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[190838_at] Phosphatase/kinase domain

Description F25H2.2 /REP_DB=WormBase Gene ID /WP=CE09647 /TR=Q93566 /GB=CAB02091.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Phosphatase-kinase domain
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 22      4grp_highest_8_8 > 53      4grp_high_8_8 > 17      6grp_highest_nonorm > 61      6grp_total > 22      4grp_total > 31      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 431
1 2 3 4 range
A 628.9
(3)
P 		300.7
(-2)
P 		236.7
(-3)
P 		530.6
(1.5)
P 		392
B 622.9
(3)
P 		301.7
(-2.6)
P 		281.4
(-3)
P 		416.1
(-0.6)
P 		342
C 667.4
(3)
P 		332.3
(-3)
P 		332.4
(-3)
P 		564.9
(1.2)
P 		335
avr 639.7
(3) 		311.6
(-2.5) 		283.5
(-3) 		503.9
(0.7) 		356

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 366
1 2 3 4 5 6 range
A 530.5
(3)
P 		496.5
(2.3)
P 		500.2
(2.3)
P 		408.6
(0.3)
P 		406.6
(0.3)
P 		257.1
(-3)
P 		273
B 579.5
(3)
P 		570.1
(2.8)
P 		562.4
(2.6)
P 		413
(-0.7)
P 		467.7
(0.5)
P 		309.7
(-3)
P 		270
C 478.4
(0.4)
P 		623.3
(3)
P 		403.5
(-0.9)
P 		475.7
(0.3)
P 		345.7
(-2)
P 		289.4
(-3)
P 		334
avr 529.5
(2.3) 		563.3
(3) 		488.7
(1.4) 		432.4
(0.2) 		406.7
(-0.4) 		285.4
(-3) 		278
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 229
(3)
P 		65.3
(-2.9)
P 		61.9
(-3)
P 		77.7
(-2.4)
P 		167
B 198.6
(3)
P 		74.5
(-2.6)
P 		65.2
(-3)
P 		88.7
(-1.9)
P 		133
C 220.9
(3)
P 		95.5
(-3)
P 		115.9
(-2)
P 		95.3
(-3)
P 		126
avr 216.2
(3) 		78.4
(-3) 		81
(-2.9) 		87.2
(-2.6) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 139
1 2 3 4 5 6 range
A 97.8
(-1.1)
P 		176.6
(3)
P 		108.9
(-0.6)
P 		103.6
(-0.8)
P 		122.7
(0.2)
P 		62.6
(-3)
P 		114
B 201.6
(3)
P 		189.8
(2.2)
P 		136.6
(-1.2)
P 		109.4
(-3)
P 		190
(2.2)
P 		115.5
(-2.6)
P 		92
C 177.3
(2.2)
P 		190.2
(3)
P 		102.7
(-2.6)
P 		95.7
(-3)
P 		111.9
(-2)
P 		96.9
(-2.9)
P 		95
avr 158.9
(1.3) 		185.5
(3) 		116.1
(-1.4) 		102.9
(-2.3) 		141.5
(0.2) 		91.7
(-3) 		94
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190838_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2171
Sequence Type Exemplar sequence
Representative Public ID F25H2.2 NCBI
Target Description F25H2.2 /REP_DB=WormBase Gene ID /WP=CE09647 /TR=Q93566 /GB=CAB02091.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Phosphatase-kinase domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060357(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060357 NCBI Caenorhabditis elegans F25H2.2 (F25H2.2) mRNA, complete cds. 11/11 None
SNAP00000042615 ENSEMBL cdna:SNAP chromosome:CEL140:I:10551147:10554306:1 11/11 None
GENEFINDER00000042635 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10551147:10554306:1 11/11 None
F25H2.2 ENSEMBL cdna:known chromosome:CEL140:I:10551145:10554546:1 gene:F25H2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10551077-10554237(+) 100.0 100.0
Public Domain and Genome References
Gene Title Phosphatase/kinase domain
Ensembl F25H2.2
Entrez Gene 172937 Entrez gene
SwissProt Q93566 EMBL-EBI
WormBase CE09647 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604708_AT similar to PDZ protein Mrt1 cfa
CANINE_2:CFA.1942.1.S1_AT similar to PDZ protein Mrt1 cfa
CANINE_2:CFAAFFX.19759.1.S1_AT similar to PDZ protein Mrt1 cfa
DROSOPHILA_2:1637506_AT dm
DROSOPHILA_2:1627179_S_AT dm
DROSGENOME1:151385_AT dm
DROSGENOME1:144544_AT dm
DROSGENOME1:142995_AT dm
CHICKEN:GGA.7093.1.S1_S_AT sorting nexin family member 27 gga
CHICKEN:GGAAFFX.12726.1.S1_AT sorting nexin family member 27 gga
CHICKEN:GGA.7093.1.S1_AT Similar to mKIAA0488 protein gga
HG-U133_PLUS_2:221006_S_AT sorting nexin family member 27 hs
HG-U133A:221006_S_AT sorting nexin family member 27 hs
HG-U133A_2:221006_S_AT sorting nexin family member 27 hs
HU35KSUBA:AA089559_AT sorting nexin family member 27 hs
HU35KSUBA:RC_AA398421_S_AT sorting nexin family member 27 hs
HU35KSUBC:RC_T90750_AT sorting nexin family member 27 hs
HU35KSUBD:RC_AA427953_AT sorting nexin family member 27 hs
U133_X3P:G13569876_3P_S_AT sorting nexin family member 27 hs
U133_X3P:HS.67619.0.S2_3P_AT sorting nexin family member 27 hs
HG-U133A_2:221498_AT sorting nexin family member 27 hs
HG-U133_PLUS_2:221498_AT sorting nexin family member 27 hs
HG-U133A:221498_AT sorting nexin family member 27 hs
HG-U95AV2:32504_AT sorting nexin family member 27 hs
HG-U95AV2:32503_AT sorting nexin family member 27 hs
HG-U133_PLUS_2:244349_AT Sorting nexin family member 27 hs
HG-U133B:244349_AT Sorting nexin family member 27 hs
HG-U95D:84186_AT Sorting nexin family member 27 hs
HG-U95E:85894_AT Sorting nexin family member 27 hs
HU35KSUBB:RC_N30674_AT Sorting nexin family member 27 hs
U133_X3P:HS.192326.0.A1_3P_AT Sorting nexin family member 27 hs
MU11KSUBA:C79375_RC_AT sorting nexin family member 27 mm
MG-U74BV2:108075_AT sorting nexin family member 27 mm
MG-U74BV2:108872_AT sorting nexin family member 27 mm
MG-U74CV2:166647_AT sorting nexin family member 27 mm
MOE430B:1433614_AT sorting nexin family member 27 mm
MOUSE430_2:1433614_AT sorting nexin family member 27 mm
MOE430B:1430037_AT sorting nexin family member 27 mm
MOUSE430_2:1430037_AT sorting nexin family member 27 mm
MG-U74AV2:95980_AT sorting nexin family member 27 mm
MU19KSUBA:TC17789_AT Sorting nexin family member 27, mRNA (cDNA clone IMAGE:6431126) mm
MU19KSUBB:TC31497_AT Sorting nexin family member 27, mRNA (cDNA clone IMAGE:6431126) mm
RG-U34B:RC_AA900057_AT PDZ protein Mrt1 rn
RG-U34C:RC_AI101286_AT PDZ protein Mrt1 rn
RAE230A:1377526_AT PDZ protein Mrt1 rn
RAT230_2:1377526_AT PDZ protein Mrt1 rn
RAE230A:1372203_AT PDZ protein Mrt1 rn
RAT230_2:1372203_AT PDZ protein Mrt1 rn
RAE230B:1381394_AT PDZ protein Mrt1 rn
RAT230_2:1381394_AT PDZ protein Mrt1 rn
RAE230B:1379804_AT PDZ protein Mrt1 rn
RAT230_2:1379804_AT PDZ protein Mrt1 rn
RAE230B:1395216_AT PDZ protein Mrt1 rn
RAT230_2:1395216_AT PDZ protein Mrt1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
5515 protein binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02091 Hypothetical protein F25H2.2 [Caenorhabditis elegans] ref|NP_492758.1| F25H2.2 [Caenorhabditis elegans] 0.0
blast CAE58970 Hypothetical protein CBG02242 [Caenorhabditis briggsae] 0.0
blast EAL38656 ENSANGP00000027258 [Anopheles gambiae str. PEST] gb|EAA00958.2| ENSANGP00000011959 [Anopheles gambiae str. PEST] ref|XP_322054.2| ENSANGP00000011959 [Anopheles gambiae str. PEST] ref|XP_551739.1| ENSANGP00000027258 [Anopheles gambiae str. PEST] 1.0E-118
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.6E-14
Pfam IPR001683 EMBL-EBI Phox-like 5.4E-32
Pfam IPR001683 EMBL-EBI Phox-like 1.9E-27
Sequence
Target Sequence 
>C. ELEGANS:190838_AT
gagacatggtcttcaaacaattctgttttgtgcaagcaattgcggatttacacagcggaa
aagtggtgacatcaaggaaaagctatcagttgagagcaatacaaaatgaggagaatatgg
atgagttgttggagatggcacgggaacttgaaggttacaatcaggtcacttttccgccaa
gtgtgtgtattttgaggaaacgaagacaaactgtcattatggttgtgaggtttgcaaatc
tgttgctcaagtgtccagaagatggaaactcgatggaaatcgattggagtcgaatcaaag
actttcgagtttgtgatgaagccaccgcattctcatttgagtacattagagatgaagttg
gagatagtgagcaagagcaaacggagccacggaaaacaaaatatgttcgattggaaactg
gatttgctgagtacatggcagaatgctttgcggaaattcaattcgaacggcaagctggat
ctgattggaaaagagatcaaaatcgtgttgctgcaactccagaaccacttccagaaacta
tagaaccacaaccaactactcataaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGACATGGTCTTCAAACAATTCTG 542 393 1133 Antisense
GCAATTGCGGATTTACACAGCGGAA 419 323 1168 Antisense
GTTACAATCAGGTCACTTTTCCGCC 548 443 1286 Antisense
CTTTTCCGCCAAGTGTGTGTATTTT 471 221 1301 Antisense
GCAAATCTGTTGCTCAAGTGTCCAG 474 317 1366 Antisense
TCGAGTTTGTGATGAAGCCACCGCA 386 601 1437 Antisense
AGCCACCGCATTCTCATTTGAGTAC 173 85 1452 Antisense
GAGCAAGAGCAAACGGAGCCACGGA 351 391 1501 Antisense
ATTCGAACGGCAAGCTGGATCTGAT 552 5 1593 Antisense
GATCAAAATCGTGTTGCTGCAACTC 546 421 1627 Antisense
AGAACCACAACCAACTACTCATAAA 607 73 1674 Antisense
Sequence Source Affymetrix Proprietary Database