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[190833_at]

Description F35G12.11 /REP_DB=WormBase Gene ID /WP=CE00969 /TR=Q20057 /GB=CAA86333.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Enhancer of rudimentary
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 97      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 96      6grp_total > 97      4grp_total > 50      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 284
1 2 3 4 range
A 527.1
(-3)
P 		598.1
(-1)
P 		737.3
(3)
P 		536.2
(-2.7)
P 		210
B 473.9
(-2.5)
P 		716.4
(3)
P 		610.8
(0.6)
P 		453
(-3)
P 		263
C 529.1
(-1.6)
P 		682.4
(3)
P 		482.6
(-3)
P 		557.1
(-0.8)
P 		200
avr 510
(-3) 		665.6
(3) 		610.2
(0.9) 		515.4
(-2.8) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 905
1 2 3 4 5 6 range
A 586.8
(-3)
P 		602
(-2.8)
P 		581.3
(-3)
P 		669.4
(-2.3)
P 		984.2
(0.2)
P 		1329.9
(3)
P 		749
B 623
(-2.6)
P 		607.3
(-2.7)
P 		561.2
(-3)
P 		665.9
(-2.3)
P 		1143.3
(1)
P 		1426.6
(3)
P 		865
C 568.4
(-2.8)
P 		705.8
(-1.9)
P 		536.2
(-3)
P 		1102.9
(0.8)
P 		898.1
(-0.6)
P 		1441.4
(3)
P 		905
avr 592.7
(-2.8) 		638.4
(-2.4) 		559.6
(-3) 		812.7
(-1.2) 		1008.5
(0.2) 		1399.3
(3) 		840
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 191.9
(3)
P 		129.8
(-0.3)
P 		192.7
(3)
P 		78.5
(-3)
P 		114
B 151.1
(1.1)
P 		176.9
(3)
P 		141.5
(0.4)
P 		96.5
(-3)
P 		80
C 175.2
(1.8)
P 		196.2
(3)
P 		168.2
(1.4)
P 		93.9
(-3)
P 		102
avr 172.7
(3) 		167.6
(2.6) 		167.5
(2.6) 		89.6
(-3) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 424
1 2 3 4 5 6 range
A 108.2
(-3)
P 		214.1
(-0.1)
P 		126.6
(-2.5)
P 		169.8
(-1.3)
P 		297
(2.3)
P 		323.8
(3)
P 		216
B 216.8
(-1.8)
P 		202.1
(-2)
P 		136.3
(-3)
P 		176.4
(-2.4)
P 		464.5
(2)
P 		532
(3)
P 		396
C 210.7
(-1.7)
P 		215.4
(-1.6)
P 		136.5
(-3)
P 		221.8
(-1.5)
P 		290.6
(-0.3)
P 		482.5
(3)
P 		346
avr 178.6
(-2.1) 		210.5
(-1.5) 		133.1
(-3) 		189.3
(-1.9) 		350.7
(1.2) 		446.1
(3) 		313
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190833_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7859
Sequence Type Exemplar sequence
Representative Public ID F35G12.11 NCBI
Target Description F35G12.11 /REP_DB=WormBase Gene ID /WP=CE00969 /TR=Q20057 /GB=CAA86333.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Enhancer of rudimentary
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065535(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065535 NCBI Caenorhabditis elegans F35G12.11 (F35G12.11) mRNA, complete cds. 11/11 None
SNAP00000042244 ENSEMBL cdna:SNAP chromosome:CEL140:III:4593198:4593630:1 10/11 None
GENEFINDER00000042255 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4593198:4593630:1 10/11 None
F35G12.11.1 ENSEMBL cdna:known chromosome:CEL140:III:4593141:4593777:1 gene:F35G12.11 11/11 None
F35G12.11.2 ENSEMBL cdna:known chromosome:CEL140:III:4593196:4593792:1 gene:F35G12.11 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4593112-4593630(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35G12.11
Entrez Gene 185323 Entrez gene
SwissProt Q20057 EMBL-EBI
WormBase CE36485 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86333 Hypothetical protein F35G12.11 [Caenorhabditis elegans] ref|NP_497936.2| F35G12.11 [Caenorhabditis elegans] 2.0E-60
blast CAE71176 Hypothetical protein CBG18033 [Caenorhabditis briggsae] 4.0E-55
Protein Domains
Database ID Description E-Value
Pfam IPR000781 EMBL-EBI Enhancer of rudimentary 3.0E-35
Sequence
Target Sequence 
>C. ELEGANS:190833_AT
tatgcatatatgtctacttcttcacataccgtacttctgattcaaacatcaccacgtctt
gattcacggacttggggagattatgagagtgtaactgacgctcttgatgctctttgcaag
atgtttgaagatttcctttccaagaaatcagctgctccagtcacatacgacgtgtcgcaa
gtctacgagtttctcgataagctctccgatgtttctatgatgatcttcaatcgcgaaacc
gggcaatacattggtcgtacacgtgcatggatcaaacaacaagtctacgagatgatgcgt
ggtcgttgtcagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGCATATATGTCTACTTCTTCAC 501 657 79 Antisense
TCTTCACATACCGTACTTCTGATTC 75 615 97 Antisense
ATCACCACGTCTTGATTCACGGACT 297 27 126 Antisense
GTAACTGACGCTCTTGATGCTCTTT 632 461 169 Antisense
GAAATCAGCTGCTCCAGTCACATAC 95 363 222 Antisense
GACGTGTCGCAAGTCTACGAGTTTC 587 375 247 Antisense
ACGAGTTTCTCGATAAGCTCTCCGA 644 93 263 Antisense
AGCTCTCCGATGTTTCTATGATGAT 114 81 278 Antisense
TGATCTTCAATCGCGAAACCGGGCA 676 559 299 Antisense
GGTCGTACACGTGCATGGATCAAAC 635 501 331 Antisense
GAGATGATGCGTGGTCGTTGTCAGC 124 397 367 Antisense
Sequence Source Affymetrix Proprietary Database