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[190814_s_at] serine/threonine kinase

Description C50F4.10 /REP_DB=WormBase Gene ID /WP=CE05474 /TR=Q18738 /GB=CAA94735.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 78      4grp_total > 03      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 41.4
(0.5)
A 		4.2
(-3)
A 		8.1
(-2.6)
A 		68.8
(3)
A 		65
B 7.3
(-3)
A 		24.9
(-0.9)
A 		56.3
(2.8)
A 		58.1
(3)
A 		51
C 44.4
(-1)
A 		42.5
(-1.1)
A 		19.2
(-3)
A 		93.2
(3)
A 		74
avr 31
(-2.1) 		23.9
(-3) 		27.9
(-2.5) 		73.4
(3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 50.1
(-1.2)
A 		12.3
(-2.9)
A 		20.6
(-2.5)
A 		9.7
(-3)
A 		87.7
(0.4)
P 		148
(3)
P 		138
B 18.9
(-2.4)
A 		25.1
(-2.2)
A 		4.4
(-3)
A 		18.4
(-2.5)
A 		113.4
(1.2)
P 		158.4
(3)
P 		154
C 33.1
(-1.9)
A 		54
(-1.2)
A 		2.9
(-3)
A 		10.7
(-2.7)
A 		63
(-0.8)
P 		170.4
(3)
P 		168
avr 34
(-2) 		30.5
(-2.1) 		9.3
(-3) 		12.9
(-2.9) 		88
(0.2) 		158.9
(3) 		150
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 15.1
(3)
A 		0.9
(-3)
A 		2.1
(-2.5)
A 		10.1
(0.9)
A 		14
B 2.3
(-3)
A 		6.1
(-0.9)
A 		13.1
(3)
A 		12.4
(2.6)
A 		11
C 14.7
(2.3)
A 		12.2
(0.7)
A 		6.7
(-3)
A 		15.7
(3)
A 		9
avr 10.7
(1.1) 		6.4
(-3) 		7.3
(-2.1) 		12.7
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 9.2
(-1.8)
A 		4.4
(-2.7)
A 		4.5
(-2.6)
A 		2.5
(-3)
A 		26.5
(1.3)
P 		36
(3)
P 		34
B 6.6
(-2.4)
A 		8.4
(-2.2)
A 		1.1
(-3)
A 		4.9
(-2.6)
A 		46.1
(1.7)
P 		59.1
(3)
P 		58
C 12.3
(-1.8)
A 		16.5
(-1.3)
A 		0.7
(-3)
A 		2.2
(-2.8)
A 		20.4
(-0.9)
P 		57
(3)
P 		56
avr 9.4
(-2.1) 		9.8
(-2) 		2.1
(-3) 		3.2
(-2.9) 		31
(0.6) 		50.7
(3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190814_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11649
Sequence Type Exemplar sequence
Representative Public ID C50F4.10 NCBI
Target Description C50F4.10 /REP_DB=WormBase Gene ID /WP=CE05474 /TR=Q18738 /GB=CAA94735.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073055(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073055 NCBI Caenorhabditis elegans C50F4.10 (C50F4.10) mRNA, complete cds. 11/11 None
SNAP00000009309 ENSEMBL cdna:SNAP chromosome:CEL140:V:9532634:9534084:-1 11/11 None
GENEFINDER00000009327 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9532634:9534084:-1 11/11 None
C50F4.10 ENSEMBL cdna:known chromosome:CEL140:V:9532634:9534084:-1 gene:C50F4.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000005152 1/11 Cross Hyb Matching Probes None
K08H10.5 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9532633-9534084(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase
Ensembl C50F4.10
Entrez Gene 179334 Entrez gene
SwissProt Q18738 EMBL-EBI
WormBase CE33984 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94735 Hypothetical protein C50F4.10 [Caenorhabditis elegans] ref|NP_505456.2| C50F4.10 [Caenorhabditis elegans] 0.0
blast CAE72300 Hypothetical protein CBG19430 [Caenorhabditis briggsae] 1.0E-158
blast CAE72300 Hypothetical protein CBG19430 [Caenorhabditis briggsae] 1.0E-157
blast AAK85494 Hypothetical protein R13H9.5 [Caenorhabditis elegans] ref|NP_500715.1| R13H9.5 [Caenorhabditis elegans] 4.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 9.7E-13
Pfam IPR000719 EMBL-EBI Protein kinase 9.7E-13
Sequence
Target Sequence 
>C. ELEGANS:190814_S_AT
gagcaatctcagtcaattgtgccctgagcgtcggaattcaactcatcgaagcactcgaag
atcttcacaattgtggcttcattcatcgagacctgaaaccttcaaatatctgttttggac
ggaaagatcgtaacgaacttggaaagttgtatcttttgagttttggaatttcacgacgtt
acttggactccaaaaatcaaatgagaaaacctcgtgacaatgttgaattccgtggaaccg
ttcgatatgcttcattgaattgtcatcaactgcaagaactgaatagaaaagatgatttag
aatcagctttctacgttatcgttgaaatgatcactggacaacttccatggaaaggtcttc
cagatggtgctgcagttgcacgagtcaagcaacaaagtcgtcacccatcagcaatcgctc
ttttcttgagagcaccttgtccgatggaagaacttcgtgaagtgatgattctcattgatt
cctacaattatttcacagctcccgattatgttaatatctgcaacattctccgtcgggcaa
tgcgcagaagtcctcaaccggaacatcctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCAATCTCAGTCAATTGTGCCCT 399 391 398 Antisense
TGCCCTGAGCGTCGGAATTCAACTC 519 583 417 Antisense
ATTTCACGACGTTACTTGGACTCCA 73 17 565 Antisense
GAATCAGCTTTCTACGTTATCGTTG 615 327 697 Antisense
AGGTCTTCCAGATGGTGCTGCAGTT 20 59 750 Antisense
GTCAAGCAACAAAGTCGTCACCCAT 669 463 781 Antisense
CCATCAGCAATCGCTCTTTTCTTGA 355 271 802 Antisense
TCTTGAGAGCACCTTGTCCGATGGA 313 619 821 Antisense
CCTACAATTATTTCACAGCTCCCGA 671 267 878 Antisense
TCTCCGTCGGGCAATGCGCAGAAGT 311 609 924 Antisense
AGAAGTCCTCAACCGGAACATCCTT 124 73 943 Antisense
Sequence Source Affymetrix Proprietary Database