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[190691_at] alpha crystallin B chain

Description T22A3.2 /REP_DB=WormBase Gene ID /WP=CE13914 /TR=O01263 /GB=CAB03380.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=alpha crystallin B chain
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 43      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 52      6grp_total > 20      4grp_total > 49      6grp_highest_sub > 02_03      6grp_cgc > 72      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1281
1 2 3 4 range
A 139.2
(-3)
P 		1397.3
(3)
P 		1126.3
(1.7)
P 		374.9
(-1.9)
P 		1258
B 120.7
(-3)
P 		1123.7
(2.9)
P 		1142.8
(3)
P 		219.3
(-2.4)
P 		1022
C 116.7
(-3)
P 		1293
(3)
P 		1016.7
(1.6)
P 		273.1
(-2.2)
P 		1176
avr 125.5
(-3) 		1271.3
(3) 		1095.3
(2.1) 		289.1
(-2.1) 		1146

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 787
1 2 3 4 5 6 range
A 494.4
(-1.3)
P 		911.2
(1.9)
P 		1049.1
(3)
P 		458
(-1.6)
P 		308.8
(-2.7)
P 		272.2
(-3)
P 		777
B 396.2
(-2.3)
P 		1000.7
(3)
P 		902.7
(2.1)
P 		551.5
(-0.9)
P 		314.6
(-3)
P 		363.1
(-2.6)
P 		686
C 440.3
(-2.2)
P 		1059
(3)
P 		572.3
(-1.1)
P 		485.4
(-1.8)
P 		340.7
(-3)
P 		406.3
(-2.5)
P 		718
avr 443.6
(-1.9) 		990.3
(3) 		841.4
(1.7) 		498.3
(-1.4) 		321.4
(-3) 		347.2
(-2.8) 		669
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 333
1 2 3 4 range
A 50.7
(-3)
P 		303.3
(3)
P 		294.4
(2.8)
P 		54.9
(-2.9)
P 		253
B 38.5
(-3)
P 		277.5
(3)
P 		264.8
(2.7)
P 		46.7
(-2.8)
P 		239
C 38.6
(-3)
P 		371.7
(3)
P 		354.3
(2.7)
P 		46.1
(-2.9)
P 		333
avr 42.6
(-3) 		317.5
(3) 		304.5
(2.7) 		49.2
(-2.9) 		275

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 267
1 2 3 4 5 6 range
A 91.1
(-2.4)
P 		324.1
(3)
P 		228.5
(0.8)
P 		116.2
(-1.8)
P 		93.2
(-2.4)
P 		66.3
(-3)
P 		258
B 137.8
(-2.7)
P 		333.1
(3)
P 		219.2
(-0.3)
P 		146.1
(-2.5)
P 		127.8
(-3)
P 		135.4
(-2.8)
P 		205
C 163.2
(-1.3)
P 		323.1
(3)
P 		145.7
(-1.7)
P 		97.6
(-3)
P 		110.3
(-2.7)
P 		136
(-2)
P 		226
avr 130.7
(-2.4) 		326.8
(3) 		197.8
(-0.6) 		120
(-2.7) 		110.4
(-3) 		112.6
(-2.9) 		216
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190691_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1453
Sequence Type Exemplar sequence
Representative Public ID T22A3.2 NCBI
Target Description T22A3.2 /REP_DB=WormBase Gene ID /WP=CE13914 /TR=O01263 /GB=CAB03380.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=alpha crystallin B chain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060369(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060369 NCBI Caenorhabditis elegans Heat Shock Protein family member (hsp-12.1) (hsp-12.1) mRNA, complete cds. 11/11 None
SNAP00000000334 ENSEMBL cdna:SNAP chromosome:CEL140:I:10589811:10590729:-1 11/11 None
GENEFINDER00000000341 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10589811:10590729:-1 11/11 None
T22A3.2 ENSEMBL cdna:known chromosome:CEL140:I:10589811:10590729:-1 gene:T22A3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10589741-10590660(-) 100.0 100.0
Public Domain and Genome References
Gene Title alpha crystallin B chain
Gene Symbol hsp-12.1
Ensembl T22A3.2
Entrez Gene 188710 Entrez gene
SwissProt O01263 EMBL-EBI
WormBase CE13914 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03380 Hypothetical protein T22A3.2 [Caenorhabditis elegans] ref|NP_492770.1| Heat Shock Protein family member (hsp-12.1) [Caenorhabditis elegans] 4.0E-59
blast CAE58981 Hypothetical protein CBG02254 [Caenorhabditis briggsae] emb|CAE56912.1| Hypothetical protein CBG24755 [Caenorhabditis briggsae] 1.0E-54
blast AAA27953 Heat shock protein protein 12.2 [Caenorhabditis elegans] ref|NP_498776.1| C14B9.1 [Caenorhabditis elegans] sp|P34328|HSP10_CAEEL Heat shock protein Hsp-12.2 1.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 4.3E-12
Sequence
Target Sequence 
>C. ELEGANS:190691_AT
tccaattacaactgactcggcggcttcttgggattggccccttcagcataacgatggtgt
cgtgaaggtgaccaacacttcggagaaattcgaagttggacttgatgctggattctttgg
accaaatgatattgatgtgaaggtgaacggaatcgaaattatcattcatttgcgacatga
taatcgtccaaccgagtacggaatcgtcaatcgtgaggttcatcgtacttataaacttcc
agaagatgtagatccatcaactgttagatctcatctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCAATTACAACTGACTCGGCGGCT 569 583 24 Antisense
GCCCCTTCAGCATAACGATGGTGTC 459 285 60 Antisense
GATGGTGTCGTGAAGGTGACCAACA 245 407 76 Antisense
GGTGACCAACACTTCGGAGAAATTC 458 499 90 Antisense
GGACTTGATGCTGGATTCTTTGGAC 190 525 121 Antisense
TTGCGACATGATAATCGTCCAACCG 36 703 193 Antisense
GATAATCGTCCAACCGAGTACGGAA 173 421 202 Antisense
ACCGAGTACGGAATCGTCAATCGTG 358 37 214 Antisense
GTCAATCGTGAGGTTCATCGTACTT 440 463 229 Antisense
TCATCGTACTTATAAACTTCCAGAA 643 621 243 Antisense
TCCATCAACTGTTAGATCTCATCTC 67 591 276 Antisense
Sequence Source Affymetrix Proprietary Database