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[190720_at]

Description ZK970.1 /REP_DB=WormBase Gene ID /WP=CE26353 /TR=Q23684 /GB=CAA88893.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=neprilysin zinc protease
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 15      4grp_highest_8_8 > 52      4grp_high_8_8 > 24      6grp_highest_nonorm > 80      6grp_total > 25      4grp_total > 39      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 358
1 2 3 4 range
A 490.2
(3)
P 		194.8
(-2.7)
P 		200.6
(-2.5)
P 		176.8
(-3)
P 		313
B 479.6
(3)
P 		222
(-1.9)
P 		161.5
(-3)
P 		301.5
(-0.4)
P 		318
C 465.7
(3)
P 		137.1
(-2.9)
P 		132.6
(-3)
P 		269.9
(-0.5)
P 		333
avr 478.5
(3) 		184.6
(-2.6) 		164.9
(-3) 		249.4
(-1.4) 		314

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 233
1 2 3 4 5 6 range
A 190.1
(-0.4)
P 		275.3
(3)
P 		164.9
(-1.4)
P 		159.4
(-1.6)
P 		123.4
(-3)
P 		150.6
(-1.9)
P 		152
B 338.2
(3)
P 		254.8
(0.8)
P 		158.4
(-1.8)
P 		159.3
(-1.8)
P 		126.5
(-2.7)
P 		114.6
(-3)
P 		224
C 323.9
(3)
P 		246.5
(0.9)
P 		195.7
(-0.5)
P 		135.6
(-2.2)
P 		104.8
(-3)
P 		124
(-2.5)
P 		219
avr 284.1
(3) 		258.9
(2.1) 		173
(-1) 		151.4
(-1.8) 		118.2
(-3) 		129.7
(-2.6) 		166
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 153
1 2 3 4 range
A 178.5
(3)
P 		42.3
(-2.4)
P 		52.4
(-2)
P 		25.9
(-3)
P 		153
B 152.9
(3)
P 		54.8
(-2.1)
P 		37.4
(-3)
P 		64.3
(-1.6)
P 		116
C 154.2
(3)
P 		39.4
(-3)
P 		46.2
(-2.6)
P 		45.5
(-2.7)
P 		115
avr 161.9
(3) 		45.5
(-3) 		45.3
(-3) 		45.2
(-3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 35.1
(-3)
P 		97.9
(3)
P 		35.9
(-2.9)
P 		40.4
(-2.5)
P 		37.3
(-2.8)
P 		36.7
(-2.8)
P 		63
B 117.7
(3)
P 		84.8
(0.5)
P 		38.5
(-3)
P 		42.2
(-2.7)
P 		51.4
(-2)
P 		42.7
(-2.7)
P 		79
C 120.1
(3)
P 		75.2
(0.1)
P 		49.8
(-1.5)
P 		27.3
(-3)
P 		33.9
(-2.6)
P 		41.5
(-2.1)
P 		93
avr 91
(3) 		86
(2.4) 		41.4
(-2.5) 		36.6
(-3) 		40.9
(-2.5) 		40.3
(-2.6) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190720_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3623
Sequence Type Exemplar sequence
Representative Public ID ZK970.1 NCBI
Target Description ZK970.1 /REP_DB=WormBase Gene ID /WP=CE26353 /TR=Q23684 /GB=CAA88893.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=neprilysin zinc protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027368(9), NM_001027369(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027368 NCBI Caenorhabditis elegans ZK970.1a (ZK970.1) mRNA, complete cds. 9/11 None
NM_001027369 NCBI Caenorhabditis elegans ZK970.1b (ZK970.1) mRNA, complete cds. 9/11 None
ZK970.1a.2 ENSEMBL cdna:known chromosome:CEL140:II:10283186:10295604:-1 gene:ZK970.1 9/11 None
ZK970.1a.1 ENSEMBL cdna:known chromosome:CEL140:II:10283389:10295674:-1 gene:ZK970.1 9/11 None
ZK970.1b ENSEMBL cdna:known chromosome:CEL140:II:10283974:10288847:-1 gene:ZK970.1 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10283970-10287754(-) 99.92 99.92
Public Domain and Genome References
Ensembl ZK970.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA88893 Hypothetical protein ZK970.1a [Caenorhabditis elegans] emb|CAA88892.3| Hypothetical protein ZK970.1a [Caenorhabditis elegans] ref|NP_001022539.1| ZK970.1a [Caenorhabditis elegans] 0.0
blast CAE48829 Hypothetical protein ZK970.1b [Caenorhabditis elegans] emb|CAE48845.1| Hypothetical protein ZK970.1b [Caenorhabditis elegans] ref|NP_001022540.1| ZK970.1b [Caenorhabditis elegans] 0.0
blast CAE72917 Hypothetical protein CBG20236 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 2.3E-7
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 9.1E-41
Pfam IPR008753 EMBL-EBI Peptidase M13 2.3E-7
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 9.1E-41
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022539.1 2 41-63,216-238
NP_001022540.1 1 74-96
ZK970.1a.2 2 41-63,216-238
ZK970.1a.1 2 41-63,216-238
ZK970.1b 1 74-96
Sequence
Target Sequence 
>C. ELEGANS:190720_AT
agtgcagattccattcgattcgttaagactaccatatgcagatgaacatcaattagattt
cgccaactacgcaggaatcggaacaatcattggacacgagcttacccatgcatttgatgg
acaaggtaaacttcacggcccaactggaaaccttggcgtttggtggagtaccgattcaat
tcgagagttcaaagctcgcgagcaatgtttcgtcaaacaatacgcaggattaatggattc
acatgacgtaagttcctggctgcgtccatccacactaattgcgtgtttacttcagatgaa
tgctgctaaggaaggtctctacgaaaacattgccgatcaagttggacttaaagtggctta
cgaggcatggaagtctaacggaaatcagaacagcggacggatgcctggattagaaaagta
ttctcaagatcagcttttctttttggcctacactcaaggatggtgtgctctcagatcaaa
gagctataagttacaaccacatatggaagagagaatacgaatgcttggctccctacaaaa
ctctccagagttcgcgtcagcgtggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTGCAGATTCCATTCGATTCGTTA 29 61 1758 Antisense
ATTTCGCCAACTACGCAGGAATCGG 661 15 1814 Antisense
ACACGAGCTTACCCATGCATTTGAT 599 109 1851 Antisense
CACGGCCCAACTGGAAACCTTGGCG 326 197 1891 Antisense
AAGCTCGCGAGCAATGTTTCGTCAA 177 151 1949 Antisense
TGACGTAAGTTCCTGGCTGCGTCCA 57 571 2001 Antisense
GCGTCCATCCACACTAATTGCGTGT 279 291 2019 Antisense
GAACAGCGGACGGATGCCTGGATTA 46 353 2145 Antisense
AAGATCAGCTTTTCTTTTTGGCCTA 455 155 2183 Antisense
AGAATACGAATGCTTGGCTCCCTAC 450 75 2269 Antisense
CTCCAGAGTTCGCGTCAGCGTGGAA 638 235 2300 Antisense
Sequence Source Affymetrix Proprietary Database