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[190766_s_at] zinc metalloprotease

Description T04G9.2 /REP_DB=WormBase Gene ID /WP=CE04883 /TR=SW:P55115 /GB=AAA82461.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster 6grp_total > 82      4grp_total > 49      6grp_cgc > 64      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1241
1 2 3 4 range
A 49
(-3)
A 		1175.8
(3)
P 		1107.4
(2.6)
P 		92.3
(-2.8)
A 		1127
B 24.2
(-2.9)
A 		1012.1
(1.8)
P 		1250
(3)
P 		12
(-3)
A 		1238
C 9.5
(-3)
A 		1153.5
(3)
P 		1091.9
(2.7)
P 		45.5
(-2.8)
A 		1144
avr 27.6
(-3) 		1113.8
(2.8) 		1149.8
(3) 		49.9
(-2.9) 		1122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 882
1 2 3 4 5 6 range
A 81.3
(-3)
A 		917.5
(2.9)
P 		463.5
(-0.3)
P 		679.3
(1.2)
P 		926.9
(3)
P 		325.7
(-1.3)
P 		846
B 145.7
(-3)
M 		759.7
(2.3)
P 		510.3
(0.2)
P 		838.1
(3)
P 		835.1
(3)
P 		311.8
(-1.6)
P 		692
C 58.4
(-3)
A 		769.1
(1.8)
P 		541.1
(0.3)
P 		626.8
(0.9)
P 		940.8
(3)
P 		273.4
(-1.5)
P 		882
avr 95.1
(-3) 		815.4
(2.4) 		505
(0.1) 		714.7
(1.6) 		900.9
(3) 		303.6
(-1.4) 		806
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 378
1 2 3 4 range
A 17.8
(-2.9)
A 		255.2
(2.3)
P 		289.5
(3)
P 		13.5
(-3)
A 		276
B 7.7
(-2.9)
A 		250
(2.2)
P 		289.7
(3)
P 		2.6
(-3)
A 		287
C 3.2
(-3)
A 		331.6
(2.2)
P 		380.5
(3)
P 		7.7
(-2.9)
A 		377
avr 9.6
(-3) 		278.9
(2.2) 		319.9
(3) 		7.9
(-3) 		312

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 324
1 2 3 4 5 6 range
A 15
(-3)
A 		326.3
(3)
P 		101
(-1.3)
P 		172.3
(0)
P 		279.7
(2.1)
P 		79.3
(-1.8)
P 		311
B 50.7
(-3)
M 		252.9
(1.2)
P 		123.9
(-1.5)
P 		222
(0.6)
P 		339.3
(3)
P 		116.3
(-1.6)
P 		289
C 21.7
(-3)
A 		234.7
(1.5)
P 		137.7
(-0.5)
P 		126.1
(-0.8)
P 		304.4
(3)
P 		91.5
(-1.5)
P 		283
avr 29.1
(-3) 		271.3
(2.2) 		120.9
(-1) 		173.5
(0.1) 		307.8
(3) 		95.7
(-1.6) 		279
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190766_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18457
Sequence Type Exemplar sequence
Representative Public ID T04G9.2 NCBI
Target Description T04G9.2 /REP_DB=WormBase Gene ID /WP=CE04883 /TR=SW:P55115 /GB=AAA82461.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075753(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075753 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-15) (nas-15) mRNA, complete cds. 11/11 None
SNAP00000034734 ENSEMBL cdna:SNAP chromosome:CEL140:X:777733:780234:1 9/11 None
GENEFINDER00000034738 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:777733:780234:1 11/11 None
T04G9.2 ENSEMBL cdna:known chromosome:CEL140:X:776196:780472:1 gene:T04G9.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:776195-780234(+) 100.0 100.0
Public Domain and Genome References
Gene Title zinc metalloprotease
Gene Symbol nas-15
Ensembl T04G9.2
Entrez Gene 180426 Entrez gene
WormBase CE38541 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_508154 Nematode AStacin protease family member (nas-15) [Caenorhabditis elegans] gb|AAA82461.2| Nematode astacin protease protein 15 [Caenorhabditis elegans] sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precursor (Nematode astacin 15) 0.0
blast CAE63622 Hypothetical protein CBG08116 [Caenorhabditis briggsae] 0.0
blast CAD99210 NAS-15 protein [Caenorhabditis elegans] 1.0E-146
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.1E-118
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.6E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.3E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.9E-13
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.1E-118
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.6E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.3E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.9E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.9E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
T04G9.2 1 54-71
Sequence
Target Sequence 
>C. ELEGANS:190766_S_AT
gtgccaactgcccaatttcatgtggaatgtgcattccaaccaaggaaactcagaagccat
atgtccaaactacgacccaagctgcgactacaaccgccagaccacaaaagccagtcaccc
aaccaattcaaccccttccaccagtcccgccgcttcctccaactaccccagaagattgtg
aggatcttcgtgtcgattgtcttgtattggtttctcagagatactgcaaaatttcacaaa
acttcatgaaatcttattgtgcaaagagctgcggcttctgcttcaagccacctccaaccg
agattccggataaccgcccgactgtggtaaccactagaccactagtcacacttccaccag
cggtcatccggtcgcgttctccggctccgcccgtttccaccacgaccaaggctgctccaa
caaccagtacaactagcgcagctccttactccccaactccactacccagcgagtgctccg
atcgcaaacatttttgctcacactggaagagcgctggattctgtgaagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCCAACTGCCCAATTTCATGTGG 75 479 1295 Antisense
GCCATATGTCCAAACTACGACCCAA 219 275 1350 Antisense
GAGGATCTTCGTGTCGATTGTCTTG 353 403 1474 Antisense
CGATTGTCTTGTATTGGTTTCTCAG 150 249 1488 Antisense
TGTGCAAAGAGCTGCGGCTTCTGCT 204 557 1552 Antisense
CCACCTCCAACCGAGATTCCGGATA 592 271 1582 Antisense
CCGCCCGACTGTGGTAACCACTAGA 66 263 1608 Antisense
ACCACTAGACCACTAGTCACACTTC 404 85 1624 Antisense
ACAACCAGTACAACTAGCGCAGCTC 517 113 1714 Antisense
CGAGTGCTCCGATCGCAAACATTTT 97 249 1764 Antisense
GGAAGAGCGCTGGATTCTGTGAAGG 569 531 1799 Antisense
Sequence Source Affymetrix Proprietary Database