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[190734_at] aspartyl-tRNA synthetase

Description F10C2.6 /REP_DB=WormBase Gene ID /WP=CE20663 /TR=P90831 /GB=CAB04081.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=aspartyl-tRNA synthetase
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 43      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 52      6grp_total > 20      4grp_total > 61      6grp_highest_sub > 02_03      6grp_cgc > 72      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 360
1 2 3 4 range
A 659.8
(0.8)
P 		646.8
(0.6)
P 		784.6
(3)
P 		444.8
(-3)
P 		340
B 758.4
(3)
P 		718.6
(2.3)
P 		719.4
(2.3)
P 		424.4
(-3)
P 		334
C 667.7
(3)
P 		553.2
(-0.5)
P 		641.9
(2.2)
P 		473.2
(-3)
P 		195
avr 695.3
(2.6) 		639.5
(1.3) 		715.3
(3) 		447.5
(-3) 		268

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 539
1 2 3 4 5 6 range
A 484.8
(-1.5)
P 		675
(0.8)
P 		643.3
(0.4)
P 		861.4
(3)
P 		361.8
(-3)
P 		575.8
(-0.4)
P 		500
B 540.5
(-2.5)
P 		898.7
(3)
P 		834.6
(2)
P 		506.2
(-3)
P 		587.1
(-1.8)
P 		568.5
(-2)
P 		393
C 509.4
(-1.9)
P 		878.2
(2.7)
P 		901.1
(3)
P 		508.7
(-2)
P 		520.6
(-1.8)
P 		426.3
(-3)
P 		475
avr 511.6
(-2.6) 		817.3
(3) 		793
(2.6) 		625.4
(-0.5) 		489.8
(-3) 		523.5
(-2.4) 		328
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 240.2
(3)
P 		140.4
(-0.4)
P 		205.1
(1.8)
P 		65.1
(-3)
P 		175
B 241.8
(3)
P 		177.5
(0.5)
P 		166.7
(0)
P 		90.4
(-3)
P 		151
C 221
(2.9)
P 		159
(0.3)
P 		223.7
(3)
P 		79.8
(-3)
P 		144
avr 234.3
(3) 		159
(0.1) 		198.5
(1.6) 		78.4
(-3) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 89.4
(-3)
P 		240.1
(3)
P 		140.1
(-1)
P 		218.5
(2.1)
P 		109.2
(-2.2)
P 		140.2
(-1)
P 		151
B 188.1
(-1)
P 		299.2
(3)
P 		202.7
(-0.5)
P 		134.1
(-3)
P 		238.5
(0.8)
P 		212
(-0.2)
P 		165
C 188.8
(0.1)
P 		268
(3)
P 		229.3
(1.6)
P 		102.3
(-3)
P 		168.5
(-0.6)
P 		142.7
(-1.5)
P 		166
avr 155.4
(-2.8) 		269.1
(3) 		190.7
(-1) 		151.6
(-3) 		172.1
(-2) 		165
(-2.3) 		118
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190734_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13615
Sequence Type Exemplar sequence
Representative Public ID F10C2.6 NCBI
Target Description F10C2.6 /REP_DB=WormBase Gene ID /WP=CE20663 /TR=P90831 /GB=CAB04081.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=aspartyl-tRNA synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073618(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073618 NCBI Caenorhabditis elegans aspartyl(D) tRNA Synthetase family member (drs-2) (drs-2) mRNA, complete cds. 11/11 None
SNAP00000025561 ENSEMBL cdna:SNAP chromosome:CEL140:V:12051334:12054659:1 11/11 None
GENEFINDER00000025566 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12051334:12054669:1 11/11 None
F10C2.6 ENSEMBL cdna:known chromosome:CEL140:V:12051333:12054733:1 gene:F10C2.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12051527-12054659(+) 100.0 100.0
Public Domain and Genome References
Gene Title aspartyl-tRNA synthetase
Gene Symbol drs-2
Ensembl F10C2.6
Entrez Gene 3565124 Entrez gene
SwissProt P90831 EMBL-EBI
WormBase CE31906 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:253295_AT tRNA synthetase class II (D, K and N) family protein at
CANINE_2:CFAAFFX.22393.1.S1_AT similar to aspartyl-tRNA synthetase 2 (mitochondrial) cfa
DROSOPHILA_2:1629374_AT dm
DROSGENOME1:146383_AT dm
CHICKEN:GGAAFFX.2261.2.S1_S_AT similar to hypothetical protein FLJ10514 gga
CHICKEN:GGAAFFX.2261.5.S1_S_AT similar to hypothetical protein FLJ10514 gga
HU35KSUBC:RC_AA287138_AT aspartyl-tRNA synthetase 2 (mitochondrial) hs
U133_X3P:HS.59346.0.S1_3P_A_AT aspartyl-tRNA synthetase 2 (mitochondrial) hs
HG-U133_PLUS_2:218365_S_AT aspartyl-tRNA synthetase 2 (mitochondrial) hs
HG-U133A:218365_S_AT aspartyl-tRNA synthetase 2 (mitochondrial) hs
HG-U133A_2:218365_S_AT aspartyl-tRNA synthetase 2 (mitochondrial) hs
HG-U95C:64470_AT aspartyl-tRNA synthetase 2 (mitochondrial) hs
HG-U95E:86801_AT aspartyl-tRNA synthetase 2 (mitochondrial) hs
HG-U95E:86803_AT Aspartyl-tRNA synthetase 2 (mitochondrial) hs
MG-U74BV2:108064_AT aspartyl-tRNA synthetase 2 (mitochondrial) mm
MOE430B:1433838_AT aspartyl-tRNA synthetase 2 (mitochondrial) mm
MOUSE430_2:1433838_AT aspartyl-tRNA synthetase 2 (mitochondrial) mm
MU19KSUBB:TC32059_AT Aspartyl-tRNA synthetase 2 (mitochondrial), mRNA (cDNA clone MGC:99963 IMAGE:30473852) mm
RG-U34B:RC_AA901017_AT Similar to RIKEN cDNA 5830468K18 (predicted) rn
RG-U34C:RC_AI172076_AT Similar to RIKEN cDNA 5830468K18 (predicted) rn
RAE230A:1376135_AT Similar to RIKEN cDNA 5830468K18 (predicted) rn
RAT230_2:1376135_AT Similar to RIKEN cDNA 5830468K18 (predicted) rn
RAE230B:1398475_AT Similar to RIKEN cDNA 5830468K18 (predicted) rn
RAT230_2:1398475_AT Similar to RIKEN cDNA 5830468K18 (predicted) rn
YEAST_2:1776799_AT Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
6422 aspartyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
6430 lysyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
4815 aspartate-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
4824 lysine-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04081 Hypothetical protein F10C2.6 [Caenorhabditis elegans] ref|NP_506019.2| aspartyl(D) tRNA Synthetase family member (drs-2) [Caenorhabditis elegans] 0.0
blast CAE75375 Hypothetical protein CBG23362 [Caenorhabditis briggsae] 0.0
blast EAA12212 ENSANGP00000018448 [Anopheles gambiae str. PEST] ref|XP_317655.2| ENSANGP00000018448 [Anopheles gambiae str. PEST] 1.0E-119
blast EAA12212 ENSANGP00000018448 [Anopheles gambiae str. PEST] ref|XP_317655.2| ENSANGP00000018448 [Anopheles gambiae str. PEST] 1.0E-118
Protein Domains
Database ID Description E-Value
Pfam IPR004365 EMBL-EBI OB-fold nucleic acid binding domain 3.0E-13
Pfam IPR004364 EMBL-EBI tRNA synthetase, class II (D, K and N) 3.0E-18
Pfam IPR004364 EMBL-EBI tRNA synthetase, class II (D, K and N) 1.8E-14
Pfam IPR004364 EMBL-EBI tRNA synthetase, class II (D, K and N) 1.2E-10
Pfam IPR004364 EMBL-EBI tRNA synthetase, class II (D, K and N) 1.8E-14
Pfam IPR004364 EMBL-EBI tRNA synthetase, class II (D, K and N) 1.8E-14
Sequence
Target Sequence 
>C. ELEGANS:190734_AT
gtaactgctcactggattgtggattttccattgttctcatttgaagaaggacaacttgta
tcaacacatcatccatttacggctccacttgaaaaagatattgatattctttattccaat
gatattgataaactcttacaaatcactggacaacactacgatcttgttataaacggagtt
gaaatgggtggtggatcgattagaattgagaattcagaaatccagcgacacgttctgaaa
gttcttggagaaccaactgatgaaatggaacatcttttaaatgcattatctcatggtgca
ccaccacatggaggatttgctttaggacttgatagattcgttgcaatgcttacttccgat
gggaatcctctcacaccagttcgagatgttattgcttttccaaaaacaaaaaatggaaaa
gatttgatgagtgatgcgcctgcaacattatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAACTGCTCACTGGATTGTGGATT 2 463 1216 Antisense
CAACACATCATCCATTTACGGCTCC 670 189 1277 Antisense
AATCACTGGACAACACTACGATCTT 359 171 1356 Antisense
GAATTCAGAAATCCAGCGACACGTT 41 323 1425 Antisense
GCGACACGTTCTGAAAGTTCTTGGA 173 289 1440 Antisense
TGCATTATCTCATGGTGCACCACCA 564 579 1497 Antisense
GTGCACCACCACATGGAGGATTTGC 360 481 1511 Antisense
GATTCGTTGCAATGCTTACTTCCGA 530 431 1550 Antisense
ACTTCCGATGGGAATCCTCTCACAC 491 103 1567 Antisense
AATCCTCTCACACCAGTTCGAGATG 645 167 1579 Antisense
GAGTGATGCGCCTGCAACATTATCC 397 401 1644 Antisense
Sequence Source Affymetrix Proprietary Database