatttttcgctattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAATTCTTCTCGATGCGCTGGTT 700 571 849 Antisense
AATCGGACTCCCGCGAGTTTTGATG 205 169 888 Antisense
TTATCCAAAGATTCTAGCCTCTGCT 409 681 1017 Antisense
AGCCTCTGCTGACTTAGGAATCTCT 521 83 1032 Antisense
GAATCTCTTTGCACACAAGCACGTC 235 329 1049 Antisense
GCACGTCTGGATTGGATCTTCCGAT 482 315 1067 Antisense
ATTGAACTTTCTCGTGGATTCCCAA 498 5 1222 Antisense
GAAGAGATCAGCTACCTCTGGTGCA 542 335 1320 Antisense
GTGCAGACTTTCGAGTTCCAGACAA 144 481 1340 Antisense
GATTCGCCAAACTTCGTGCAATTAT 480 433 1367 Antisense
ATTCAATTCTCATTTTTCGCTATTT 168 13 1390 Antisense
Sequence Source Affymetrix Proprietary Database