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[190683_at]

Description T19A5.2 /REP_DB=WormBase Gene ID /WP=CE07510 /TR=Q22553 /GB=AAC69038.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 39      4grp_high_8_8 > 49      6grp_highest_nonorm > 91      6grp_total > 38      4grp_total > 55      6grp_highest_sub > Cu      6grp_cgc > 08      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 237.3
(3)
P 		151.2
(-1.2)
P 		185.1
(0.5)
P 		114.2
(-3)
P 		123
B 225.6
(3)
P 		98.3
(-3)
A 		147.7
(-0.7)
P 		168.1
(0.3)
P 		127
C 230.2
(3)
P 		179.4
(0.6)
P 		197.4
(1.5)
P 		102.1
(-3)
P 		128
avr 231
(3) 		143
(-2.1) 		176.7
(-0.2) 		128.1
(-3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 357
1 2 3 4 5 6 range
A 138.4
(-2.3)
P 		216.9
(0.5)
P 		117.4
(-3)
P 		170.3
(-1.1)
P 		138.5
(-2.3)
P 		288.4
(3)
P 		171
B 236.4
(3)
P 		109.1
(-3)
P 		111.5
(-2.9)
P 		123.4
(-2.3)
P 		165.1
(-0.4)
P 		219
(2.2)
P 		127
C 454.6
(3)
P 		104.8
(-2.9)
P 		98.1
(-3)
P 		108
(-2.8)
P 		206.4
(-1.2)
P 		159.8
(-2)
P 		357
avr 276.5
(3) 		143.6
(-1.8) 		109
(-3) 		133.9
(-2.1) 		170
(-0.8) 		222.4
(1.1) 		168
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 86.4
(3)
P 		32.8
(-1.6)
P 		48.4
(-0.3)
P 		16.7
(-3)
P 		70
B 71.9
(3)
P 		24.3
(-3)
A 		34.2
(-1.8)
P 		35.8
(-1.6)
P 		48
C 76.2
(3)
P 		51.6
(0.5)
P 		68.8
(2.2)
P 		17.2
(-3)
P 		59
avr 78.2
(3) 		36.2
(-1.6) 		50.5
(-0) 		23.2
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 147
1 2 3 4 5 6 range
A 25.5
(-3)
P 		77.1
(3)
P 		25.6
(-3)
P 		43.2
(-0.9)
P 		41.8
(-1.1)
P 		70.2
(2.2)
P 		52
B 82.2
(3)
P 		36.3
(-2)
P 		27.1
(-3)
P 		32.7
(-2.4)
P 		67.1
(1.4)
P 		81.7
(2.9)
P 		55
C 168.5
(3)
P 		32
(-2.6)
P 		25
(-2.9)
P 		21.7
(-3)
P 		66.8
(-1.2)
P 		53.5
(-1.7)
P 		147
avr 92.1
(3) 		48.5
(-1) 		25.9
(-3) 		32.5
(-2.4) 		58.6
(-0) 		68.5
(0.9) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190683_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12177
Sequence Type Exemplar sequence
Representative Public ID T19A5.2 NCBI
Target Description T19A5.2 /REP_DB=WormBase Gene ID /WP=CE07510 /TR=Q22553 /GB=AAC69038.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072908(11), NM_072910(11), NM_072909(11), NM_001028970(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072908 NCBI Caenorhabditis elegans Germinal Center Kinase family member (gck-1) (gck-1) mRNA, complete cds. 11/11 None
NM_072910 NCBI Caenorhabditis elegans Germinal Center Kinase family member (gck-1) (gck-1) mRNA, complete cds. 11/11 None
NM_072909 NCBI Caenorhabditis elegans Germinal Center Kinase family member (gck-1) (gck-1) mRNA, complete cds. 11/11 None
NM_001028970 NCBI Caenorhabditis elegans Germinal Center Kinase family member (gck-1) (gck-1) mRNA, complete cds. 11/11 None
SNAP00000024600 ENSEMBL cdna:SNAP chromosome:CEL140:V:8954018:8958001:1 11/11 None
GENEFINDER00000024611 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8953336:8958001:1 11/11 None
T19A5.2a ENSEMBL cdna:known chromosome:CEL140:V:8950398:8958880:1 gene:T19A5.2 11/11 None
T19A5.2c ENSEMBL cdna:known chromosome:CEL140:V:8951623:8958880:1 gene:T19A5.2 11/11 None
T19A5.2b ENSEMBL cdna:known chromosome:CEL140:V:8951751:8958001:1 gene:T19A5.2 11/11 None
T19A5.2d ENSEMBL cdna:known chromosome:CEL140:V:8952138:8958880:1 gene:T19A5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8950398-8958002(+) 100.0 100.0
Public Domain and Genome References
Ensembl T19A5.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69038 Germinal center kinase family protein 1, isoform a [Caenorhabditis elegans] ref|NP_505309.1| Germinal Center Kinase family member (gck-1) [Caenorhabditis elegans] 0.0
blast AAT81184 Germinal center kinase family protein 1, isoform d [Caenorhabditis elegans] ref|NP_001024141.1| Germinal Center Kinase family member (gck-1) [Caenorhabditis elegans] 0.0
blast AAK77641 Germinal center kinase family protein 1, isoform b [Caenorhabditis elegans] ref|NP_505310.1| Germinal Center Kinase family member (gck-1) [Caenorhabditis elegans] 0.0
blast AAK77642 Germinal center kinase family protein 1, isoform c [Caenorhabditis elegans] ref|NP_505311.2| Germinal Center Kinase family member (gck-1) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-61
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-63
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-68
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-68
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 9.0E-69
hanks 2.1.10 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; PSK-H1 4.0E-70
hanks 2.1.5 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DmCamKII 3.0E-76
hanks 1.9.6 AGC group; AGC Other; SPK1 1.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-93
Pfam IPR000719 EMBL-EBI Protein kinase 7.8E-87
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-93
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-93
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-58
Pfam IPR000719 EMBL-EBI Protein kinase 3.8E-44
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-93
Sequence
Target Sequence 
>C. ELEGANS:190683_AT
caagtggacaacgatccggttctgcccaatcttgggaacttgaacgtggaaatcgaccta
tgagcgaacgggtatcatcgcaagtatccccgtcgaaatataaccaacacaggacatcat
cgagcaatggtgtgcaaggtggaagtggtggtcgtcgagaatacatcaatggatctggat
ctggattaaatggaaatagttctaatcagaatcattctgaatattcggatgccgtgcgac
aacgtggaccaggagggtccggtggtagacttgattatcgtgaaagccatgttccgacat
cgtcacaagaaaatctgaatcatggtagaatgtacgggtacggagcgccaccaccatcaa
gagaagctaataatgttccaatgcctcgtgtgaaaggtgccctcgattgtagtcttcttc
cggcaatcgaacatttgtctcgaacacgtcatgcgactgctgctctcgatcaattgagac
acgtttttcgagatgttgaagattcttgtcctggaatctgtaatgagttaatcgaagagt
tgatgcaaagaattgcagttcctcaggtgagccaaagtgacctcgatgctgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGTGGACAACGATCCGGTTCTGC 195 181 1352 Antisense
ATCGCAAGTATCCCCGTCGAAATAT 65 37 1428 Antisense
TATTCGGATGCCGTGCGACAACGTG 355 663 1573 Antisense
GTGAAAGCCATGTTCCGACATCGTC 474 481 1631 Antisense
GAATGTACGGGTACGGAGCGCCACC 11 331 1679 Antisense
AATAATGTTCCAATGCCTCGTGTGA 577 171 1720 Antisense
GTGAAAGGTGCCCTCGATTGTAGTC 132 481 1741 Antisense
GATTGTAGTCTTCTTCCGGCAATCG 448 435 1756 Antisense
TGTCTCGAACACGTCATGCGACTGC 4 559 1787 Antisense
CGACTGCTGCTCTCGATCAATTGAG 577 251 1805 Antisense
GAGCCAAAGTGACCTCGATGCTGCC 378 385 1920 Antisense
Sequence Source Affymetrix Proprietary Database