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[190740_at] 6-phosphofructokinase

Description C50F4.2 /REP_DB=WormBase Gene ID /WP=CE05467 /TR=SW:Q27483 /GB=CAA94737.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=6-phosphofructokinase
Cluster 6grp_total > 89      4grp_total > 20      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 37.3
(-2.7)
A 		48.5
(-1.9)
A 		33.1
(-3)
A 		116.2
(3)
A 		83
B 54.7
(-0.7)
A 		74.4
(3)
A 		58.4
(-0)
A 		42.4
(-3)
A 		32
C 66.9
(0.4)
A 		51.1
(-0.5)
A 		6.9
(-3)
A 		111.3
(3)
A 		104
avr 53
(-0.9) 		58
(-0.4) 		32.8
(-3) 		90
(3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 89.2
(0.8)
A 		30.9
(-3)
A 		114.3
(2.4)
A 		30.2
(-3)
A 		115.1
(2.4)
A 		124
(3)
A 		94
B 75.6
(0.2)
A 		24.1
(-2.2)
A 		36.1
(-1.6)
A 		5.4
(-3)
A 		117.7
(2.1)
M 		138.1
(3)
P 		133
C 21.6
(-2.2)
A 		72.4
(0.7)
A 		7.4
(-3)
A 		10.4
(-2.8)
A 		65.5
(0.3)
A 		112
(3)
P 		105
avr 62.1
(-0.4) 		42.5
(-1.5) 		52.6
(-1) 		15.3
(-3) 		99.4
(1.6) 		124.7
(3) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 13.6
(0.5)
A 		10.5
(-1.7)
A 		8.7
(-3)
A 		17
(3)
A 		8
B 17.4
(2.4)
A 		18.4
(3)
A 		13.5
(-0.1)
A 		9
(-3)
A 		9
C 22.1
(3)
A 		14.7
(0.7)
A 		2.4
(-3)
A 		18.8
(2)
A 		20
avr 17.7
(3) 		14.5
(1) 		8.2
(-3) 		14.9
(1.2) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 16.4
(-1.1)
A 		11
(-2.3)
A 		24.9
(0.8)
A 		7.7
(-3)
A 		34.7
(3)
A 		30.2
(2)
A 		27
B 26.3
(-0)
A 		8
(-2.2)
A 		8.8
(-2.1)
A 		1.4
(-3)
A 		47.8
(2.6)
M 		51.5
(3)
P 		50
C 8
(-2)
A 		22.1
(0.4)
A 		1.9
(-3)
A 		2.1
(-3)
A 		21.2
(0.3)
A 		37.5
(3)
P 		36
avr 16.9
(-0.8) 		13.7
(-1.3) 		11.9
(-1.6) 		3.7
(-3) 		34.6
(2.2) 		39.7
(3) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190740_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11773
Sequence Type Exemplar sequence
Representative Public ID C50F4.2 NCBI
Target Description C50F4.2 /REP_DB=WormBase Gene ID /WP=CE05467 /TR=SW:Q27483 /GB=CAA94737.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=6-phosphofructokinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073056(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073056 NCBI Caenorhabditis elegans C50F4.2 (6-phosphofructokinase) mRNA, complete cds. 11/11 None
SNAP00000009310 ENSEMBL cdna:SNAP chromosome:CEL140:V:9534415:9536951:1 11/11 None
GENEFINDER00000009329 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9534415:9538942:1 11/11 None
C50F4.2 ENSEMBL cdna:known chromosome:CEL140:V:9534403:9537121:1 gene:C50F4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9534414-9537010(+) 100.0 100.0
Public Domain and Genome References
Gene Title 6-phosphofructokinase
Ensembl C50F4.2
Entrez Gene 179335 Entrez gene
SwissProt Q27483 EMBL-EBI
WormBase CE05467 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6096 glycolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5945 6-phosphofructokinase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3872 6-phosphofructokinase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94737 Hypothetical protein C50F4.2 [Caenorhabditis elegans] ref|NP_505457.1| C50F4.2 [Caenorhabditis elegans] sp|Q27483|K6PF_CAEEL Probable 6-phosphofructokinase (Phosphofructokinase) (Phosphohexokinase) 0.0
blast CAE72040 Hypothetical protein CBG19122 [Caenorhabditis briggsae] 0.0
blast CAA94738 Hypothetical protein C50F4.3 [Caenorhabditis elegans] ref|NP_505458.1| Temporarily Assigned Gene name family member (tag-329) [Caenorhabditis elegans] 0.0
blast XP_782075 PREDICTED: similar to 6-phosphofructokinase, muscle type (Phosphofructokinase 1) (Phosphohexokinase) (Phosphofructo-1-kinase isozyme A) (PFK-A) (Phosphofructokinase-M) [Strongylocentrotus purpuratus] 1.0E-161
Protein Domains
Database ID Description E-Value
Pfam IPR000023 EMBL-EBI Phosphofructokinase 1.0E-126
Pfam IPR000023 EMBL-EBI Phosphofructokinase 6.2E-33
Pfam IPR000023 EMBL-EBI Phosphofructokinase 3.5E-12
Pfam IPR000023 EMBL-EBI Phosphofructokinase 5.4E-17
Pfam IPR000668 EMBL-EBI Peptidase C1A, papain 8.9E-64
Sequence
Target Sequence 
>C. ELEGANS:190740_AT
aatgggatcacgctctggatttcttgccactatgacagctctttctactggatcccagtt
tgcacttatcagacaagttgaaacaaatgagaaagatctagaaaagttggcgattgaaac
taaagagcggctggattcagggaaccttgagaagttcttgttgattcgttcagaaggtgc
aagtgacgaaatatattctccggatgttaagaagatatttgacaaagtgatgaagaataa
gtatggagttcgtattactaaccttggatatagtcaactcggtggacatccgtcctgttt
cgatcgccaaatgggaattcgaatgggagttcgtgcgttcgaaggaatcgtgaaccctgt
aaaaatgggagatagggattgctgtgtcattggattgagaggaagcagtctgagatatgt
tcctgttcaaggactcggcaagaaagtgtgcttcgagcatggagttccacataacatgtg
gtggttggatttgcatccccttgttgaagcaatgacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGGGATCACGCTCTGGATTTCTT 470 163 1734 Antisense
GGATTTCTTGCCACTATGACAGCTC 66 511 1750 Antisense
TATGACAGCTCTTTCTACTGGATCC 54 657 1764 Antisense
GGATCCCAGTTTGCACTTATCAGAC 459 513 1783 Antisense
GAGCGGCTGGATTCAGGGAACCTTG 125 385 1858 Antisense
TCAACTCGGTGGACATCCGTCCTGT 355 631 2007 Antisense
TCCTGTTTCGATCGCCAAATGGGAA 278 593 2026 Antisense
GCAGTCTGAGATATGTTCCTGTTCA 459 311 2138 Antisense
GTGCTTCGAGCATGGAGTTCCACAT 562 479 2181 Antisense
TGTGGTGGTTGGATTTGCATCCCCT 375 555 2210 Antisense
GCATCCCCTTGTTGAAGCAATGACC 566 307 2226 Antisense
Sequence Source Affymetrix Proprietary Database