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[190716_at] nanos protein

Description R03D7.7 /REP_DB=WormBase Gene ID /WP=CE01614 /GEN=nos-1 /TR=Q09597 /GB=CAA86860.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=nanos protein
Cluster 6grp_total > 88      4grp_total > 07      6grp_cgc > 59      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 102.2
(-2.8)
A 		128.4
(0.2)
A 		152.6
(3)
A 		100.9
(-3)
A 		52
B 78
(-0.5)
A 		20.7
(-3)
A 		86.5
(-0.1)
A 		157.6
(3)
A 		137
C 81.9
(3)
A 		12.8
(-3)
A 		67.6
(1.8)
A 		29.5
(-1.5)
A 		69
avr 87.4
(1.2) 		54
(-3) 		102.2
(3) 		96
(2.2) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 695
1 2 3 4 5 6 range
A 104.1
(-2)
A 		8
(-3)
A 		17.1
(-2.9)
A 		36.3
(-2.7)
A 		356.1
(0.5)
P 		610.8
(3)
P 		603
B 11.6
(-3)
A 		25.6
(-2.9)
A 		92.9
(-2.3)
A 		164.2
(-1.7)
A 		334.1
(-0.2)
P 		703.1
(3)
P 		692
C 14.8
(-3)
A 		124.6
(-1.9)
A 		94
(-2.2)
A 		64.7
(-2.5)
A 		331.4
(0.1)
P 		623.2
(3)
P 		608
avr 43.5
(-3) 		52.7
(-2.9) 		68
(-2.8) 		88.4
(-2.6) 		340.5
(-0) 		645.7
(3) 		602
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 37.2
(2.4)
A 		27.9
(0.1)
A 		39.9
(3)
A 		14.8
(-3)
A 		25
B 24.9
(1.2)
A 		5.1
(-3)
A 		20
(0.1)
A 		33.6
(3)
A 		29
C 27.1
(3)
A 		3.7
(-3)
A 		23.6
(2.1)
A 		5
(-2.7)
A 		23
avr 29.7
(3) 		12.2
(-3) 		27.8
(2.3) 		17.8
(-1.1) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 259
1 2 3 4 5 6 range
A 19.2
(-2.3)
A 		2.8
(-3)
A 		3.7
(-3)
A 		9.2
(-2.7)
A 		107.4
(1.3)
P 		148.7
(3)
P 		146
B 4
(-3)
A 		8.5
(-2.9)
A 		22.6
(-2.6)
A 		43.5
(-2.1)
A 		135.8
(0.1)
P 		262.2
(3)
P 		258
C 5.5
(-3)
A 		38
(-2)
A 		23.9
(-2.5)
A 		13
(-2.8)
A 		107.2
(0)
P 		208.6
(3)
P 		203
avr 9.6
(-3) 		16.4
(-2.8) 		16.7
(-2.8) 		21.9
(-2.6) 		116.8
(0.3) 		206.5
(3) 		197
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190716_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3303
Sequence Type Exemplar sequence
Representative Public ID R03D7.7 NCBI
Target Description R03D7.7 /REP_DB=WormBase Gene ID /WP=CE01614 /GEN=nos-1 /TR=Q09597 /GB=CAA86860.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=nanos protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063957(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063957 NCBI Caenorhabditis elegans NanOS related family member (nos-1) (nos-1) mRNA, complete cds. 11/11 None
SNAP00000040572 ENSEMBL cdna:SNAP chromosome:CEL140:II:10951790:10953076:-1 11/11 None
GENEFINDER00000040579 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10951790:10953052:-1 11/11 None
R03D7.7 ENSEMBL cdna:known chromosome:CEL140:II:10951790:10953076:-1 gene:R03D7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10951786-10953073(-) 100.0 100.0
Public Domain and Genome References
Gene Title nanos protein
Gene Symbol nos-1
Ensembl R03D7.7
Entrez Gene 191735 Entrez gene
SwissProt Q09597 EMBL-EBI
WormBase CE01614 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6445 regulation of translation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86860 Hypothetical protein R03D7.7 [Caenorhabditis elegans] ref|NP_496358.1| NanOS related family member (nos-1) [Caenorhabditis elegans] 1.0E-177
blast CAA86860 Hypothetical protein R03D7.7 [Caenorhabditis elegans] ref|NP_496358.1| NanOS related family member (nos-1) [Caenorhabditis elegans] 1.0E-172
blast CAA86860 Hypothetical protein R03D7.7 [Caenorhabditis elegans] ref|NP_496358.1| NanOS related family member (nos-1) [Caenorhabditis elegans] 1.0E-150
blast CAE73463 Hypothetical protein CBG20913 [Caenorhabditis briggsae] 2.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR008705 EMBL-EBI Nanos RNA-binding 1.4E-25
Pfam IPR008705 EMBL-EBI Nanos RNA-binding 1.4E-25
Pfam IPR008705 EMBL-EBI Nanos RNA-binding 1.4E-25
Sequence
Target Sequence 
>C. ELEGANS:190716_AT
ggtttcgctggttttgggatgtccgtttggagccatcggacaggaacgccggatgtctca
agattttccgttccagagaagaagcttcgatatggtttcgaacaggacttccacctcaga
atcttcaagctgtagcagcactgacaatacttacaagaacgccggaaagttcctttcact
gcaggaatatttggagcaaaacaatcttctgccaccatcgactatgccgtttggagagaa
ctctggatttcacgggtacaacccccaaatgaatgtacataataattctactcgtaatgg
aaatgttgagaatcgagagcagcgaaacaactccacttcgccaccgagaggaaatcctcc
acatccactttgctgctgcttctgcttcggaaccgcttccgaatttgcgagacttcacac
tttgccagctccacggaaagatgatcgtggaccatggtccgatcactgctcgaagaaaag
gggaagagttgtatgcccaaaacttcgctcaatggtctgtggaatctgtggagccactgg
cgataacgcacacac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTTCGCTGGTTTTGGGATGTCCG 208 505 357 Antisense
AGGAACGCCGGATGTCTCAAGATTT 350 53 398 Antisense
TTCGAACAGGACTTCCACCTCAGAA 461 695 453 Antisense
AGCAAAACAATCTTCTGCCACCATC 7 77 551 Antisense
CCACCATCGACTATGCCGTTTGGAG 557 273 568 Antisense
GGAACCGCTTCCGAATTTGCGAGAC 266 529 745 Antisense
AATTTGCGAGACTTCACACTTTGCC 273 175 758 Antisense
CACTTTGCCAGCTCCACGGAAAGAT 435 197 774 Antisense
CATGGTCCGATCACTGCTCGAAGAA 517 209 809 Antisense
GTTGTATGCCCAAAACTTCGCTCAA 537 437 844 Antisense
GAGCCACTGGCGATAACGCACACAC 588 383 887 Antisense
Sequence Source Affymetrix Proprietary Database