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[190707_at] Major intrinsic protein

Description Y69E1A.7 /REP_DB=WormBase Gene ID /WP=CE22814 /TR=Q9XW36 /GB=CAA22259.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Major intrinsic protein
Cluster 6grp_total > 97      4grp_total > 02      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 259
1 2 3 4 range
A 18.5
(-3)
A 		42.3
(-2.4)
A 		48.8
(-2.2)
A 		255
(3)
P 		237
B 57.1
(-1.4)
A 		5.9
(-3)
A 		9.3
(-2.9)
A 		198.7
(3)
P 		193
C 26.5
(-3)
A 		63.2
(-2.1)
A 		29
(-2.9)
A 		264.6
(3)
P 		238
avr 34
(-2.9) 		37.1
(-2.8) 		29
(-3) 		239.4
(3) 		210

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 165
1 2 3 4 5 6 range
A 22.2
(-2.5)
A 		7.7
(-3)
A 		45
(-1.6)
A 		28.1
(-2.2)
A 		76.9
(-0.4)
A 		167.1
(3)
A 		159
B 30.5
(-1.5)
A 		2.5
(-3)
A 		20.7
(-2)
A 		26.6
(-1.7)
A 		78
(1.1)
A 		111.9
(3)
P 		109
C 39.6
(-1)
A 		57.8
(0.1)
A 		6.5
(-3)
A 		64.6
(0.5)
A 		105.4
(3)
A 		103.6
(2.9)
P 		99
avr 30.8
(-2.5) 		22.7
(-3) 		24.1
(-2.9) 		39.8
(-2) 		86.8
(0.7) 		127.5
(3) 		105
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 6.8
(-3)
A 		9.2
(-2.5)
A 		12.8
(-1.8)
A 		37.3
(3)
P 		31
B 18.2
(-0.5)
A 		1.5
(-3)
A 		2.1
(-2.9)
A 		42.3
(3)
P 		41
C 8.8
(-3)
A 		18.2
(-1.4)
A 		10.1
(-2.8)
A 		44.6
(3)
P 		36
avr 11.3
(-2.5) 		9.6
(-2.8) 		8.3
(-3) 		41.4
(3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 4.1
(-2.8)
A 		2.7
(-3)
A 		9.8
(-1.9)
A 		7.1
(-2.3)
A 		23.2
(0.2)
A 		40.7
(3)
A 		38
B 10.6
(-1.6)
A 		0.8
(-3)
A 		5
(-2.4)
A 		7
(-2.1)
A 		31.7
(1.5)
A 		41.7
(3)
P 		41
C 14.7
(-0.6)
A 		17.6
(-0.1)
A 		1.7
(-3)
A 		13
(-0.9)
A 		34.1
(2.9)
A 		34.7
(3)
P 		33
avr 9.8
(-2.2) 		7
(-2.7) 		5.5
(-3) 		9
(-2.4) 		29.7
(1.3) 		39
(3) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190707_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10308
Sequence Type Exemplar sequence
Representative Public ID Y69E1A.7 NCBI
Target Description Y69E1A.7 /REP_DB=WormBase Gene ID /WP=CE22814 /TR=Q9XW36 /GB=CAA22259.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Major intrinsic protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069643(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069643 NCBI Caenorhabditis elegans AQuaPorin or aquaglyceroporin related family member (aqp-3) (aqp-3) mRNA, complete cds. 11/11 None
SNAP00000037377 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10961325:10963785:-1 11/11 None
GENEFINDER00000037386 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10961325:10963785:-1 11/11 None
Y69E1A.7 ENSEMBL cdna:known chromosome:CEL140:IV:10961325:10963785:-1 gene:Y69E1A.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10961330-10963791(-) 100.0 100.0
Public Domain and Genome References
Gene Title Major intrinsic protein
Gene Symbol aqp-3
Ensembl Y69E1A.7
Entrez Gene 190553 Entrez gene
SwissProt Q9XW36 EMBL-EBI
WormBase CE22814 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256398_AT major intrinsic family protein / MIP family protein at
CANINE_2:CFAAFFX.25331.1.S1_AT similar to aquaporin 9 cfa
CHICKEN:GGA.12485.1.S1_AT aquaporin 9 gga
CHICKEN:GGA.12485.2.S1_A_AT aquaporin 9 gga
HG-U95AV2:34435_AT aquaporin 9 hs
HG-U133_PLUS_2:205568_AT aquaporin 9 hs
HG-U133A:205568_AT aquaporin 9 hs
HG-FOCUS:205568_AT aquaporin 9 hs
HG-U133A_2:205568_AT aquaporin 9 hs
U133_X3P:G11038652_3P_AT aquaporin 9 hs
HG-U95C:63266_R_AT Aquaporin 9 hs
HU35KSUBA:AA381159_AT Aquaporin 9 hs
HU35KSUBC:RC_N24949_AT Aquaporin 9 hs
MOUSE430_2:1421605_A_AT aquaporin 9 mm
MOE430A:1421605_A_AT aquaporin 9 mm
MOUSE430A_2:1421605_A_AT aquaporin 9 mm
MG-U74AV2:104328_AT aquaporin 9 mm
MOE430A:1424011_AT aquaporin 9 mm
MOUSE430A_2:1424011_AT aquaporin 9 mm
MOUSE430_2:1424011_AT aquaporin 9 mm
MU19KSUBC:TC41321_AT Aquaporin 9, mRNA (cDNA clone MGC:35844 IMAGE:5096952) mm
MU19KSUBC:TC41321_G_AT Aquaporin 9, mRNA (cDNA clone MGC:35844 IMAGE:5096952) mm
RG-U34A:AB013112_S_AT aquaporin 9 rn
RAE230A:1368621_AT aquaporin 9 rn
RAT230_2:1368621_AT aquaporin 9 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA22259 Hypothetical protein Y69E1A.7 [Caenorhabditis elegans] ref|NP_502044.1| AQuaPorin or aquaglyceroporin related family member (aqp-3) [Caenorhabditis elegans] 0.0
blast CAE61966 Hypothetical protein CBG05966 [Caenorhabditis briggsae] 0.0
blast CAA84633 Hypothetical protein C01G6.1a [Caenorhabditis elegans] ref|NP_495972.1| AQuaPorin or aquaglyceroporin related family member (aqp-2) [Caenorhabditis elegans] 2.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR000425 EMBL-EBI Major intrinsic protein 4.9E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502044.1 6 122-144,154-176,196-218,254-276,289-311,340-362
Y69E1A.7 6 122-144,154-176,196-218,254-276,289-311,340-362
SNAP00000037377 6 122-144,154-176,196-218,254-276,289-311,340-362
GENEFINDER00000037386 6 122-144,154-176,196-218,254-276,289-311,340-362
Sequence
Target Sequence 
>C. ELEGANS:190707_AT
atcgtacagtaaccggaccaacagcaactgcatctatttttgcaacctatcctggaccct
tcttaggaactttcaatgcaattgtggatcaaatcgccggaactctagtactttgtcttg
gagtagcagccatcacagatcgtcgaaacggaataccagccttcctccaaccagcatgga
ttggagcacttctcgcattcctcggaatgtcacttgctttaaatgctggttatgcaatca
atccagcacgtgattttgcacctcgtctcttcaatttatgtgcaggttacggatgggaag
ttttcagttatagaaattataaatggttctggattccaattatttgcccaatgattggtg
gcgttcttggtgcatggctttacgaatttttcattggtttccatattcaagatgaagatg
ctgtctcattagattccgaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGTACAGTAACCGGACCAACAGC 672 33 707 Antisense
TATTTTTGCAACCTATCCTGGACCC 98 663 741 Antisense
GGACCCTTCTTAGGAACTTTCAATG 22 489 760 Antisense
ATCGCCGGAACTCTAGTACTTTGTC 219 1 799 Antisense
AGCAGCCATCACAGATCGTCGAAAC 242 77 831 Antisense
CCTTCCTCCAACCAGCATGGATTGG 540 269 866 Antisense
ATTCCTCGGAATGTCACTTGCTTTA 575 7 903 Antisense
ATCCAGCACGTGATTTTGCACCTCG 600 39 947 Antisense
ACCTCGTCTCTTCAATTTATGTGCA 121 89 966 Antisense
GCGTTCTTGGTGCATGGCTTTACGA 679 289 1067 Antisense
GATGCTGTCTCATTAGATTCCGAAA 178 415 1123 Antisense
Sequence Source Affymetrix Proprietary Database