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[190767_s_at] glycosylasparaginase

Description R04B3.2 /REP_DB=WormBase Gene ID /WP=CE07412 /TR=SW:Q21697 /GB=AAA91260.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=glycosylasparaginase
Cluster 6grp_high1_10_10 > 72      6grp_total > 71      4grp_total > 61      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 153
1 2 3 4 range
A 149.2
(3)
A
142.2
(2.3)
A
116.2
(-0.2)
A
88
(-3)
A
61
B 151.6
(3)
A
133
(2.2)
A
122.5
(1.7)
A
19.7
(-3)
A
132
C 151.3
(0.7)
A
117.1
(-3)
A
149.4
(0.5)
A
172.4
(3)
A
55
avr 150.7
(3)
130.8
(0.9)
129.4
(0.8)
93.4
(-3)
57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 311
1 2 3 4 5 6 range
A 113.2
(-3)
A
204.4
(-0.3)
A
309.8
(2.8)
P
316.6
(3)
P
205.2
(-0.3)
P
158.7
(-1.7)
A
203
B 172.8
(-3)
P
214.2
(-1.3)
P
321.1
(3)
P
311.2
(2.6)
P
264.6
(0.7)
P
207.5
(-1.6)
P
148
C 134.1
(-3)
A
211.3
(-1.4)
P
256.1
(-0.5)
P
424.4
(3)
P
226.8
(-1.1)
P
244.9
(-0.7)
P
290
avr 140
(-3)
210
(-1)
295.7
(1.4)
350.7
(3)
232.2
(-0.4)
203.7
(-1.2)
211
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 54.3
(3)
A
30.9
(-0.4)
A
30.4
(-0.5)
A
12.9
(-3)
A
41
B 48.4
(3)
A
32.9
(0.9)
A
28.4
(0.3)
A
4.2
(-3)
A
44
C 50.1
(2.5)
A
33.7
(-1.8)
A
52.1
(3)
A
29.1
(-3)
A
23
avr 50.9
(3)
32.5
(-0.1)
37
(0.7)
15.4
(-3)
36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 20.9
(-3)
A
72.7
(2.2)
A
67.5
(1.7)
P
80.3
(3)
P
61.9
(1.1)
P
38.6
(-1.2)
A
59
B 60.1
(-3)
P
71.3
(-1.6)
P
78
(-0.7)
P
82.4
(-0.2)
P
107.5
(3)
P
77.4
(-0.8)
P
47
C 49.7
(-3)
A
64.5
(-0.5)
P
65.2
(-0.4)
P
85.3
(3)
P
73.4
(1)
P
82
(2.4)
P
36
avr 43.6
(-3)
69.5
(1)
70.2
(1.1)
82.7
(3)
80.9
(2.7)
66
(0.4)
39
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190767_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18570
Sequence Type Exemplar sequence
Representative Public ID R04B3.2 NCBI
Target Description R04B3.2 /REP_DB=WormBase Gene ID /WP=CE07412 /TR=SW:Q21697 /GB=AAA91260.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=glycosylasparaginase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076047(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_076047 NCBI Caenorhabditis elegans R04B3.2 (glycosylasparaginase) mRNA, complete cds. 11/11 None
GENEFINDER00000030424 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2467344:2473577:-1 9/11 None
R04B3.2 ENSEMBL cdna:known chromosome:CEL140:X:2471586:2473667:-1 gene:R04B3.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000030419 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2471585-2473577(-) 100.0 100.0
Public Domain and Genome References
Gene Title glycosylasparaginase
Ensembl R04B3.2
Entrez Gene 180550 Entrez gene
SwissProt Q21697 EMBL-EBI
WormBase CE28745 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247530_AT L-asparaginase, putative / L-asparagine amidohydrolase, putative at
CANINE_2:CFAAFFX.13303.1.S1_S_AT similar to N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase precursor (Glycosylasparaginase) (Aspartylglucosaminidase) (N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase) (AGA) cfa
DROSOPHILA_2:1637955_A_AT dm
DROSGENOME1:152972_AT dm
CHICKEN:GGA.17609.1.S1_S_AT aspartylglucosaminidase gga
CHICKEN:GGA.8163.1.S1_S_AT aspartylglucosaminidase gga
CHICKEN:GGAAFFX.12577.1.S1_AT aspartylglucosaminidase gga
HG-U95E:73299_AT aspartylglucosaminidase hs
HG-U133_PLUS_2:204333_S_AT aspartylglucosaminidase hs
HG-FOCUS:204333_S_AT aspartylglucosaminidase hs
HG-U133A_2:204333_S_AT aspartylglucosaminidase hs
HG-U133A:204333_S_AT aspartylglucosaminidase hs
HUGENEFL:X55330_AT aspartylglucosaminidase hs
U133_X3P:G4557272_3P_A_AT aspartylglucosaminidase hs
U133_X3P:HS.207776.1.S1_3P_A_AT aspartylglucosaminidase hs
U133_X3P:HS.207776.0.S1_3P_A_AT aspartylglucosaminidase hs
HG-U133A_2:204332_S_AT aspartylglucosaminidase hs
HG-U133_PLUS_2:204332_S_AT aspartylglucosaminidase hs
HG-U133A:204332_S_AT aspartylglucosaminidase hs
HG-U133A_2:216064_S_AT aspartylglucosaminidase hs
HG-U133_PLUS_2:216064_S_AT aspartylglucosaminidase hs
HG-U133A:216064_S_AT aspartylglucosaminidase hs
HG-U95AV2:34181_AT aspartylglucosaminidase hs
HG-U95E:80461_AT aspartylglucosaminidase hs
HU35KSUBA:W27131_AT Aspartylglucosaminidase hs
MG-U74BV2:163631_AT aspartylglucosaminidase mm
MOE430B:1434665_AT aspartylglucosaminidase mm
MOUSE430_2:1434665_AT aspartylglucosaminidase mm
MU19KSUBC:TC41556_AT Aspartylglucosaminidase (Aga), mRNA mm
RG-U34B:RC_AA943766_AT aspartylglucosaminidase (predicted) rn
RAE230A:1372421_AT aspartylglucosaminidase (predicted) rn
RAT230_2:1372421_AT aspartylglucosaminidase (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6516 glycoprotein catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4067 asparaginase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA91260 Hypothetical protein R04B3.2 [Caenorhabditis elegans] ref|NP_508448.1| R04B3.2 [Caenorhabditis elegans] sp|Q21697|ASPG_CAEEL Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase precursor (Glycosylasparaginase) (Aspartylglucosaminidase) (N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase) (AGA) [Contains: Glycosylasparaginase alpha chain; Glycosylasparaginase beta chain] 0.0
blast T30965 hypothetical protein R04B3.2 - Caenorhabditis elegans 0.0
blast AAA91259 Hypothetical protein R04B3.1 [Caenorhabditis elegans] ref|NP_508447.2| R04B3.1 [Caenorhabditis elegans] 0.0
blast CAE58874 Hypothetical protein CBG02105 [Caenorhabditis briggsae] 0.0
blast CAE58873 Hypothetical protein CBG02104 [Caenorhabditis briggsae] 1.0E-150
Protein Domains
Database ID Description E-Value
Pfam IPR000246 EMBL-EBI Peptidase T2, asparaginase 2 1.0E-126
Pfam IPR000246 EMBL-EBI Peptidase T2, asparaginase 2 1.0E-126
Sequence
Target Sequence
>C. ELEGANS:190767_S_AT
gtctgacgaagctggatatctggttgaaaaaacaaatcatgacacaattggaatggtggt
acgcgacacggaaaacatattcagcgccggaacatcatcaaatggagctagattcaaaat
tcccggtcgagttggtgatagtccgattccgggtgctggagcatatgcaaacaagttcgg
tggagccgccgcaactggtgacggtgatgtcatgatgaggtttttaccaagcttctttgc
tgtaacacagatggaactgggtacaaagccatcaaaagcagcttataaggcgataactcg
aattctgaaagttttcccgaaattttcaggcgcagtcgttgcaatgaatgtgaaaggcag
aatcggagcctcgtgtgcgaatattaataaattcggatacaatgtggcttttcaaaacgg
aacggtggtgacctactccataagt


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GTCTGACGAAGCTGGATATCTGGTT 481 471 648 Antisense
GGAATGGTGGTACGCGACACGGAAA 258 533 697 Antisense
ACATATTCAGCGCCGGAACATCATC 343 105 722 Antisense
AGTTGGTGATAGTCCGATTCCGGGT 679 63 777 Antisense
GATTCCGGGTGCTGGAGCATATGCA 314 433 792 Antisense
CAAACAAGTTCGGTGGAGCCGCCGC 496 191 815 Antisense
CCGCCGCAACTGGTGACGGTGATGT 650 259 833 Antisense
GAGGTTTTTACCAAGCTTCTTTGCT 663 399 864 Antisense
GAAATTTTCAGGCGCAGTCGTTGCA 489 351 966 Antisense
TCGGAGCCTCGTGTGCGAATATTAA 166 605 1010 Antisense
AACGGTGGTGACCTACTCCATAAGT 306 143 1068 Antisense
Sequence Source Affymetrix Proprietary Database