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[190867_s_at] Mouse MSMAD protein like

Description F01G10.8 /REP_DB=WormBase Gene ID /WP=CE27913 /TR=O17760 /GB=CAB02890.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Mouse MSMAD protein like
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 36      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 74      6grp_total > 36      4grp_total > 14      6grp_highest_sub > Cu      6grp_cgc > 15      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1171
1 2 3 4 range
A 998
(0.5)
P 		462.2
(-3)
P 		560.9
(-2.4)
P 		1389.5
(3)
P 		927
B 981.4
(-0.3)
P 		536.1
(-2.6)
P 		460.2
(-3)
P 		1631
(3)
P 		1171
C 1246.5
(3)
P 		541.1
(-2.6)
P 		496
(-3)
P 		1141.5
(2.2)
P 		751
avr 1075.3
(0.9) 		513.1
(-2.9) 		505.7
(-3) 		1387.3
(3) 		882

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 366
1 2 3 4 5 6 range
A 816.9
(3)
P 		774.6
(2.1)
P 		753.7
(1.6)
P 		546.4
(-3)
P 		570.7
(-2.5)
P 		668.9
(-0.3)
P 		271
B 814.4
(2.6)
P 		835.7
(3)
P 		506.2
(-3)
P 		564.8
(-1.9)
P 		523.6
(-2.7)
P 		769.9
(1.8)
P 		330
C 758.6
(3)
P 		757.3
(3)
P 		660.2
(1)
P 		598.5
(-0.3)
P 		469.4
(-3)
P 		716.9
(2.1)
P 		289
avr 796.6
(3) 		789.2
(2.8) 		640
(-0.4) 		569.9
(-1.9) 		521.2
(-3) 		718.6
(1.3) 		275
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 312
1 2 3 4 range
A 363.4
(3)
P 		100.3
(-3)
P 		146.6
(-1.9)
P 		203.4
(-0.6)
P 		263
B 313
(2.1)
P 		132.4
(-2.4)
P 		106.6
(-3)
P 		347.6
(3)
P 		241
C 412.6
(3)
P 		155.6
(-3)
P 		172.9
(-2.6)
P 		192.5
(-2.1)
P 		257
avr 363
(3) 		129.4
(-3) 		142
(-2.7) 		247.8
(0) 		234

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 167
1 2 3 4 5 6 range
A 150.6
(-2.5)
P 		275.5
(3)
P 		164.1
(-1.9)
P 		138.6
(-3)
P 		172.2
(-1.5)
P 		162.9
(-1.9)
P 		137
B 283.4
(2.9)
P 		278.2
(2.7)
P 		122.9
(-3)
P 		149.6
(-2)
P 		212.7
(0.3)
P 		287.1
(3)
P 		164
C 281.2
(3)
P 		231.1
(1.1)
P 		168
(-1.2)
P 		120.4
(-3)
P 		151.9
(-1.8)
P 		240
(1.5)
P 		161
avr 238.4
(1.9) 		261.6
(3) 		151.7
(-2.3) 		136.2
(-3) 		178.9
(-1) 		230
(1.5) 		125
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190867_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9322
Sequence Type Exemplar sequence
Representative Public ID F01G10.8 NCBI
Target Description F01G10.8 /REP_DB=WormBase Gene ID /WP=CE27913 /TR=O17760 /GB=CAB02890.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Mouse MSMAD protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069479(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069479 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-14) (daf-14) mRNA, complete cds. 11/11 A 
SNAP00000013163 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10254098:10255566:1 11/11 None
GENEFINDER00000013166 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10250617:10255566:1 11/11 None
F01G10.8 ENSEMBL cdna:known chromosome:CEL140:IV:10253588:10255808:1 gene:F01G10.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10254103-10254996(+) 100.0 100.0
Public Domain and Genome References
Gene Title Mouse MSMAD protein like
Gene Symbol daf-14
Ensembl F01G10.8
Entrez Gene 177908 Entrez gene
SwissProt O17760 EMBL-EBI
WormBase CE29090 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02890 Hypothetical protein F01G10.8 [Caenorhabditis elegans] ref|NP_501880.1| abnormal DAuer Formation family member (daf-14) [Caenorhabditis elegans] gb|AAF03892.1| Smad protein [Caenorhabditis elegans] 1.0E-152
blast CAB02890 Hypothetical protein F01G10.8 [Caenorhabditis elegans] ref|NP_501880.1| abnormal DAuer Formation family member (daf-14) [Caenorhabditis elegans] gb|AAF03892.1| Smad protein [Caenorhabditis elegans] 1.0E-141
blast NP_778258 MAD, mothers against decapentaplegic homolog 3b [Danio rerio] gb|AAN08604.1| Smad3b [Danio rerio] 1.0E-21
blast AAH81628 MAD, mothers against decapentaplegic homolog 3b [Danio rerio] 1.0E-21
blast XP_594136 PREDICTED: similar to MAD homolog 2 isoform 1 [Bos taurus] 2.0E-21
blast XP_424724 PREDICTED: similar to MADH2 protein [Gallus gallus] 8.0E-21
Protein Domains
Database ID Description E-Value
Pfam IPR001132 EMBL-EBI Dwarfin protein 8.3E-18
Pfam IPR001132 EMBL-EBI Dwarfin protein 2.9E-13
Pfam IPR001132 EMBL-EBI Dwarfin protein 8.3E-18
Pfam IPR001132 EMBL-EBI Dwarfin protein 2.9E-13
Pfam IPR001132 EMBL-EBI Dwarfin protein 8.3E-18
Pfam IPR001132 EMBL-EBI Dwarfin protein 2.9E-13
Sequence
Target Sequence 
>C. ELEGANS:190867_S_AT
gaagtacgtcctactggaaacgagatgttggatcctgaacccaaatatccaaaagaagaa
aaaccgtggtgtaccatattttactacgagctgactgttcgactaggaaaagcattcgaa
gccaaagtcccaacaattacaatcgatggtgcaactggagcaagtgatgagtgccgaatg
tcgcttacatctcaaccatcttcgagaaattcaaaaagctcacaaatccgaaacacagtt
ggagcaggaattcaattggcatatgaaaatggagaactttggctaactgtactgactgac
caaattgtttttgtacaatgcccattcctgaaccagacattgaataagccactgaaatat
gtgtttcggttgcaaaacaaaggagatcaaaaaagaatgaaaatattcgataaggagcag
tttgaacaggagaaaacattggctctcggacctttaactgaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGTACGTCCTACTGGAAACGAGA 164 335 166 Antisense
AACGAGATGTTGGATCCTGAACCCA 212 143 184 Antisense
ACTACGAGCTGACTGTTCGACTAGG 551 101 248 Antisense
TCGAAGCCAAAGTCCCAACAATTAC 407 601 281 Antisense
AAGTGATGAGTGCCGAATGTCGCTT 421 157 327 Antisense
GAATGTCGCTTACATCTCAACCATC 401 331 341 Antisense
AAAGCTCACAAATCCGAAACACAGT 106 125 380 Antisense
GGCTAACTGTACTGACTGACCAAAT 699 537 446 Antisense
GTTTTTGTACAATGCCCATTCCTGA 152 449 472 Antisense
TGCCCATTCCTGAACCAGACATTGA 319 583 484 Antisense
TTGGCTCTCGGACCTTTAACTGAAA 563 707 604 Antisense
Sequence Source Affymetrix Proprietary Database