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[190810_at] Diacylglycerol kinase

Description F54G8.2 /REP_DB=WormBase Gene ID /WP=CE00204 /TR=SW:Q03603 /GB=CAA79558.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Diacylglycerol kinase
Cluster 6grp_total > 11      4grp_total > 21      6grp_cgc > 44      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 153
1 2 3 4 range
A 67.7
(3)
A 		21.4
(-3)
A 		23.2
(-2.8)
A 		34.9
(-1.3)
A 		46
B 73.2
(1.1)
A 		47.7
(-0.7)
A 		12.8
(-3)
A 		102.1
(3)
A 		89
C 89.6
(0.1)
A 		59.8
(-1.1)
A 		11.3
(-3)
A 		164.7
(3)
M 		153
avr 76.8
(1.3) 		43
(-1.1) 		15.8
(-3) 		100.6
(3) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 53.9
(-1.4)
A 		72.9
(-0.1)
A 		114.8
(3)
A 		77
(0.2)
A 		32.5
(-3)
A 		76.9
(0.2)
A 		82
B 67.4
(1.7)
A 		84.5
(3)
A 		52.6
(0.5)
A 		8.3
(-3)
A 		33.2
(-1)
A 		28.6
(-1.4)
A 		76
C 36.9
(-1.5)
A 		101.5
(3)
A 		44
(-1)
A 		46.6
(-0.8)
A 		15.4
(-3)
A 		39.9
(-1.3)
A 		86
avr 52.7
(-0.4) 		86.3
(3) 		70.5
(1.4) 		44
(-1.3) 		27
(-3) 		48.5
(-0.8) 		59
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 24.7
(3)
A 		4.6
(-3)
A 		6.1
(-2.6)
A 		5.1
(-2.9)
A 		20
B 23.3
(3)
A 		11.8
(-0.4)
A 		3
(-3)
A 		21.8
(2.6)
A 		20
C 29.7
(3)
A 		17.2
(0.1)
A 		3.9
(-3)
A 		27.8
(2.6)
M 		26
avr 25.9
(3) 		11.2
(-1.1) 		4.3
(-3) 		18.2
(0.9) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 9.9
(-3)
A 		25.9
(3)
A 		25
(2.7)
A 		19.5
(0.6)
A 		9.8
(-3)
A 		18.7
(0.3)
A 		16
B 23.5
(1.9)
A 		28.1
(3)
A 		12.8
(-0.5)
A 		2.2
(-3)
A 		13.5
(-0.4)
A 		10.7
(-1)
A 		26
C 13.7
(-1)
A 		31
(3)
A 		11.2
(-1.6)
A 		9.4
(-2)
A 		5
(-3)
A 		13.4
(-1.1)
A 		26
avr 15.7
(-1) 		28.3
(3) 		16.3
(-0.8) 		10.4
(-2.7) 		9.4
(-3) 		14.3
(-1.4) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190810_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6513
Sequence Type Exemplar sequence
Representative Public ID F54G8.2 NCBI
Target Description F54G8.2 /REP_DB=WormBase Gene ID /WP=CE00204 /TR=SW:Q03603 /GB=CAA79558.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Diacylglycerol kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066630(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066630 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-3) (dgk-3) mRNA, complete cds. 11/11 None
F54G8.2 ENSEMBL cdna:known chromosome:CEL140:III:9162982:9167932:1 gene:F54G8.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000023862 8/11 Cross Hyb Matching Probes None
GENEFINDER00000023864 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9162977-9167928(+) 100.0 100.0
Public Domain and Genome References
Gene Title Diacylglycerol kinase
Gene Symbol dgk-3
Ensembl F54G8.2
Entrez Gene 186262 Entrez gene
SwissProt Q03603 EMBL-EBI
WormBase CE33654 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.4466.1.S1_S_AT similar to Diacylglycerol kinase, beta (Diglyceride kinase) (DGK-beta) (DAG kinase beta) (90 kDa diacylglycerol kinase) cfa
CHICKEN:GGAAFFX.3579.2.S1_S_AT similar to Diacylglycerol kinase, beta (Diglyceride kinase) (DGK-beta) (DAG kinase beta) (90 kDa diacylglycerol kinase) gga
HG-U95AV2:31400_AT diacylglycerol kinase, beta 90kDa hs
U133_X3P:HS2.158318.2.S1_3P_AT diacylglycerol kinase, beta 90kDa hs
U133_X3P:HS.158318.0.S1_3P_AT diacylglycerol kinase, beta 90kDa hs
HG-U133_PLUS_2:1553524_AT diacylglycerol kinase, beta 90kDa hs
HG-U133A_2:216307_AT diacylglycerol kinase, beta 90kDa hs
HG-U133_PLUS_2:216307_AT diacylglycerol kinase, beta 90kDa hs
HG-U133A:216307_AT diacylglycerol kinase, beta 90kDa hs
MOUSE430_2:1455361_AT diacylglycerol kinase, beta mm
MOE430B:1455361_AT diacylglycerol kinase, beta mm
MG-U74CV2:135245_AT diacylglycerol kinase, beta mm
MOE430B:1440901_AT diacylglycerol kinase, beta mm
MOUSE430_2:1440901_AT diacylglycerol kinase, beta mm
MOE430B:1442860_AT diacylglycerol kinase, beta mm
MOUSE430_2:1442860_AT diacylglycerol kinase, beta mm
RG-U34A:RC_AA818983_AT hypothetical gene supported by NM_019304 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7205 protein kinase C activation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4143 diacylglycerol kinase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA79558 Hypothetical protein F54G8.2 [Caenorhabditis elegans] ref|NP_499031.2| DiacylGlycerol Kinase family member (dgk-3) [Caenorhabditis elegans] sp|Q03603|DGK3_CAEEL Probable diacylglycerol kinase 3 (Diglyceride kinase 3) (DGK 3) (DAG kinase 3) 0.0
blast CAE62666 Hypothetical protein CBG06804 [Caenorhabditis briggsae] 0.0
blast BAE23997.1 unnamed protein product [Mus musculus] gb|AAH70461.1| Dgkb protein [Mus musculus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 1.7E-66
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.9E-4
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 8.3E-4
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 8.1E-21
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 1.4E-11
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 2.0E-119
Sequence
Target Sequence 
>C. ELEGANS:190810_AT
gtgagaccctatcaagttcttgcaagaacttacacgagcaaattgatattttgtgcgatg
gagaatcaatcgatcttggacaagatgcatcacttgaaggaattgctcttttgaatattc
catcaatttacggaggatctaatttatggggaaggagtcgaaaatctaaaggaagaatgc
ctgggctgttcccaatgaaaaatgccgagaaaatgcaattacagacaagagttcaagata
ttggagatggtctcattgagttagttggattggagtcagcaatgcaaatgggacagatta
aagcgggtgtacgtggagcaagacggctttctcaatgttcaactgttgtgattcaaacac
acaaatcatttcccatgcaaattgacggggaaccatggatgcaacctccttgcatcattc
aaatcacacacaaaaaccaagcaaaaatgcttgttggtgagtttcgtcatatcctcgagt
tgtcgattaatttctttttattcacagctgcagctccacgaaaacgaagctcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGAGACCCTATCAAGTTCTTGCAA 585 481 1922 Antisense
GAAGAATGCCTGGGCTGTTCCCAAT 299 339 2093 Antisense
GTACGTGGAGCAAGACGGCTTTCTC 163 459 2230 Antisense
GACGGCTTTCTCAATGTTCAACTGT 114 377 2243 Antisense
AACACACAAATCATTTCCCATGCAA 280 135 2277 Antisense
GACGGGGAACCATGGATGCAACCTC 75 377 2305 Antisense
GCAACCTCCTTGCATCATTCAAATC 593 319 2322 Antisense
AATGCTTGTTGGTGAGTTTCGTCAT 43 167 2367 Antisense
GTTTCGTCATATCCTCGAGTTGTCG 248 451 2382 Antisense
TTCTTTTTATTCACAGCTGCAGCTC 178 691 2413 Antisense
GCAGCTCCACGAAAACGAAGCTCCT 449 313 2431 Antisense
Sequence Source Affymetrix Proprietary Database