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[190791_at] Initiation factor 5A

Description T05G5.10 /REP_DB=WormBase Gene ID /WP=CE00503 /TR=SW:P34563 /GB=CAA81597.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Initiation factor 5A
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 98      4grp_highest_8_8 > 14      4grp_high_8_8 > 15      6grp_highest_nonorm > 39      6grp_total > 95      4grp_total > 08      6grp_highest_sub > In      6grp_cgc > 78      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20256
1 2 3 4 range
A 902.8
(-3)
P 		3114.8
(-2.3)
P 		6472.1
(-1.3)
P 		21140.8
(3)
P 		20238
B 884.4
(-3)
P 		3199.2
(-2.2)
P 		6382.9
(-1.1)
P 		18174.6
(3)
P 		17290
C 986.2
(-3)
P 		3209.7
(-2.3)
P 		5864.9
(-1.5)
P 		20251.7
(3)
P 		19266
avr 924.5
(-3) 		3174.6
(-2.3) 		6240
(-1.3) 		19855.7
(3) 		18931

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12680
1 2 3 4 5 6 range
A 720
(-3)
P 		1187.4
(-2.8)
P 		3924.2
(-1.4)
P 		7429.1
(0.4)
P 		10810.9
(2.1)
P 		12549.7
(3)
P 		11830
B 688.2
(-3)
P 		1321.5
(-2.7)
P 		3823.2
(-1.4)
P 		7484.4
(0.5)
P 		11894
(2.7)
P 		12473.7
(3)
P 		11786
C 605.5
(-3)
P 		1278.7
(-2.7)
P 		2881.1
(-1.9)
P 		8816.4
(0.9)
P 		10940.3
(1.9)
P 		13285.2
(3)
P 		12680
avr 671.2
(-3) 		1262.5
(-2.7) 		3542.8
(-1.6) 		7910
(0.6) 		11215.1
(2.2) 		12769.5
(3) 		12098
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3591
1 2 3 4 range
A 328.7
(-3)
P 		676.1
(-2.2)
P 		1691.9
(-0)
P 		3094.6
(3)
P 		2766
B 282
(-3)
P 		790.1
(-2.2)
P 		1479.1
(-1)
P 		3873
(3)
P 		3591
C 326.5
(-3)
P 		922.8
(-1.8)
P 		2044.1
(0.3)
P 		3414.8
(3)
P 		3088
avr 312.4
(-3) 		796.3
(-2.1) 		1738.4
(-0.3) 		3460.8
(3) 		3148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4700
1 2 3 4 5 6 range
A 132.7
(-3)
P 		422.3
(-2.4)
P 		854.6
(-1.6)
P 		1884.2
(0.4)
P 		3262.3
(3)
P 		3055.5
(2.6)
P 		3130
B 239.5
(-3)
P 		439.9
(-2.7)
P 		928.4
(-2.1)
P 		1982.4
(-0.7)
P 		4832.4
(3)
P 		4651.4
(2.8)
P 		4593
C 224.4
(-3)
P 		390.2
(-2.8)
P 		733.3
(-2.3)
P 		1773
(-0.8)
P 		3540.1
(1.7)
P 		4447.3
(3)
P 		4223
avr 198.9
(-3) 		417.5
(-2.7) 		838.8
(-2) 		1879.9
(-0.4) 		3878.3
(2.7) 		4051.4
(3) 		3853
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190791_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7476
Sequence Type Exemplar sequence
Representative Public ID T05G5.10 NCBI
Target Description T05G5.10 /REP_DB=WormBase Gene ID /WP=CE00503 /TR=SW:P34563 /GB=CAA81597.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Initiation factor 5A
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066751(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066751 NCBI Caenorhabditis elegans Initiation Factor Five (eIF-5A) homologs family member (iff-1) (iff-1) mRNA, complete cds. 11/11 None
SNAP00000019876 ENSEMBL cdna:SNAP chromosome:CEL140:III:9745803:9746499:-1 11/11 None
GENEFINDER00000019890 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9745803:9746499:-1 11/11 None
T05G5.10 ENSEMBL cdna:known chromosome:CEL140:III:9745759:9747048:-1 gene:T05G5.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9745798-9746495(-) 100.0 100.0
Public Domain and Genome References
Gene Title Initiation factor 5A
Gene Symbol iff-1
Ensembl T05G5.10
Entrez Gene 176373 Entrez gene
SwissProt P34563 EMBL-EBI
WormBase CE37787 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261270_AT eukaryotic translation initiation factor 5A, putative / eIF-5A, putative at
CANINE:1587210_AT similar to eukaryotic translation initiation factor 5A cfa
CANINE_2:CFA.8657.1.A1_AT similar to eukaryotic translation initiation factor 5A cfa
CANINE_2:CFA.8657.1.A1_S_AT similar to eukaryotic translation initiation factor 5A cfa
CANINE_2:CFAAFFX.24750.1.S1_AT similar to eukaryotic translation initiation factor 5A cfa
DROSOPHILA_2:1638349_S_AT dm
DROSGENOME1:147834_AT dm
HG-U133_PLUS_2:201122_X_AT eukaryotic translation initiation factor 5A hs
HG-U133A:201122_X_AT eukaryotic translation initiation factor 5A hs
HG-U133A_2:201122_X_AT eukaryotic translation initiation factor 5A hs
HG-U95AV2:847_AT eukaryotic translation initiation factor 5A hs
HC-G110:847_AT eukaryotic translation initiation factor 5A hs
HG-U133_PLUS_2:201123_S_AT eukaryotic translation initiation factor 5A hs
HG-U133A:201123_S_AT eukaryotic translation initiation factor 5A hs
HG-U133A_2:201123_S_AT eukaryotic translation initiation factor 5A hs
HUGENEFL:U17969_AT eukaryotic translation initiation factor 5A hs
HUGENEFL:S72024_S_AT eukaryotic translation initiation factor 5A hs
U133_X3P:G4503544_3P_A_AT eukaryotic translation initiation factor 5A hs
U133_X3P:HS.119140.1.S1_3P_X_AT eukaryotic translation initiation factor 5A hs
U133_X3P:213753_3P_X_AT eukaryotic translation initiation factor 5A hs
HG-U133A_2:213753_X_AT eukaryotic translation initiation factor 5A hs
HG-U133_PLUS_2:213753_X_AT eukaryotic translation initiation factor 5A hs
HG-U133A:213753_X_AT eukaryotic translation initiation factor 5A hs
HG-FOCUS:213757_AT Eukaryotic translation initiation factor 5A hs
HG-U133A_2:213757_AT Eukaryotic translation initiation factor 5A hs
HG-U133_PLUS_2:213757_AT Eukaryotic translation initiation factor 5A hs
HG-U133A:213757_AT Eukaryotic translation initiation factor 5A hs
HG-U95C:66617_AT Eukaryotic translation initiation factor 5A hs
HG-U95D:74070_AT Eukaryotic translation initiation factor 5A hs
U133_X3P:213757_3P_AT Eukaryotic translation initiation factor 5A hs
U133_X3P:HS.119140.1.A1_3P_AT Eukaryotic translation initiation factor 5A hs
U133_X3P:HS.119140.1.A1_3P_X_AT Eukaryotic translation initiation factor 5A hs
MU11KSUBB:MSA.16208.0_F_AT eukaryotic translation initiation factor 5A mm
MU11KSUBB:MSA.5247.0_S_AT eukaryotic translation initiation factor 5A mm
MU11KSUBB:MSA.42958.0_F_AT eukaryotic translation initiation factor 5A mm
MOE430A:1437859_X_AT eukaryotic translation initiation factor 5A mm
MOUSE430A_2:1437859_X_AT eukaryotic translation initiation factor 5A mm
MOUSE430_2:1437859_X_AT eukaryotic translation initiation factor 5A mm
MOE430A:1451470_S_AT eukaryotic translation initiation factor 5A mm
MOUSE430A_2:1451470_S_AT eukaryotic translation initiation factor 5A mm
MOUSE430_2:1451470_S_AT eukaryotic translation initiation factor 5A mm
MU19KSUBA:TC22601_AT Eukaryotic translation initiation factor 5A, mRNA (cDNA clone MGC:25474 IMAGE:4482804) mm
MU19KSUBA:TC22602_AT Eukaryotic translation initiation factor 5A, mRNA (cDNA clone MGC:25474 IMAGE:4482804) mm
MU19KSUBA:TC22603_F_AT Eukaryotic translation initiation factor 5A, mRNA (cDNA clone MGC:25474 IMAGE:4482804) mm
MU19KSUBB:TC32763_AT Eukaryotic translation initiation factor 5A, mRNA (cDNA clone MGC:25474 IMAGE:4482804) mm
MU19KSUBC:TC36275_AT Eukaryotic translation initiation factor 5A, mRNA (cDNA clone MGC:25474 IMAGE:4482804) mm
MU19KSUBC:TC36277_AT Eukaryotic translation initiation factor 5A, mRNA (cDNA clone MGC:25474 IMAGE:4482804) mm
MU19KSUBC:TC38494_AT Eukaryotic translation initiation factor 5A, mRNA (cDNA clone MGC:25474 IMAGE:4482804) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6413 translational initiation inferred from electronic annotation QuickGO AmiGO
6413 translational initiation inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5829 cytosol inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3743 translation initiation factor activity inferred from electronic annotation QuickGO AmiGO
3743 translation initiation factor activity inferred from sequence similarity QuickGO AmiGO
45182 translation regulator activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA81597 Hypothetical protein T05G5.10 [Caenorhabditis elegans] ref|NP_499152.2| Initiation Factor Five (eIF-5A) homologs family member (iff-1) [Caenorhabditis elegans] 1.0E-109
blast CAE65142 Hypothetical protein CBG10008 [Caenorhabditis briggsae] 3.0E-97
blast CAA81597 Hypothetical protein T05G5.10 [Caenorhabditis elegans] ref|NP_499152.2| Initiation Factor Five (eIF-5A) homologs family member (iff-1) [Caenorhabditis elegans] 3.0E-89
blast P34563 Eukaryotic translation initiation factor 5A-1 (eIF-5A-1) (Initiation factor five protein 1) 3.0E-89
blast P34563 Eukaryotic translation initiation factor 5A-1 (eIF-5A-1) (Initiation factor five protein 1) 6.0E-89
Protein Domains
Database ID Description E-Value
Pfam IPR001884 EMBL-EBI Eukaryotic initiation factor 5A hypusine (eIF-5A) 1.2E-40
Pfam IPR005824 EMBL-EBI KOW 8.1E-8
Pfam IPR001884 EMBL-EBI Eukaryotic initiation factor 5A hypusine (eIF-5A) 1.2E-40
Pfam IPR005824 EMBL-EBI KOW 8.1E-8
Sequence
Target Sequence 
>C. ELEGANS:190791_AT
gacgaggagcaattcgactctgccgagtccggagctgctgctactttcccgaagcaatgc
tcggctcttcgcaagaacgagcacgtcatgatccgaggaagaccatgcaagatcgtcgag
atgagcacctccaagactggaaagcacggacatgctaaggttcacatggttgccatcgat
attttcaccaccaagaagcttgaggatatctgcccatccacccacaacatggacgtccca
gtcgtcaagagacgcgagtacattctcatgtcgatcgaagacgggttctgctcgctcatg
gatccagaatcttgcgagctcaaagacgatctcaaaatgccagaaggagacttgggaaac
actatccgtgaggctctcgagaaagacgaaggaagcgtcttggttcaagtcgttgctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGAGGAGCAATTCGACTCTGCCG 504 379 31 Antisense
GCTGCTACTTTCCCGAAGCAATGCT 475 297 67 Antisense
GAACGAGCACGTCATGATCCGAGGA 324 347 105 Antisense
AGATCGTCGAGATGAGCACCTCCAA 451 67 140 Antisense
GGTTGCCATCGATATTTTCACCACC 270 507 198 Antisense
ACAACATGGACGTCCCAGTCGTCAA 630 111 254 Antisense
GACGCGAGTACATTCTCATGTCGAT 393 381 281 Antisense
TCGATCGAAGACGGGTTCTGCTCGC 699 601 301 Antisense
GTTCTGCTCGCTCATGGATCCAGAA 539 443 315 Antisense
GAAACACTATCCGTGAGGCTCTCGA 452 355 386 Antisense
GCGTCTTGGTTCAAGTCGTTGCTGC 664 291 425 Antisense
Sequence Source Affymetrix Proprietary Database