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[190827_at] Human HPRP18 protein like

Description F32B6.3 /REP_DB=WormBase Gene ID /WP=CE20741 /TR=O45431 /GB=CAB03039.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Human HPRP18 protein like
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 76      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 83      6grp_total > 66      4grp_total > 03      6grp_highest_sub > Da      6grp_high1_sub > Da      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 689
1 2 3 4 range
A 372.4
(-2.1)
P 		292.5
(-2.8)
P 		273.2
(-3)
P 		961.8
(3)
P 		689
B 330.9
(-2.6)
P 		318.2
(-2.7)
P 		286
(-3)
P 		890.5
(3)
P 		605
C 362.5
(-2.3)
P 		359.6
(-2.3)
P 		314.2
(-3)
P 		729.8
(3)
P 		416
avr 355.3
(-2.3) 		323.4
(-2.7) 		291.1
(-3) 		860.7
(3) 		570

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 516
1 2 3 4 5 6 range
A 346.3
(-3)
P 		359.2
(-2.5)
P 		476
(1.7)
P 		410.6
(-0.7)
P 		393.5
(-1.3)
P 		512
(3)
P 		166
B 400.7
(-3)
P 		418.8
(-2.6)
P 		511.5
(-0.8)
P 		441.8
(-2.2)
P 		410.2
(-2.8)
P 		702.2
(3)
P 		302
C 269.2
(-3)
P 		442.4
(-1)
P 		273
(-3)
P 		451.7
(-0.9)
P 		293.7
(-2.7)
P 		785.4
(3)
P 		516
avr 338.7
(-3) 		406.8
(-1.8) 		420.2
(-1.5) 		434.7
(-1.2) 		365.8
(-2.5) 		666.5
(3) 		328
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 135.6
(2.6)
P 		63.5
(-3)
P 		71.4
(-2.4)
P 		140.8
(3)
P 		77
B 105.5
(-1.1)
P 		78.6
(-2.4)
P 		66.3
(-3)
P 		189.8
(3)
P 		124
C 120
(2.1)
P 		103.4
(-3)
P 		109.5
(-1.1)
P 		123.1
(3)
P 		20
avr 120.4
(0.3) 		81.8
(-3) 		82.4
(-2.9) 		151.2
(3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 199
1 2 3 4 5 6 range
A 63.8
(-3)
P 		127.8
(3)
P 		103.7
(0.7)
P 		104.1
(0.8)
P 		118.7
(2.1)
P 		124.7
(2.7)
P 		64
B 139.4
(-2.1)
P 		139.4
(-2.1)
P 		124.2
(-2.7)
P 		117
(-3)
P 		166.6
(-0.9)
P 		261.8
(3)
P 		145
C 99.8
(-2.1)
P 		135
(-1)
P 		69.5
(-3)
P 		90.8
(-2.3)
P 		95
(-2.2)
P 		262.9
(3)
P 		193
avr 101
(-2.9) 		134.1
(-1.2) 		99.1
(-3) 		104
(-2.7) 		126.8
(-1.6) 		216.5
(3) 		117
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190827_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9042
Sequence Type Exemplar sequence
Representative Public ID F32B6.3 NCBI
Target Description F32B6.3 /REP_DB=WormBase Gene ID /WP=CE20741 /TR=O45431 /GB=CAB03039.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Human HPRP18 protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069375(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069375 NCBI Caenorhabditis elegans F32B6.3 (F32B6.3) mRNA, complete cds. 10/11 None
SNAP00000041528 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9882173:9884253:-1 10/11 None
GENEFINDER00000041541 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9882173:9884253:-1 10/11 None
F32B6.3 ENSEMBL cdna:known chromosome:CEL140:IV:9882107:9884253:-1 gene:F32B6.3 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9882178-9884259(-) 100.0 100.0
Public Domain and Genome References
Gene Title Human HPRP18 protein like
Ensembl F32B6.3
Entrez Gene 177840 Entrez gene
SwissProt O45431 EMBL-EBI
WormBase CE35518 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:263116_S_AT splicing factor Prp18 family protein at
ATGENOME1:12008_AT splicing factor Prp18 family protein at
CANINE_2:CFA.17745.1.S1_S_AT similar to Pre-mRNA splicing factor 18 (PRP18 homolog) (hPRP18) cfa
CANINE_2:CFAAFFX.8030.1.S1_AT similar to Pre-mRNA splicing factor 18 (PRP18 homolog) (hPRP18) cfa
DROSOPHILA_2:1624848_AT dm
DROSGENOME1:153988_AT dm
CHICKEN:GGA.5605.1.S1_A_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) gga
HG-U133_PLUS_2:223685_S_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HG-U133B:223685_S_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HG-U133A_2:221547_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HG-U133A:221547_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HG-U133_PLUS_2:221547_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HG-FOCUS:221547_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HU35KSUBA:U51990_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HUGENEFL:U51990_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HU35KSUBB:RC_H82227_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
U133_X3P:G12803490_3P_S_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
U133_X3P:G12653992_3P_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HG-U95AV2:40269_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HG-U95B:51840_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HG-U133A_2:221546_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HG-U133_PLUS_2:221546_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
HG-U133A:221546_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) hs
MG-U74BV2:116358_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) mm
MG-U74BV2:162921_I_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) mm
MOE430B:1429614_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) mm
MOUSE430_2:1429614_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) mm
MU19KSUBC:TC41842_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) (Prpf18), mRNA mm
RAE230A:1388005_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) rn
RAT230_2:1388005_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) rn
RG-U34B:RC_AI029421_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) rn
RAE230A:1399021_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) rn
RAT230_2:1399021_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) rn
RAE230B:1398014_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) rn
RAT230_2:1398014_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) rn
RG-U34B:RC_AI060318_AT PRP18 pre-mRNA processing factor 18 homolog (yeast) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8380 RNA splicing inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5681 spliceosome complex inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03039 Hypothetical protein F32B6.3 [Caenorhabditis elegans] ref|NP_501776.2| F32B6.3 [Caenorhabditis elegans] 0.0
blast CAE59974 Hypothetical protein CBG03465 [Caenorhabditis briggsae] 1.0E-176
blast CAB03039 Hypothetical protein F32B6.3 [Caenorhabditis elegans] ref|NP_501776.2| F32B6.3 [Caenorhabditis elegans] 1.0E-149
blast CAE59974 Hypothetical protein CBG03465 [Caenorhabditis briggsae] 1.0E-124
blast XP_707528 PREDICTED: similar to PRP18 pre-mRNA processing factor 18 homolog isoform 2 [Danio rerio] 5.0E-64
Protein Domains
Database ID Description E-Value
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 6.69999980926514
Pfam IPR004098 EMBL-EBI Prp18 domain 5.7E-100
Pfam IPR004098 EMBL-EBI Prp18 domain 1.8E-32
Pfam IPR004098 EMBL-EBI Prp18 domain 3.0E-60
Sequence
Target Sequence 
>C. ELEGANS:190827_AT
ggtacagctgatagtgcaacacgacatgatattgctcttccacaaggatatgaagaagat
aattggaaaaatatcgaacataattcaactcttttgagcgtcgatgacgatttgaaacga
gattgtgatattattctttcaatttgccgttatatactcgcaagatgggctaaggatctg
aacgataggccactggatgtaaaaaagacagcacaagcagcccatcacaagcaaacaatg
atgcatcttaaaagtttaatgacttcaatggaaagatacaactgtaataatgatattcgg
catcatttggccaagatttgtagattgctagtaattgatagaaattatttggaagcaaat
aatgcctatatggaaatggccatcggaaatgcaccttggccagttggagtaactcgttct
ggtattcatcaacgtcctggatctgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTACAGCTGATAGTGCAACACGAC 271 505 463 Antisense
CACGACATGATATTGCTCTTCCACA 42 201 482 Antisense
TCAACTCTTTTGAGCGTCGATGACG 255 631 547 Antisense
GATATTATTCTTTCAATTTGCCGTT 392 427 589 Antisense
GGATCTGAACGATAGGCCACTGGAT 362 511 636 Antisense
AAAAGACAGCACAAGCAGCCCATCA 550 131 665 Antisense
GATATTCGGCATCATTTGGCCAAGA 470 427 754 Antisense
GAAATGGCCATCGGAAATGCACCTT 420 361 835 Antisense
GAAATGCACCTTGGCCAGTTGGAGT 236 359 848 Antisense
GAGTAACTCGTTCTGGTATTCATCA 618 397 869 Antisense
TATTCATCAACGTCCTGGATCTGCA 25 663 885 Antisense
Sequence Source Affymetrix Proprietary Database