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[190772_s_at] Phosphorylase kinase

Description C14B9.8 /REP_DB=WormBase Gene ID /WP=CE26870 /TR=SW:P34335 /GB=AAA27954.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Phosphorylase kinase
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      4grp_high_8_8 > 40      6grp_highest_nonorm > 61      6grp_total > 34      4grp_total > 46      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 334
1 2 3 4 range
A 565.5
(3)
P 		363.5
(-0.9)
P 		250.8
(-3)
P 		281.7
(-2.4)
M 		315
B 477.5
(3)
P 		407.9
(1.3)
P 		354.9
(0)
P 		231.5
(-3)
P 		246
C 559
(3)
P 		273.1
(-3)
P 		348.7
(-1.4)
P 		336.2
(-1.7)
P 		286
avr 534
(3) 		348.2
(-1.4) 		318.1
(-2.2) 		283.1
(-3) 		251

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 460
1 2 3 4 5 6 range
A 468.4
(3)
P 		460
(2.7)
P 		445.7
(2.3)
P 		381
(0.2)
P 		283.6
(-2.9)
P 		281.2
(-3)
P 		187
B 594.7
(0.7)
P 		551.9
(0)
P 		740.9
(3)
P 		428.7
(-1.9)
P 		361.6
(-3)
P 		427.1
(-2)
P 		379
C 567.2
(3)
P 		563.6
(2.9)
P 		350.3
(-3)
P 		386.5
(-2)
P 		374.6
(-2.3)
P 		390.2
(-1.9)
P 		217
avr 543.4
(3) 		525.2
(2.5) 		512.3
(2.1) 		398.7
(-1.3) 		339.9
(-3) 		366.2
(-2.2) 		204
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 165
1 2 3 4 range
A 205.9
(3)
P 		78.9
(-1.6)
P 		65.6
(-2.1)
P 		41.2
(-3)
M 		165
B 152.3
(3)
P 		100.7
(-0)
P 		82.2
(-1.1)
P 		49.3
(-3)
P 		103
C 185
(3)
P 		78.5
(-2)
P 		121.5
(0)
P 		56.7
(-3)
P 		128
avr 181.1
(3) 		86
(-1.3) 		89.8
(-1.2) 		49.1
(-3) 		132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 86.3
(-1.9)
P 		163.6
(3)
P 		97.1
(-1.2)
P 		96.6
(-1.2)
P 		85.6
(-1.9)
P 		68.5
(-3)
P 		95
B 206.9
(3)
P 		183.7
(1.5)
P 		179.9
(1.3)
P 		113.5
(-3)
P 		146.9
(-0.9)
P 		159.3
(-0.1)
P 		93
C 210.2
(3)
P 		172
(1.3)
P 		89.2
(-2.5)
P 		77.7
(-3)
P 		121.2
(-1)
P 		130.6
(-0.6)
P 		133
avr 167.8
(2.6) 		173.1
(3) 		122.1
(-1) 		95.9
(-3) 		117.9
(-1.3) 		119.5
(-1.2) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190772_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6039
Sequence Type Exemplar sequence
Representative Public ID C14B9.8 NCBI
Target Description C14B9.8 /REP_DB=WormBase Gene ID /WP=CE26870 /TR=SW:P34335 /GB=AAA27954.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Phosphorylase kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066376(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066376 NCBI Caenorhabditis elegans C14B9.8 (C14B9.8) mRNA, complete cds. 11/11 None
SNAP00000018773 ENSEMBL cdna:SNAP chromosome:CEL140:III:8139831:8144369:-1 11/11 None
GENEFINDER00000018776 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8139831:8144550:-1 11/11 None
C14B9.8 ENSEMBL cdna:known chromosome:CEL140:III:8139546:8144534:-1 gene:C14B9.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8139826-8144713(-) 100.0 100.0
Public Domain and Genome References
Gene Title Phosphorylase kinase
Ensembl C14B9.8
Entrez Gene 176149 Entrez gene
SwissProt P34335 EMBL-EBI
WormBase CE38273 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605908_AT similar to Phosphorylase b kinase alpha regulatory chain, liver isoform (Phosphorylase kinase alpha L subunit) cfa
CANINE_2:CFA.5653.1.A1_AT similar to Phosphorylase b kinase alpha regulatory chain, liver isoform (Phosphorylase kinase alpha L subunit) cfa
CANINE_2:CFAAFFX.20064.1.S1_S_AT similar to Phosphorylase b kinase alpha regulatory chain, liver isoform (Phosphorylase kinase alpha L subunit) cfa
DROSOPHILA_2:1641396_A_AT dm
DROSGENOME1:142687_AT dm
CHICKEN:GGA.16730.1.S1_AT similar to Phosphorylase B kinase alpha regulatory chain, liver isoform (Phosphorylase kinase alpha L subunit) gga
CHICKEN:GGAAFFX.10567.1.S1_S_AT similar to Phosphorylase B kinase alpha regulatory chain, liver isoform (Phosphorylase kinase alpha L subunit) gga
HG-U133_PLUS_2:209439_S_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133A:209439_S_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133A_2:209439_S_AT phosphorylase kinase, alpha 2 (liver) hs
HUGENEFL:X80497_AT phosphorylase kinase, alpha 2 (liver) hs
U133_X3P:G1304117_3P_A_AT phosphorylase kinase, alpha 2 (liver) hs
U133_X3P:HS2.414737.1.S1_3P_S_AT phosphorylase kinase, alpha 2 (liver) hs
U133_X3P:HS2.233163.1.S1_3P_AT phosphorylase kinase, alpha 2 (liver) hs
U133_X3P:HS.54941.0.S2_3P_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133_PLUS_2:1559705_S_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133_PLUS_2:1560358_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133A_2:209438_AT phosphorylase kinase, alpha 2 (liver) hs
HG-FOCUS:209438_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133_PLUS_2:209438_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133A:209438_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U95AV2:36480_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U95D:67799_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U95E:73083_AT Phosphorylase kinase, alpha 2 (liver) hs
MOE430A:1434518_AT phosphorylase kinase alpha 2 mm
MOUSE430A_2:1434518_AT phosphorylase kinase alpha 2 mm
MOUSE430_2:1434518_AT phosphorylase kinase alpha 2 mm
MOE430A:1430492_AT phosphorylase kinase alpha 2 mm
MOUSE430A_2:1430492_AT phosphorylase kinase alpha 2 mm
MOUSE430_2:1430492_AT phosphorylase kinase alpha 2 mm
MU11KSUBB:MSA.34023.0_S_AT phosphorylase kinase alpha 2 mm
MU11KSUBB:MSA.31221.0_S_AT phosphorylase kinase alpha 2 mm
MG-U74AV2:104003_AT phosphorylase kinase alpha 2 mm
MOE430B:1437941_AT phosphorylase kinase alpha 2 mm
MOUSE430_2:1437941_AT phosphorylase kinase alpha 2 mm
MOE430B:1441882_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MOUSE430_2:1441882_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MOE430B:1442147_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MOUSE430_2:1442147_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MOE430B:1443402_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MOUSE430_2:1443402_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MU19KSUBB:TC32673_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
Protein Similarities
Method ID Description E-Value
blast AAA27954 Hypothetical protein C14B9.8 [Caenorhabditis elegans] ref|NP_498777.2| C14B9.8 [Caenorhabditis elegans] sp|P34335|KPBA_CAEEL Probable phosphorylase b kinase alpha regulatory chain (Phosphorylase kinase alpha subunit) 0.0
blast CAE70202 Hypothetical protein CBG16677 [Caenorhabditis briggsae] 0.0
blast XP_623102 PREDICTED: similar to ENSANGP00000001572 [Apis mellifera] 0.0
blast EAA06902 ENSANGP00000001572 [Anopheles gambiae str. PEST] ref|XP_311241.2| ENSANGP00000001572 [Anopheles gambiae str. PEST] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:190772_S_AT
gaaagtcacttcggaaatctactcgaagtgtcggaggattggagataccaaaggaggaag
atgaagatgaagaagctgatgaagatgatttccaatttggaatttggctccgtcatcgac
gtattgatggtgcattgaatagagttccaaatggattctatgcgatactctgggatactg
tacataaaatgccacatggagtgaaaatcaatgacactgttttgcattggggattaacac
aagaaatgacccgaaaagaaattaaattcgcattggaatctgaagaagctctaaatagga
ttgcagagcccgaatatcgagagcttgttgtagagactttgtggttattgggaagactgg
aaaagttggtacttcttgagcaaccaaatattccaagagatcgaccattggatgttgatc
acattttgcacgttgcgaatcaaatttttgtagatcataataaacaccttgaaacaattg
taatggaatgttgtgcatcatcaaacccaaataatacgagatgcgatggagctcgcaata
tctgcaagcatttctatgattcggctccagctggagagtatggcaccagtcactacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAGTCACTTCGGAAATCTACTCG 599 359 3158 Antisense
GAATTTGGCTCCGTCATCGACGTAT 431 325 3257 Antisense
GATTCTATGCGATACTCTGGGATAC 528 431 3311 Antisense
TGACACTGTTTTGCATTGGGGATTA 204 569 3369 Antisense
AGGATTGCAGAGCCCGAATATCGAG 415 57 3454 Antisense
GAGAGCTTGTTGTAGAGACTTTGTG 263 395 3476 Antisense
GTTGATCACATTTTGCACGTTGCGA 1 439 3571 Antisense
GGAATGTTGTGCATCATCAAACCCA 232 533 3642 Antisense
GGAGCTCGCAATATCTGCAAGCATT 645 521 3685 Antisense
AGCATTTCTATGATTCGGCTCCAGC 153 79 3704 Antisense
GAGAGTATGGCACCAGTCACTACAT 602 395 3731 Antisense
Sequence Source Affymetrix Proprietary Database