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[190820_s_at] Man(9)-alpha-mannosidase

Description ZC410.3 /REP_DB=WormBase Gene ID /WP=CE03807 /TR=Q23296 /GB=CAA92567.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Man(9)-alpha-mannosidase
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 87      4grp_highest_8_8 > 62      4grp_high_8_8 > 18      6grp_highest_nonorm > 92      6grp_total > 87      4grp_total > 29      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 130.3
(3)
P 		79.2
(-1.7)
P 		64.4
(-3)
P 		95.3
(-0.2)
P 		66
B 101.9
(-0.6)
P 		118.4
(0.6)
P 		69.3
(-3)
P 		150.1
(3)
P 		81
C 157.6
(3)
P 		116.7
(-1.4)
P 		102.4
(-3)
P 		141.7
(1.3)
P 		55
avr 129.9
(3) 		104.8
(0.1) 		78.7
(-3) 		129
(2.9) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 668
1 2 3 4 5 6 range
A 90.7
(-3)
P 		100.1
(-2.9)
P 		149.9
(-2.3)
P 		171.6
(-2.1)
P 		347.1
(-0.2)
P 		635.1
(3)
P 		544
B 69.3
(-3)
P 		112.7
(-2.6)
P 		105.7
(-2.6)
P 		208.8
(-1.6)
P 		332
(-0.4)
P 		673.3
(3)
P 		604
C 69.6
(-3)
P 		102.2
(-2.7)
P 		144.2
(-2.3)
P 		168.2
(-2.1)
P 		316
(-0.8)
P 		737.2
(3)
P 		668
avr 76.5
(-3) 		105
(-2.7) 		133.3
(-2.4) 		182.9
(-1.9) 		331.7
(-0.5) 		681.9
(3) 		605
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 47.4
(3)
P 		17.2
(-2.4)
P 		16.8
(-2.5)
P 		13.9
(-3)
P 		34
B 32.5
(3)
P 		29.2
(1.8)
P 		16.1
(-3)
P 		32
(2.8)
P 		16
C 52.2
(3)
P 		33.6
(-0.9)
P 		35.7
(-0.5)
P 		23.9
(-3)
P 		28
avr 44
(3) 		26.7
(-1.9) 		22.9
(-3) 		23.3
(-2.9) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 234
1 2 3 4 5 6 range
A 16.7
(-3)
P 		35.6
(-2.2)
P 		32.7
(-2.3)
P 		43.5
(-1.8)
P 		104.8
(0.8)
P 		154.6
(3)
P 		138
B 24.1
(-3)
P 		37.5
(-2.6)
P 		25.7
(-3)
P 		55.3
(-2.2)
P 		134.9
(-0.1)
P 		251.1
(3)
P 		227
C 25.8
(-3)
P 		31.2
(-2.9)
P 		36.7
(-2.7)
P 		33.8
(-2.8)
P 		102.2
(-0.9)
P 		246.8
(3)
P 		221
avr 22.2
(-3) 		34.8
(-2.6) 		31.7
(-2.7) 		44.2
(-2.3) 		114
(-0.2) 		217.5
(3) 		195
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190820_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10030
Sequence Type Exemplar sequence
Representative Public ID ZC410.3 NCBI
Target Description ZC410.3 /REP_DB=WormBase Gene ID /WP=CE03807 /TR=Q23296 /GB=CAA92567.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Man(9)-alpha-mannosidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069176(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069176 NCBI Caenorhabditis elegans ZC410.3 (Man(9)-alpha-mannosidase) mRNA, complete cds. 11/11 None
SNAP00000030924 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9076574:9078361:-1 11/11 None
GENEFINDER00000030941 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9076574:9078463:-1 11/11 None
ZC410.3 ENSEMBL cdna:known chromosome:CEL140:IV:9076503:9078577:-1 gene:ZC410.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9076579-9078469(-) 100.0 100.0
Public Domain and Genome References
Gene Title Man(9)-alpha-mannosidase
Ensembl ZC410.3
Entrez Gene 177726 Entrez gene
SwissProt Q23296 EMBL-EBI
WormBase CE03807 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6487 protein amino acid N-linked glycosylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92567 Hypothetical protein ZC410.3 [Caenorhabditis elegans] ref|NP_501577.1| ZC410.3 [Caenorhabditis elegans] 0.0
blast CAE74098 Hypothetical protein CBG21758 [Caenorhabditis briggsae] 0.0
blast NP_508877 T03G11.4 [Caenorhabditis elegans] gb|AAA82446.2| Hypothetical protein T03G11.4 [Caenorhabditis elegans] 1.0E-157
Protein Domains
Database ID Description E-Value
Pfam IPR001382 EMBL-EBI Glycoside hydrolase, family 47 1.0E-126
Pfam IPR001382 EMBL-EBI Glycoside hydrolase, family 47 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501577.1 1 12-34
ZC410.3 1 12-34
GENEFINDER00000030941 1 12-34
Sequence
Target Sequence 
>C. ELEGANS:190820_S_AT
gcatctctatagacaatctcagccgaatagcatctggtttctcggtgaaataaccgaatt
ggcacagttctatccaaaaatggatcaccttgtctgtttcctttccggatctcttgtact
ttcccatttaaatggtcttgatcatgacaatgaacatttggaaatggctaaaaatattgg
aaatgtttgtcataaaatgtatgaaaatccaacaggacttggaccagaaataatacattt
caatatggaggatagttctgatatgacacaagcggatacttatgtgaagaatcttgatgc
tcattcacttctccgtccagaagcaattgaagcgtggttctatttgtatcgtgttactaa
agataagaaatatcaagagtggggatggaaagcttttgagtcgattgaaaagtatgcaaa
agtggaaactggtggatacagctcaattgacaatgtgctcagaaagaagatcaaaagacg
tgacaaaatggaatcattcttccttgctgagaccctcaaatacctttaccttctaatggc
tgatgatcaagaaatccttccactcgatcgatgggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATCTCTATAGACAATCTCAGCCG 350 309 996 Antisense
AATAGCATCTGGTTTCTCGGTGAAA 633 171 1021 Antisense
TAACCGAATTGGCACAGTTCTATCC 554 637 1046 Antisense
AATGGATCACCTTGTCTGTTTCCTT 176 163 1074 Antisense
ATCTCTTGTACTTTCCCATTTAAAT 262 31 1104 Antisense
GAATCTTGATGCTCATTCACTTCTC 127 329 1284 Antisense
CATTCACTTCTCCGTCCAGAAGCAA 158 217 1297 Antisense
GAAGCGTGGTTCTATTTGTATCGTG 539 343 1324 Antisense
GGAATCATTCTTCCTTGCTGAGACC 573 533 1485 Antisense
ATACCTTTACCTTCTAATGGCTGAT 609 21 1515 Antisense
AAATCCTTCCACTCGATCGATGGGT 197 119 1547 Antisense
Sequence Source Affymetrix Proprietary Database