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[190859_s_at]

Description F35G12.3A /REP_DB=WormBase Gene ID /WP=CE00971 /TR=Q20050 /GB=CAA86326.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_highest_10_10 > 85      6grp_high1_10_10 > 68      4grp_high_8_8 > 01      6grp_highest_nonorm > 91      6grp_total > 47      4grp_total > 23      6grp_highest_sub > Cu      6grp_cgc > 19      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 151.1
(3)
P 		90.7
(-3)
A 		90.8
(-3)
A 		131
(1)
P 		60
B 178
(3)
P 		119.1
(-1.7)
P 		102.9
(-3)
P 		152.9
(1)
P 		75
C 155.9
(0.9)
P 		119.5
(-3)
P 		151.5
(0.5)
P 		175.1
(3)
P 		56
avr 161.7
(3) 		109.8
(-3) 		115.1
(-2.4) 		153
(2) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 228.3
(3)
P 		203.8
(1.2)
P 		151.8
(-2.6)
P 		146.2
(-3)
P 		200.2
(0.9)
P 		221.2
(2.5)
P 		82
B 163.3
(-2.2)
P 		271.7
(3)
P 		145.8
(-3)
P 		244.9
(1.7)
P 		161.6
(-2.2)
P 		261.5
(2.5)
P 		126
C 193.1
(-0.7)
P 		207.7
(-0.2)
P 		130.8
(-3)
P 		200.3
(-0.4)
P 		157.2
(-2)
P 		293.8
(3)
P 		163
avr 194.9
(-0.3) 		227.7
(1.4) 		142.8
(-3) 		197.1
(-0.2) 		173
(-1.4) 		258.8
(3) 		116
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 55
(3)
P 		19.7
(-2.9)
A 		23.7
(-2.2)
A 		19.2
(-3)
P 		36
B 56.8
(3)
P 		29.4
(-2)
P 		23.8
(-3)
P 		32.6
(-1.4)
P 		33
C 51.6
(2.7)
P 		34.4
(-1.7)
P 		52.8
(3)
P 		29.5
(-3)
P 		23
avr 54.5
(3) 		27.8
(-2.8) 		33.4
(-1.6) 		27.1
(-3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 42.1
(-1.6)
P 		72.5
(3)
P 		33.1
(-3)
P 		37.1
(-2.4)
P 		60.4
(1.2)
P 		53.8
(0.2)
P 		39
B 56.8
(-0.9)
P 		90.4
(2.3)
P 		35.4
(-3)
P 		64.9
(-0.1)
P 		65.7
(-0.1)
P 		97.5
(3)
P 		62
C 71.6
(0.5)
P 		63.4
(-0.2)
P 		33.3
(-3)
P 		40.3
(-2.4)
P 		50.9
(-1.4)
P 		98.4
(3)
P 		65
avr 56.8
(-0.2) 		75.4
(2.1) 		33.9
(-3) 		47.4
(-1.4) 		59
(0.1) 		83.2
(3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190859_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7048
Sequence Type Exemplar sequence
Representative Public ID F35G12.3A NCBI
Target Description F35G12.3A /REP_DB=WormBase Gene ID /WP=CE00971 /TR=Q20050 /GB=CAA86326.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027391(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027391 NCBI Caenorhabditis elegans Suppressor/Enhancer of Lin-12 family member (sel-5) (sel-5) mRNA, complete cds. 11/11 None
SNAP00000042237 ENSEMBL cdna:SNAP chromosome:CEL140:III:4572081:4578392:-1 11/11 None
GENEFINDER00000042263 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4572081:4578392:-1 11/11 None
F35G12.3a ENSEMBL cdna:known chromosome:CEL140:III:4571780:4578392:-1 gene:F35G12.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
F35G12.3b 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175599 2 1 NM_001027391 173477_at (11), 190859_s_at (11)
NM_001027392 173478_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4572080-4578392(-) 97.12 100.0
Public Domain and Genome References
Ensembl F35G12.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86326 Hypothetical protein F35G12.3a [Caenorhabditis elegans] ref|NP_001022562.1| Suppressor/Enhancer of Lin-12 family member (sel-5) [Caenorhabditis elegans] gb|AAF71545.1| SEL-5A serine/threonine kinase [Caenorhabditis elegans] 0.0
blast CAE71182 Hypothetical protein CBG18039 [Caenorhabditis briggsae] 0.0
blast CAA86332 Hypothetical protein F35G12.3b [Caenorhabditis elegans] ref|NP_001022563.1| Suppressor/Enhancer of Lin-12 family member (sel-5) [Caenorhabditis elegans] gb|AAF71546.1| SEL-5B serine/threonine kinase [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.9E-36
Sequence
Target Sequence 
>C. ELEGANS:190859_S_AT
agcaactccgttggtttgtcgatttaagccggatctacccacagctgctcctgtttcaat
aattccatcaatgtcaaatacatcgtttcctgaagctgttcgagactctgatacagtccc
ttgtgagccaattattggaactttaatttctgttggcgcacctacggatccaccaccacc
acctcttccaaagaaaccaacagaagcttctccaacacaagagacgactgcaacgattcc
agtggctttgggtaaaaaggagaaattgttgaaaaaggagaagaaaaaagagaaaaaaga
tgggaagaaggataaattaaaattagaagaatatcgagaaaaaggatcctctgaacctga
aaccgatggatctgaagctgaaatatggacgaatgatggagcaacaactttttcgaataa
gaaaaagaaaaagagcacatttggattacgatcatcacatccatcaattgttgcaaatga
tttgcagttctcttctccaatgcctccagttgttaaaaaatcatccaaagataaaaaatc
gtcactgactggcaaaaatgcatcatttgtgaatacatccttccaaccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCAACTCCGTTGGTTTGTCGATTT 141 73 2628 Antisense
TGTCGATTTAAGCCGGATCTACCCA 561 557 2644 Antisense
CAGCTGCTCCTGTTTCAATAATTCC 569 203 2669 Antisense
GTTTCCTGAAGCTGTTCGAGACTCT 528 449 2712 Antisense
TCTGATACAGTCCCTTGTGAGCCAA 587 607 2734 Antisense
TTTAATTTCTGTTGGCGCACCTACG 525 673 2769 Antisense
GACGACTGCAACGATTCCAGTGGCT 195 379 2850 Antisense
GATCCTCTGAACCTGAAACCGATGG 391 415 2972 Antisense
ATCATCACATCCATCAATTGTTGCA 186 31 3078 Antisense
AAATGATTTGCAGTTCTCTTCTCCA 134 121 3102 Antisense
ATTTGTGAATACATCCTTCCAACCG 293 17 3192 Antisense
Sequence Source Affymetrix Proprietary Database