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[190697_at] calcium binding protein

Description K03E6.3 /REP_DB=WormBase Gene ID /WP=CE19523 /TR=Q9TZN4 /GB=AAC64979.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=calcium binding protein
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 56      4grp_high_8_8 > 20      6grp_highest_nonorm > 82      6grp_total > 47      4grp_total > 27      6grp_highest_sub > Cu      6grp_cgc > 27      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 417
1 2 3 4 range
A 538
(0.6)
P 		534.8
(0.5)
P 		396.3
(-3)
M 		635
(3)
P 		239
B 453.3
(-1.5)
P 		629.7
(1.2)
P 		356.2
(-3)
P 		744
(3)
P 		388
C 558.9
(-0.4)
P 		587
(0)
P 		394.9
(-3)
P 		773.5
(3)
P 		379
avr 516.7
(-0.6) 		583.8
(0.6) 		382.5
(-3) 		717.5
(3) 		335

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 324
1 2 3 4 5 6 range
A 448.7
(1.3)
P 		393.6
(-0.1)
P 		292
(-2.5)
P 		316.8
(-1.9)
P 		273.2
(-3)
P 		518.3
(3)
P 		245
B 481.8
(1.7)
P 		463
(1.3)
P 		229.5
(-3)
P 		341.6
(-0.9)
P 		288.9
(-1.9)
P 		553.4
(3)
P 		324
C 427.3
(1)
P 		501.7
(3)
P 		329.3
(-1.6)
P 		397.1
(0.2)
P 		277.4
(-3)
P 		470.3
(2.2)
P 		224
avr 452.6
(1.4) 		452.8
(1.4) 		283.6
(-2.9) 		351.8
(-1.2) 		279.8
(-3) 		514
(3) 		234
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 113
1 2 3 4 range
A 195.9
(3)
P 		116.1
(-1.7)
P 		103.6
(-2.4)
M 		93
(-3)
P 		103
B 144.5
(1.9)
P 		155.5
(2.8)
P 		82.5
(-3)
P 		158.5
(3)
P 		76
C 185
(3)
P 		168.8
(1.2)
P 		137.6
(-2.2)
P 		130.4
(-3)
P 		55
avr 175.1
(3) 		146.8
(0.5) 		107.9
(-3) 		127.3
(-1.3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 151
1 2 3 4 5 6 range
A 82.7
(-1.5)
P 		140
(3)
P 		63.6
(-3)
P 		80.3
(-1.7)
P 		82.4
(-1.5)
P 		126.2
(1.9)
P 		76
B 167.7
(1.5)
P 		154.1
(0.9)
P 		55.7
(-3)
P 		90.5
(-1.6)
P 		117.4
(-0.5)
P 		206.4
(3)
P 		151
C 158.4
(3)
P 		153.1
(2.6)
P 		83.8
(-2.7)
P 		79.9
(-3)
P 		89.8
(-2.2)
P 		157.4
(2.9)
P 		79
avr 136.3
(1.3) 		149.1
(2.1) 		67.7
(-3) 		83.6
(-2) 		96.5
(-1.2) 		163.3
(3) 		96
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190697_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18252
Sequence Type Exemplar sequence
Representative Public ID K03E6.3 NCBI
Target Description K03E6.3 /REP_DB=WormBase Gene ID /WP=CE19523 /TR=Q9TZN4 /GB=AAC64979.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=calcium binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075800(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075800 NCBI Caenorhabditis elegans Neuronal Calcium Sensor family member (ncs-3) (ncs-3) mRNA, complete cds. 10/11 None
SNAP00000010588 ENSEMBL cdna:SNAP chromosome:CEL140:X:1054211:1056686:1 10/11 None
GENEFINDER00000010599 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:1054211:1057077:1 9/11 None
K03E6.3 ENSEMBL cdna:known chromosome:CEL140:X:1054211:1056686:1 gene:K03E6.3 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_072683 1/11 Cross Hyb Matching Probes A 
SNAP00000035874 1/11 Cross Hyb Matching Probes None
C09H5.6 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:1054210-1056686(+) 100.0 100.0
Public Domain and Genome References
Gene Title calcium binding protein
Gene Symbol ncs-3
Ensembl K03E6.3
Entrez Gene 186953 Entrez gene
SwissProt Q95ZR9 EMBL-EBI
WormBase CE30288 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.17416.1.S1_S_AT similar to hippocalcin-like 1 cfa
DROSOPHILA_2:1636508_AT Neurocalcin dm
DROSGENOME1:143706_AT Neurocalcin dm
CHICKEN:GGA.3776.1.S1_AT hippocalcin-like 1 gga
MOUSE430A_2:1439113_AT hippocalcin-like 1 mm
MOE430A:1439113_AT hippocalcin-like 1 mm
MOUSE430_2:1439113_AT hippocalcin-like 1 mm
MU11KSUBB:MSA.14268.0_S_AT hippocalcin-like 1 mm
MG-U74AV2:103835_F_AT hippocalcin-like 1 mm
MOE430A:1448812_AT hippocalcin-like 1 mm
MOUSE430A_2:1448812_AT hippocalcin-like 1 mm
MOUSE430_2:1448812_AT hippocalcin-like 1 mm
RG-U34A:D13126_AT neural visinin-like Ca2+-binding protein type 3 rn
RG-U34C:RC_AI101921_AT neural visinin-like Ca2+-binding protein type 3 rn
RAE230A:1372179_AT neural visinin-like Ca2+-binding protein type 3 rn
RAT230_2:1372179_AT neural visinin-like Ca2+-binding protein type 3 rn
RAE230A:1369562_AT neural visinin-like Ca2+-binding protein type 3 rn
RAT230_2:1369562_AT neural visinin-like Ca2+-binding protein type 3 rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK82907 Neuronal calcium sensor family protein 3 [Caenorhabditis elegans] ref|NP_508201.2| Neuronal Calcium Sensor family member (ncs-3) [Caenorhabditis elegans] 1.0E-102
blast T33465 hypothetical protein K03E6.3 - Caenorhabditis elegans 3.0E-97
blast CAE63578 Hypothetical protein CBG08067 [Caenorhabditis briggsae] 2.0E-96
blast AAK82907 Neuronal calcium sensor family protein 3 [Caenorhabditis elegans] ref|NP_508201.2| Neuronal Calcium Sensor family member (ncs-3) [Caenorhabditis elegans] 6.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.1E-5
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 3.6E-7
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 7.6E-5
Sequence
Target Sequence 
>C. ELEGANS:190697_AT
agattttgtgagagactgtcccagtggagaactcaagatggaagaatttcaaggaatcta
caagcagttcttcccaaatggtgacccatcaaagtttgctgcttttgtgttcaatgtatt
tgatgacaatcacgatggtcacatttccttcagtgaattcattgctgcattgtcaatcac
atcccgtggcacattagacgagaagctagattgtaagtcgaaaaaaaaaatgcaaaaaga
gacattattgaaaatgtatgaaagtcactttgcaggggcattctcactatacgacgtgga
taaagacggctttattacaaaggatgaaatggctgatattgtcgatgcaatttatagcat
gattggaaatatgttggaactcccaaaagatgaggacacgcctcaaaagcgagtggaaaa
aatattcacaaatatggacaggaacctagacggtcaattgacaagagaagagttcaagga
aggctcaaaagcagacccttggattg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATTTTGTGAGAGACTGTCCCAGT 588 63 138 Antisense
GCAGTTCTTCCCAAATGGTGACCCA 58 311 201 Antisense
GGTGACCCATCAAAGTTTGCTGCTT 367 501 217 Antisense
AGTTTGCTGCTTTTGTGTTCAATGT 255 63 230 Antisense
TCACGATGGTCACATTTCCTTCAGT 179 625 267 Antisense
ATTGCTGCATTGTCAATCACATCCC 163 7 298 Antisense
ATCACATCCCGTGGCACATTAGACG 3 29 313 Antisense
AAAGTCACTTTGCAGGGGCATTCTC 700 119 398 Antisense
GGGCATTCTCACTATACGACGTGGA 195 493 413 Antisense
GAGGACACGCCTCAAAAGCGAGTGG 201 403 529 Antisense
GGCTCAAAAGCAGACCCTTGGATTG 55 505 619 Antisense
Sequence Source Affymetrix Proprietary Database