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[190713_at] Environmental stress

Description F22B7.4 /REP_DB=WormBase Gene ID /WP=CE00157 /TR=SW:P34407 /GB=AAA65461.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Environmental stress
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 33      4grp_highest_8_8 > 04      4grp_high_8_8 > 37      6grp_highest_nonorm > 33      6grp_total > 11      4grp_total > 42      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 467
1 2 3 4 range
A 68.9
(-3)
P 		294.1
(3)
P 		158.1
(-0.6)
P 		193.8
(0.3)
P 		225
B 66.7
(-3)
P 		463.6
(3)
P 		139.3
(-1.9)
P 		141
(-1.9)
P 		397
C 81.9
(-3)
P 		533.3
(3)
P 		146.4
(-2.1)
P 		266.6
(-0.5)
P 		451
avr 72.5
(-3) 		430.3
(3) 		147.9
(-1.7) 		200.5
(-0.9) 		358

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2089
1 2 3 4 5 6 range
A 938.2
(-2.4)
P 		2162.6
(1.6)
P 		2592.4
(3)
P 		1417.3
(-0.8)
P 		881.5
(-2.5)
P 		740.4
(-3)
P 		1852
B 1282.5
(-2)
P 		2485.8
(3)
P 		1330.3
(-1.8)
P 		1191
(-2.3)
P 		1301.7
(-1.9)
P 		1030.7
(-3)
P 		1455
C 1562.4
(-0.6)
P 		2805.1
(3)
P 		1628.9
(-0.4)
P 		1293.5
(-1.3)
P 		1339.1
(-1.2)
P 		716.5
(-3)
P 		2089
avr 1261
(-1.4) 		2484.5
(3) 		1850.5
(0.7) 		1300.6
(-1.3) 		1174.1
(-1.7) 		829.2
(-3) 		1655
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 132
1 2 3 4 range
A 25.1
(-3)
P 		63.8
(3)
P 		41.3
(-0.5)
P 		28.4
(-2.5)
P 		39
B 21.3
(-3)
P 		114.5
(3)
P 		32.3
(-2.3)
P 		30.1
(-2.4)
P 		93
C 27.1
(-3)
P 		153.3
(3)
P 		51
(-1.9)
P 		44.9
(-2.2)
P 		126
avr 24.5
(-3) 		110.5
(3) 		41.5
(-1.8) 		34.5
(-2.3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 683
1 2 3 4 5 6 range
A 172.9
(-3)
P 		769.1
(3)
P 		564.6
(0.9)
P 		359.5
(-1.1)
P 		266
(-2.1)
P 		180.3
(-2.9)
P 		596
B 446.2
(-1.5)
P 		827.4
(3)
P 		323
(-2.9)
P 		315.4
(-3)
P 		528.9
(-0.5)
P 		384.3
(-2.2)
P 		512
C 579.1
(0.3)
P 		855.9
(3)
P 		414.6
(-1.3)
P 		260.1
(-2.8)
P 		433.3
(-1.1)
P 		239.9
(-3)
P 		616
avr 399.4
(-1.6) 		817.5
(3) 		434.1
(-1.2) 		311.7
(-2.5) 		409.4
(-1.5) 		268.2
(-3) 		549
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190713_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6326
Sequence Type Exemplar sequence
Representative Public ID F22B7.4 NCBI
Target Description F22B7.4 /REP_DB=WormBase Gene ID /WP=CE00157 /TR=SW:P34407 /GB=AAA65461.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Environmental stress
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066503(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066503 NCBI Caenorhabditis elegans F22B7.4 (F22B7.4) mRNA, complete cds. 11/11 None
F22B7.4 ENSEMBL cdna:known chromosome:CEL140:III:8639694:8639931:1 gene:F22B7.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000003833 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8639688-8639926(+) 100.0 100.0
Public Domain and Genome References
Gene Title Environmental stress
Ensembl F22B7.4
Entrez Gene 184814 Entrez gene
SwissProt P34407 EMBL-EBI
WormBase CE00157 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA65461 Hypothetical protein F22B7.4 [Caenorhabditis elegans] ref|NP_498904.1| F22B7.4 [Caenorhabditis elegans] sp|P34407|YLW4_CAEEL Hypothetical protein F22B7.4 in chromosome III 1.0E-12
blast CAE75021 Hypothetical protein CBG22925 [Caenorhabditis briggsae] 1.0E-9
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498904.1 1 7-29
F22B7.4 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:190713_AT
aatcttccaaattcttgtgatcttcgccattttgtcagcattgcaagtgaatggattcct
ctttccaacttattcgtcaggatatgactacgattgttatggatacggtaacaatggtta
cggtaatggtggatacggctat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCTTCCAAATTCTTGTGATCTTC 197 171 21 Antisense
TCCAAATTCTTGTGATCTTCGCCAT 67 589 26 Antisense
TGTGATCTTCGCCATTTTGTCAGCA 37 557 36 Antisense
TCTTCGCCATTTTGTCAGCATTGCA 538 617 41 Antisense
CGCCATTTTGTCAGCATTGCAAGTG 682 257 45 Antisense
GCAAGTGAATGGATTCCTCTTTCCA 336 321 63 Antisense
GAATGGATTCCTCTTTCCAACTTAT 600 323 69 Antisense
TCCTCTTTCCAACTTATTCGTCAGG 132 593 77 Antisense
GTCAGGATATGACTACGATTGTTAT 189 467 96 Antisense
GACTACGATTGTTATGGATACGGTA 64 373 106 Antisense
TTACGGTAATGGTGGATACGGCTAT 125 1 138 Antisense
Sequence Source Affymetrix Proprietary Database