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[190717_s_at] serine/threonine kinase

Description Y38H8A.4 /REP_DB=WormBase Gene ID /WP=CE18366 /TR=O62424 /GB=CAA16343.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 98      4grp_total > 15      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 48.2
(-0.9)
A 		20.9
(-3)
A 		98.3
(3)
A 		63.3
(0.3)
A 		77
B 17.1
(-1.9)
A 		18.9
(-1.6)
A 		8.8
(-3)
A 		52.4
(3)
A 		44
C 80.3
(3)
A 		67.2
(1.8)
A 		14.3
(-3)
A 		15.5
(-2.9)
A 		66
avr 48.5
(3) 		35.7
(-3) 		40.5
(-0.8) 		43.7
(0.8) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 52.9
(-2.3)
A 		42.4
(-2.6)
A 		31.9
(-3)
A 		44.5
(-2.6)
A 		117.9
(-0.1)
A 		210.9
(3)
P 		179
B 53.6
(-1.7)
A 		18.2
(-2.9)
A 		16.3
(-3)
A 		61.9
(-1.4)
A 		142.9
(1.5)
P 		183.9
(3)
P 		168
C 8.2
(-3)
A 		62.5
(-0.6)
A 		49.4
(-1.2)
A 		81.9
(0.3)
A 		143.5
(3)
P 		137.6
(2.7)
P 		135
avr 38.2
(-2.8) 		41
(-2.6) 		32.5
(-3) 		62.8
(-1.7) 		134.8
(1.2) 		177.5
(3) 		145
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 17.5
(0.7)
A 		4.5
(-3)
A 		25.7
(3)
A 		9.3
(-1.6)
A 		21
B 5.5
(-0.7)
A 		4.7
(-1.2)
A 		2
(-3)
A 		11.2
(3)
A 		9
C 26.6
(3)
A 		19.3
(1.2)
A 		5
(-2.4)
A 		2.6
(-3)
A 		24
avr 16.5
(3) 		9.5
(-1.8) 		10.9
(-0.8) 		7.7
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 9.8
(-2.6)
A 		15.1
(-1.9)
A 		7
(-3)
A 		11.3
(-2.4)
A 		35.6
(0.9)
A 		51.4
(3)
P 		44
B 18.6
(-1.6)
A 		6.1
(-2.8)
A 		4
(-3)
A 		16.4
(-1.8)
A 		58.1
(2)
P 		68.6
(3)
P 		65
C 3
(-3)
A 		19.1
(-0.8)
A 		12.6
(-1.7)
A 		16.5
(-1.1)
A 		46.4
(3)
P 		46.1
(3)
P 		43
avr 10.5
(-2.7) 		13.4
(-2.3) 		7.9
(-3) 		14.7
(-2.1) 		46.7
(1.9) 		55.4
(3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190717_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8484
Sequence Type Exemplar sequence
Representative Public ID Y38H8A.4 NCBI
Target Description Y38H8A.4 /REP_DB=WormBase Gene ID /WP=CE18366 /TR=O62424 /GB=CAA16343.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070194(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070194 NCBI Caenorhabditis elegans Y38H8A.4 (Y38H8A.4) mRNA, complete cds. 11/11 None
SNAP00000011903 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13470602:13472211:-1 11/11 None
GENEFINDER00000011909 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13470602:13472211:-1 11/11 None
Y38H8A.4 ENSEMBL cdna:known chromosome:CEL140:IV:13470601:13472211:-1 gene:Y38H8A.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026824 2/11 Cross Hyb Matching Probes None
SNAP00000035737 2/11 Cross Hyb Matching Probes None
GENEFINDER00000035750 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5159761-5161109(-) 91.16 91.16
chrIV:13470607-13472217(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase
Ensembl Y38H8A.4
Entrez Gene 178314 Entrez gene
SwissProt O62424 EMBL-EBI
WormBase CE18366 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584770_AT similar to serine/threonine kinase 22B (spermiogenesis associated) (predicted) cfa
CANINE_2:CFA.8526.1.A1_AT similar to serine/threonine kinase 22B (spermiogenesis associated) (predicted) cfa
CANINE_2:CFA.8526.1.A1_S_AT similar to serine/threonine kinase 22B (spermiogenesis associated) (predicted) cfa
CANINE_2:CFAAFFX.22803.1.S1_S_AT similar to serine/threonine kinase 22B (spermiogenesis associated) (predicted) cfa
HG-U133_PLUS_2:214371_AT testis-specific serine kinase 2 hs
HG-U133A_2:214371_AT testis-specific serine kinase 2 hs
HG-U133A:214371_AT testis-specific serine kinase 2 hs
HU35KSUBA:L77564_AT testis-specific serine kinase 2 hs
U133_X3P:HS.250168.0.A1_3P_AT testis-specific serine kinase 2 hs
U133_X3P:HS.103978.0.S1_3P_X_AT testis-specific serine kinase 2 hs
U133_X3P:HS.103978.0.S1_3P_AT testis-specific serine kinase 2 hs
HG-U133A_2:217275_AT testis-specific serine kinase 2 hs
HG-U133_PLUS_2:217275_AT testis-specific serine kinase 2 hs
HG-U133A:217275_AT testis-specific serine kinase 2 hs
HG-U95AV2:39534_AT testis-specific serine kinase 2 hs
MOUSE430_2:1449489_AT testis-specific serine kinase 2 mm
MOUSE430A_2:1449489_AT testis-specific serine kinase 2 mm
MOE430A:1449489_AT testis-specific serine kinase 2 mm
MU11KSUBB:MSA.34904.0_F_AT testis-specific serine kinase 2 mm
MG-U74AV2:102846_AT testis-specific serine kinase 2 mm
MU19KSUBA:TC16472_AT Testis-specific serine kinase 2 (Tssk2), mRNA mm
MU19KSUBA:TC17091_AT Testis-specific serine kinase 2 (Tssk2), mRNA mm
RAE230B:1393744_AT serine/threonine kinase 22B (spermiogenesis associated) (predicted) rn
RAT230_2:1393744_AT serine/threonine kinase 22B (spermiogenesis associated) (predicted) rn
RAE230A:1376981_AT Serine/threonine kinase 22B (spermiogenesis associated) (predicted) rn
RAT230_2:1376981_AT Serine/threonine kinase 22B (spermiogenesis associated) (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA16343 Hypothetical protein Y38H8A.4 [Caenorhabditis elegans] ref|NP_502595.1| Y38H8A.4 [Caenorhabditis elegans] 1.0E-178
blast NP_001021995 C27D6.11 [Caenorhabditis elegans] gb|AAY55860.1| Hypothetical protein C27D6.11 [Caenorhabditis elegans] 1.0E-163
blast CAE66819 Hypothetical protein CBG12184 [Caenorhabditis briggsae] 1.0E-161
Protein Families
Method ID Description E-Value
hanks 2.2.11 CaMK Group; CaMK II KIN1/SNF1/Nim1; YKL453 8.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.2E-70
Sequence
Target Sequence 
>C. ELEGANS:190717_S_AT
agagaacgcgaagttcaccttccgtcagttcatcgcagctctcatgcatctccaatcata
taacattgtgcaccgtgacctcaagtgcgagaacatctttcttgacaagtacgagaacgt
caaactcggagattttggcttctcgcgtatcctgaaacctggagagaagtctggaacatt
ctgcggatctcgtgcgtatgttgctccggagattctgcgcggaagagaatattcgggaaa
tgcggttgatgtctggagtaccggagtgattttgtacattatgttggttggaaccatgcc
gtttgatgatcgcgatccgacgagaatgattgagagacaactggctcataaaattaaatt
cggaaaaacctgtacagcctccattcattccaaggctctgattttggagattcttcagcc
tcatgctccaaatcgaccaacttac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGAACGCGAAGTTCACCTTCCGT 520 69 417 Antisense
GCTCTCATGCATCTCCAATCATATA 630 299 454 Antisense
AACATTGTGCACCGTGACCTCAAGT 272 137 478 Antisense
CTTCTCGCGTATCCTGAAACCTGGA 490 219 555 Antisense
AGTCTGGAACATTCTGCGGATCTCG 242 49 584 Antisense
CGGATCTCGTGCGTATGTTGCTCCG 593 243 600 Antisense
GTTGCTCCGGAGATTCTGCGCGGAA 204 439 616 Antisense
GGAACCATGCCGTTTGATGATCGCG 622 529 706 Antisense
GGAAAAACCTGTACAGCCTCCATTC 80 529 778 Antisense
TGGAGATTCTTCAGCCTCATGCTCC 563 549 821 Antisense
TCATGCTCCAAATCGACCAACTTAC 435 619 837 Antisense
Sequence Source Affymetrix Proprietary Database