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[190666_s_at] cuticlin like

Description T24C2.1 /REP_DB=WormBase Gene ID /WP=CE27778 /GEN=ram-5 /TR=Q22724 /GB=CAA92199.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=cuticlin like
Cluster 6grp_total > 82      4grp_total > 49      6grp_cgc > 86      6grp_cgc_sub > 04      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 10.5
(-3)
A 		100.1
(3)
A 		82.1
(1.8)
A 		13
(-2.8)
A 		90
B 6.4
(-3)
A 		101.3
(2.3)
A 		113.3
(3)
A 		9.7
(-2.8)
A 		107
C 9.9
(-2.9)
A 		175.9
(3)
A 		150.6
(2.1)
A 		6.5
(-3)
A 		169
avr 8.9
(-3) 		125.8
(3) 		115.3
(2.5) 		9.7
(-3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 17.2
(-2.7)
A 		111.4
(3)
A 		11.8
(-3)
A 		100.6
(2.3)
A 		90.7
(1.8)
A 		73.8
(0.7)
A 		100
B 12.8
(-3)
A 		85.5
(2.2)
A 		97.2
(3)
A 		85.9
(2.2)
A 		83.9
(2.1)
A 		28.9
(-1.9)
A 		84
C 31.5
(-1.6)
A 		26
(-2.1)
A 		15
(-3)
A 		87.7
(3)
A 		77.8
(2.2)
A 		40
(-0.9)
A 		73
avr 20.5
(-3) 		74.3
(1.6) 		41.3
(-1.2) 		91.4
(3) 		84.1
(2.4) 		47.6
(-0.7) 		71
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 3.8
(-2.4)
A 		21.7
(3)
A 		21.5
(2.9)
A 		1.9
(-3)
A 		20
B 2.1
(-3)
A 		25
(2.7)
A 		26.3
(3)
A 		2.1
(-3)
A 		24
C 3.3
(-2.7)
A 		50.6
(2.8)
A 		52.5
(3)
A 		1.1
(-3)
A 		51
avr 3.1
(-2.7) 		32.4
(2.8) 		33.4
(3) 		1.7
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 3.2
(-2.9)
A 		39.6
(3)
A 		2.6
(-3)
A 		25.5
(0.7)
A 		27.4
(1)
A 		18
(-0.5)
A 		37
B 4.4
(-3)
A 		28.5
(1.9)
A 		23.6
(0.9)
A 		22.7
(0.7)
A 		34.1
(3)
A 		10.8
(-1.7)
A 		30
C 11.7
(-0.8)
A 		7.9
(-1.9)
A 		3.8
(-3)
A 		17.6
(0.9)
A 		25.2
(3)
A 		13.4
(-0.3)
A 		21
avr 6.4
(-3) 		25.3
(2) 		10
(-2) 		21.9
(1.1) 		28.9
(3) 		14.1
(-0.9) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190666_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17882
Sequence Type Exemplar sequence
Representative Public ID T24C2.1 NCBI
Target Description T24C2.1 /REP_DB=WormBase Gene ID /WP=CE27778 /GEN=ram-5 /TR=Q22724 /GB=CAA92199.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=cuticlin like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078021(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078021 NCBI Caenorhabditis elegans abnormal RAy Morphology family member (ram-5) (ram-5) mRNA, complete cds. 11/11 None
GENEFINDER00000015294 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14550068:14555969:1 9/11 None
SNAP00000015289 ENSEMBL cdna:SNAP chromosome:CEL140:X:14551654:14556110:1 11/11 None
T24C2.1 ENSEMBL cdna:known chromosome:CEL140:X:14550057:14556216:1 gene:T24C2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14550067-14556110(+) 100.0 100.0
Public Domain and Genome References
Gene Title cuticlin like
Gene Symbol ram-5
Ensembl T24C2.1
Entrez Gene 181550 Entrez gene
SwissProt Q22724 EMBL-EBI
WormBase CE31070 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92199 Hypothetical protein T24C2.1 [Caenorhabditis elegans] ref|NP_510422.2| abnormal RAy Morphology family member (ram-5) [Caenorhabditis elegans] gb|AAF67103.1| transmembrane protein RAM-5 [Caenorhabditis elegans] 0.0
blast CAE63196 Hypothetical protein CBG07551 [Caenorhabditis briggsae] 0.0
blast CAA91480 Hypothetical protein T04F8.4 [Caenorhabditis elegans] ref|NP_509946.1| T04F8.4 [Caenorhabditis elegans] 2.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 3.2E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510422.2 1 635-657
T24C2.1 1 635-657
GENEFINDER00000015294 1 635-657
SNAP00000015289 1 467-489
Sequence
Target Sequence 
>C. ELEGANS:190666_S_AT
tgccgccaacctgaagttctattcaacaacggcgtcgaccaaaaagttgaccccgaccac
tccgtatgcgtcggcgcccccggagagcagcactcaaactccaaactcgacgaagcccac
atccattagaaatgttactcatagtgtgccaacggttaaaaaatatgataaattcgtgaa
caataacgccgactggagatttgacgataaggcaatcaatgatagcgatatagtgtcgga
gaaacaaacttccgcatgctacaatgctacaattatttcatcacaacggcagtgtaaatg
gtcgggagtagaacatcttttactgatttggtctttcgcctcgttgatcgtttggatgat
gcttatagcattatttctctacagatactccagtaggaagccacaatggattggttttcg
gcaagtgccaatgcgaattcctgataattcgaaggtttttttttccagagagcaagaact
tcggagagtagctcaatctcgagtgttgagccaagatcatccatggcttcacgcagacgc
tttcgaagag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCGCCAACCTGAAGTTCTATTCA 563 583 1416 Antisense
AGTTCTATTCAACAACGGCGTCGAC 63 63 1430 Antisense
GAGAGCAGCACTCAAACTCCAAACT 372 395 1498 Antisense
GACGAAGCCCACATCCATTAGAAAT 358 379 1524 Antisense
GTTACTCATAGTGTGCCAACGGTTA 74 397 1549 Antisense
GAAACAAACTTCCGCATGCTACAAT 644 353 1656 Antisense
GCTACAATTATTTCATCACAACGGC 169 303 1681 Antisense
TTTACTGATTTGGTCTTTCGCCTCG 544 673 1734 Antisense
TTTCGCCTCGTTGATCGTTTGGATG 603 669 1749 Antisense
GCCAAGATCATCCATGGCTTCACGC 698 277 1925 Antisense
GCTTCACGCAGACGCTTTCGAAGAG 282 305 1941 Antisense
Sequence Source Affymetrix Proprietary Database