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[190706_at] glutathione S-transferase

Description F37F2.3 /REP_DB=WormBase Gene ID /WP=CE17804 /TR=O61750 /GB=AAC16998.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=glutathione transferase
Cluster 6grp_total > 08      4grp_total > 47      6grp_cgc > 06      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 14.2
(3)
A 		3.7
(-2.9)
A 		3.6
(-3)
A 		4.4
(-2.5)
A 		11
B 7.9
(3)
A 		2.7
(-3)
A 		3.3
(-2.3)
A 		6.3
(1.2)
A 		5
C 15.8
(3)
A 		4.1
(-2.6)
A 		3.2
(-3)
A 		3.6
(-2.8)
A 		13
avr 12.6
(3) 		3.5
(-2.9) 		3.4
(-3) 		4.8
(-2.1) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 16
(3)
A 		4.1
(-2.6)
A 		6.2
(-1.6)
A 		3.2
(-3)
A 		6.4
(-1.5)
A 		9
(-0.3)
A 		13
B 9.5
(3)
A 		5.2
(-0.5)
A 		5.1
(-0.6)
A 		5.8
(-0)
A 		3.4
(-1.9)
A 		2.1
(-3)
A 		7
C 13.2
(3)
A 		4.4
(-2.1)
A 		3.4
(-2.7)
A 		4.4
(-2.1)
A 		2.8
(-3)
A 		7.1
(-0.5)
A 		10
avr 12.9
(3) 		4.6
(-2.7) 		4.9
(-2.5) 		4.5
(-2.8) 		4.2
(-3) 		6.1
(-1.7) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 5.2
(3)
A 		0.8
(-2.7)
A 		0.9
(-2.6)
A 		0.6
(-3)
A 		5
B 2.5
(3)
A 		0.7
(-3)
A 		0.8
(-2.7)
A 		1.3
(-1)
A 		2
C 5.2
(3)
A 		1.2
(-2.2)
A 		1.1
(-2.3)
A 		0.6
(-3)
A 		5
avr 4.3
(3) 		0.9
(-2.8) 		0.9
(-2.8) 		0.8
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 2.9
(3)
A 		1.5
(-1)
A 		1.3
(-1.6)
A 		0.8
(-3)
A 		1.9
(0.1)
A 		2.2
(1)
A 		2
B 3.3
(3)
A 		1.7
(-0.8)
A 		1.2
(-2)
A 		1.5
(-1.3)
A 		1.4
(-1.6)
A 		0.8
(-3)
A 		3
C 4.9
(3)
A 		1.3
(-2.4)
A 		0.9
(-3)
A 		0.9
(-3)
A 		0.9
(-3)
A 		2.4
(-0.8)
A 		4
avr 3.7
(3) 		1.5
(-2.1) 		1.1
(-3) 		1.1
(-3) 		1.4
(-2.3) 		1.8
(-1.4) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190706_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.257
Sequence Type Exemplar sequence
Representative Public ID F37F2.3 NCBI
Target Description F37F2.3 /REP_DB=WormBase Gene ID /WP=CE17804 /TR=O61750 /GB=AAC16998.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=glutathione transferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058456(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058456 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-25) (gst-25) mRNA, complete cds. 11/11 None
SNAP00000020127 ENSEMBL cdna:SNAP chromosome:CEL140:I:1451138:1453071:-1 11/11 None
GENEFINDER00000020135 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1451138:1453071:-1 11/11 None
F37F2.3 ENSEMBL cdna:known chromosome:CEL140:I:1451123:1453078:-1 gene:F37F2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1451137-1453071(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-25
Ensembl F37F2.3
Entrez Gene 185429 Entrez gene
SwissProt O61750 EMBL-EBI
WormBase CE17804 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4364 glutathione transferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC16998 Glutathione s-transferase protein 25 [Caenorhabditis elegans] ref|NP_490857.1| Glutathione S-Transferase family member (gst-25) [Caenorhabditis elegans] 1.0E-119
blast CAE62680 Hypothetical protein CBG06825 [Caenorhabditis briggsae] 3.0E-80
blast CAA78471 Hypothetical protein R107.7 [Caenorhabditis elegans] emb|CAA31979.1| unnamed protein product [Caenorhabditis elegans] ref|NP_499006.1| Glutathione S-Transferase family member (gst-1) [Caenorhabditis elegans] sp|P10299|GSTP1_CAEEL Glutathione S-transferase P (GST class-pi) 1.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 7.4E-11
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 3.9E-13
Sequence
Target Sequence 
>C. ELEGANS:190706_AT
ttggaacccgcggtatcggtgagccgattcgtatgcttctcacggaccaggaagtcgcat
tcattgatcatcgcttttcatgggagcaatgggcggatatcaaaccgacaatgatatttg
gctcctgtccttgtctgactatgggggataaggaaattgtgcaatctgggacaattataa
gacatctcgcaaggattcatggactcaacggaaccaacgaggaagaaaccatattcatcg
acatgttctacgagggagtccgcgatttgcacgccacctacgctaccatgatctaccaga
actatgaaaacggtcgccagccattcattcaagatgtcctgccagaacatctcgcacgtc
ttgagaagctcttccgcacctacgagaatggagagaactttgtagctggcgctaatatca
gttatgtggattacgtgctcttcgaggaacttgatattcagaagattttgtctcctacgg
cgctagacaggact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGAACCCGCGGTATCGGTGAGCC 545 681 35 Antisense
CGGTGAGCCGATTCGTATGCTTCTC 37 245 51 Antisense
GTCGCATTCATTGATCATCGCTTTT 399 475 88 Antisense
CAATGATATTTGGCTCCTGTCCTTG 568 181 143 Antisense
CTCCTGTCCTTGTCTGACTATGGGG 375 235 156 Antisense
CTACGCTACCATGATCTACCAGAAC 524 225 312 Antisense
GCCATTCATTCAAGATGTCCTGCCA 474 281 354 Antisense
CACGTCTTGAGAAGCTCTTCCGCAC 362 199 389 Antisense
GGAGAGAACTTTGTAGCTGGCGCTA 679 517 424 Antisense
ATGTGGATTACGTGCTCTTCGAGGA 510 49 458 Antisense
GTCTCCTACGGCGCTAGACAGGACT 476 443 504 Antisense
Sequence Source Affymetrix Proprietary Database