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[190726_at] nucleoside transporter

Description F27E11.1 /REP_DB=WormBase Gene ID /WP=CE09732 /TR=O16192 /GB=AAB65255.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=nucleoside transporter
Cluster 6grp_high1_10_10 > 52      4grp_high_8_8 > 28      6grp_total > 82      4grp_total > 21      6grp_high1_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 100.2
(2.2)
P 		77.1
(-3)
M 		97.2
(1.5)
P 		103.9
(3)
P 		27
B 81.4
(-3)
P 		90.9
(-0.6)
A 		94.8
(0.3)
P 		105.6
(3)
A 		24
C 121.7
(2.8)
P 		119.9
(2.6)
P 		70.4
(-3)
P 		123.9
(3)
P 		54
avr 101.1
(0.5) 		96
(-0.8) 		87.5
(-3) 		111.1
(3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 87.5
(-0.1)
P 		70.6
(-2.4)
P 		111.2
(3)
P 		99.8
(1.5)
M 		106.7
(2.4)
P 		65.8
(-3)
A 		45
B 67.7
(-2.9)
P 		123.3
(3)
P 		69
(-2.7)
M 		108.5
(1.4)
A 		109.9
(1.6)
P 		66.4
(-3)
P 		57
C 58.1
(-3)
P 		99.8
(2.7)
P 		92.3
(1.7)
M 		101.9
(3)
P 		78
(-0.3)
P 		58.2
(-3)
A 		44
avr 71.1
(-1.9) 		97.9
(2.2) 		90.8
(1.1) 		103.4
(3) 		98.2
(2.2) 		63.5
(-3) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 36.5
(3)
P 		16.7
(-2.6)
M 		25.4
(-0.1)
P 		15.2
(-3)
P 		21
B 25.9
(3)
P 		22.5
(-2.2)
A 		22
(-3)
P 		22.5
(-2.2)
A 		4
C 40.3
(3)
P 		34.5
(1.2)
P 		24.5
(-1.9)
P 		20.9
(-3)
P 		19
avr 34.2
(3) 		24.6
(-0.9) 		24
(-1.2) 		19.5
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 16.1
(-3)
P 		25.1
(0.4)
P 		24.2
(0)
P 		25.3
(0.4)
M 		32.2
(3)
P 		16
(-3)
A 		16
B 23.5
(-1.6)
P 		41
(2.2)
P 		16.8
(-3)
M 		28.7
(-0.4)
A 		44.6
(3)
P 		24.8
(-1.3)
P 		28
C 21.5
(-1.9)
P 		30.4
(3)
P 		23.5
(-0.8)
M 		20.5
(-2.4)
P 		25.2
(0.1)
P 		19.5
(-3)
A 		11
avr 20.4
(-2.9) 		32.2
(2.2) 		21.5
(-2.4) 		24.8
(-1) 		34
(3) 		20.1
(-3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190726_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15679
Sequence Type Exemplar sequence
Representative Public ID F27E11.1 NCBI
Target Description F27E11.1 /REP_DB=WormBase Gene ID /WP=CE09732 /TR=O16192 /GB=AAB65255.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=nucleoside transporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071566(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071566 NCBI Caenorhabditis elegans F27E11.1 (F27E11.1) mRNA, complete cds. 11/11 None
SNAP00000039155 ENSEMBL cdna:SNAP chromosome:CEL140:V:3459565:3465965:1 9/11 None
GENEFINDER00000039158 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3463561:3465943:1 11/11 None
F27E11.1 ENSEMBL cdna:known chromosome:CEL140:V:3463640:3465976:1 gene:F27E11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3463659-3465965(+) 100.0 100.0
Public Domain and Genome References
Gene Title nucleoside transporter
Ensembl F27E11.1
Entrez Gene 178770 Entrez gene
SwissProt O16192 EMBL-EBI
WormBase CE09732 Wormbase
Functional Annotations
Ortholog
ID Title Organism
HG-U133_PLUS_2:207249_S_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 hs
HG-U133A:207249_S_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 hs
HG-FOCUS:207249_S_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 hs
HG-U133A_2:207249_S_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 hs
U133_X3P:G4759131_3P_S_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 hs
U133_X3P:207249_3P_S_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 hs
HG-U95AV2:34057_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 hs
HG-U133_PLUS_2:216432_AT Solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 hs
HG-U133A_2:216432_AT Solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 hs
HG-U133A:216432_AT Solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 hs
MOE430A:1450639_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 mm
MOUSE430A_2:1450639_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 mm
MOUSE430_2:1450639_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 mm
MU19KSUBC:TC37682_AT Purine-selective Na+ nucleoside cotransporter (Cnt2) mm
RG-U34A:U66723_S_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 rn
RG-U34B:RC_AA945453_S_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 rn
RAE230A:1368227_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 rn
RAT230_2:1368227_AT solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5415 nucleoside:sodium symporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB65255 Hypothetical protein F27E11.1 [Caenorhabditis elegans] ref|NP_503967.1| F27E11.1 [Caenorhabditis elegans] gb|AAF80461.1| CNT-like nucleoside transporter [Caenorhabditis elegans] 0.0
blast CAE74621 Hypothetical protein CBG22412 [Caenorhabditis briggsae] 0.0
blast CAE74622 Hypothetical protein CBG22413 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002668 EMBL-EBI Na+ dependent nucleoside transporter 1.0E-126
Pfam IPR002668 EMBL-EBI Na+ dependent nucleoside transporter 1.0E-126
Pfam IPR002668 EMBL-EBI Na+ dependent nucleoside transporter 1.0E-126
Pfam IPR002668 EMBL-EBI Na+ dependent nucleoside transporter 4.8E-56
Pfam IPR002668 EMBL-EBI Na+ dependent nucleoside transporter 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503967.1 12 40-62,72-89,110-132,142-159,164-186,196-218,239-258,307-329,386-408,423-445,489-511,526-548
F27E11.1 12 40-62,72-89,110-132,142-159,164-186,196-218,239-258,307-329,386-408,423-445,489-511,526-548
SNAP00000039155 13 15-34,46-68,83-105,154-176,235-257,270-292,330-352,364-386,401-423,475-497,556-578,625-647,662-684
GENEFINDER00000039158 11 41-63,73-90,111-133,143-160,165-187,197-219,240-259,308-330,387-409,424-446,528-550
Sequence
Target Sequence 
>C. ELEGANS:190726_AT
agaagacttcgagctggaccatgagaacgaaaaaggattcttcgatactctgtgctcggc
gggagttgctctagtccctacggtttttgcaattggagcaactttagtagtagtcatgtc
tctattagctctgctggaccaaattttcttctacattggtgacctaatcgggtatgatgg
gtggagttttcaaatgttatttggttatgcatttttcccgttggcatacataatgggtat
taccaataattcgaatcaaaccttgttagtggctcaacttatgggctccaaaactgccgt
aaacgaatttgtggcatatgataaactgggtcaacttcaaaaagaaggcaagctggatcc
caaatcagtgctagttgccacttacgcactctgtggattctccaattttggctcaatggg
gattcaaatggaagttattggaggtctggctccagggaggaaaaccgtggcttccaaggt
tatgctccgtgctctttgtgcaggagccatcgcttgttttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAGACTTCGAGCTGGACCATGAG 12 73 1107 Antisense
AGGATTCTTCGATACTCTGTGCTCG 375 57 1140 Antisense
AGTCATGTCTCTATTAGCTCTGCTG 465 61 1218 Antisense
GCTGGACCAAATTTTCTTCTACATT 240 295 1239 Antisense
GCATTTTTCCCGTTGGCATACATAA 143 309 1315 Antisense
TAGTGGCTCAACTTATGGGCTCCAA 380 653 1373 Antisense
GGCAAGCTGGATCCCAAATCAGTGC 306 535 1453 Antisense
AGTGCTAGTTGCCACTTACGCACTC 118 61 1473 Antisense
TTACGCACTCTGTGGATTCTCCAAT 533 681 1488 Antisense
ACCGTGGCTTCCAAGGTTATGCTCC 577 87 1570 Antisense
GTGCAGGAGCCATCGCTTGTTTTAT 102 481 1604 Antisense
Sequence Source Affymetrix Proprietary Database