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[190664_s_at]

Description F27E11.3 /REP_DB=WormBase Gene ID /WP=CE09734 /CHR=5 /FEA=Sanger Annotation /DEF=transmembrane receptor (ST.LOUIS) TR:O16190 protein_id:AAB65257.1
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 17      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_highest_nonorm > 50      6grp_total > 17      4grp_total > 38      6grp_highest_sub > De      6grp_cgc > 12      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 492
1 2 3 4 range
A 694.8
(3)
P 		297.7
(-1.8)
P 		203.1
(-3)
P 		447.5
(-0)
P 		492
B 555
(3)
P 		398.8
(0.1)
P 		231.1
(-3)
P 		455.2
(1.2)
P 		324
C 686.5
(3)
P 		455
(-0.5)
P 		290.7
(-3)
P 		365.4
(-1.9)
P 		396
avr 645.4
(3) 		383.8
(-0.9) 		241.6
(-3) 		422.7
(-0.3) 		404

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 647
1 2 3 4 5 6 range
A 896.6
(3)
P 		512.2
(-0.6)
P 		313.8
(-2.4)
P 		249.3
(-3)
P 		356.5
(-2)
P 		354.1
(-2)
P 		647
B 816.5
(3)
P 		486.4
(-0.8)
P 		330.8
(-2.6)
P 		379.9
(-2)
P 		294.9
(-3)
P 		323.2
(-2.7)
P 		522
C 788.7
(3)
P 		469.7
(-0.6)
P 		335.3
(-2.1)
P 		253.1
(-3)
P 		346.9
(-1.9)
P 		357.9
(-1.8)
P 		536
avr 833.9
(3) 		489.4
(-0.8) 		326.6
(-2.6) 		294.1
(-3) 		332.8
(-2.6) 		345.1
(-2.4) 		540
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 200
1 2 3 4 range
A 253
(3)
P 		64.6
(-2.7)
P 		53.1
(-3)
P 		65.5
(-2.6)
P 		200
B 177
(3)
P 		98.5
(-0.8)
P 		53.5
(-3)
P 		97
(-0.9)
P 		124
C 227.3
(3)
P 		130.8
(-0.5)
P 		101.3
(-1.6)
P 		61.6
(-3)
P 		166
avr 219.1
(3) 		98
(-1.9) 		69.3
(-3) 		74.7
(-2.8) 		150

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 241
1 2 3 4 5 6 range
A 165.3
(2.1)
P 		182.2
(3)
P 		68.3
(-2.7)
P 		63.2
(-3)
P 		107.6
(-0.8)
P 		86.2
(-1.8)
P 		119
B 284.1
(3)
P 		161.9
(-0.6)
P 		80.3
(-3)
P 		100.6
(-2.4)
P 		119.8
(-1.8)
P 		120.5
(-1.8)
P 		204
C 292.3
(3)
P 		143.3
(-0.7)
P 		85.3
(-2.1)
P 		50.9
(-3)
P 		112.3
(-1.5)
P 		119.8
(-1.3)
P 		241
avr 247.2
(3) 		162.5
(0.1) 		78
(-2.8) 		71.6
(-3) 		113.2
(-1.6) 		108.8
(-1.7) 		176
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190664_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15452
Sequence Type Exemplar sequence
Representative Public ID F27E11.3 NCBI
Target Description F27E11.3 /REP_DB=WormBase Gene ID /WP=CE09734 /CHR=5 /FEA=Sanger Annotation /DEF=transmembrane receptor (ST.LOUIS) TR:O16190 protein_id:AAB65257.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071564(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071564 NCBI Caenorhabditis elegans Caenorhabditis FriZzled homolog family member (cfz-2) (cfz-2) mRNA, complete cds. 11/11 None
SNAP00000039153 ENSEMBL cdna:SNAP chromosome:CEL140:V:3441666:3444701:-1 11/11 None
GENEFINDER00000039156 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3441666:3444701:-1 11/11 None
F27E11.3a ENSEMBL cdna:known chromosome:CEL140:V:3441332:3444703:-1 gene:F27E11.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_071563 7/11 Cross Hyb Matching Probes None
F27E11.3b 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178768 2 1 NM_071564 173988_at (11), 190664_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3441665-3444584(-) 89.11 97.14
Public Domain and Genome References
Ensembl F27E11.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503965 Caenorhabditis FriZzled homolog family member (cfz-2) [Caenorhabditis elegans] 0.0
blast ABA54421 Caenorhabditis frizzled homolog protein 2, isoform a [Caenorhabditis elegans] gb|ABA18181.1| Frizzled homolog [Caenorhabditis elegans] 0.0
blast NP_503964 Caenorhabditis FriZzled homolog family member (cfz-2) [Caenorhabditis elegans] 0.0
blast ABA54422 Caenorhabditis frizzled homolog protein 2, isoform b [Caenorhabditis elegans] dbj|BAA84678.1| Cfz2 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000539 EMBL-EBI Frizzled protein 6.7E-108
Pfam IPR000024 EMBL-EBI Frizzled CRD region 3.2E-46
Pfam IPR000539 EMBL-EBI Frizzled protein 6.7E-108
Pfam IPR000024 EMBL-EBI Frizzled CRD region 3.3E-61
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503965.1 6 197-219,229-251,272-294,317-339,360-382,411-430
F27E11.3a 6 197-219,229-251,272-294,317-339,360-382,411-430
SNAP00000039153 6 236-258,268-290,311-333,356-378,399-421,450-469
GENEFINDER00000039156 6 236-258,268-290,311-333,356-378,399-421,450-469
Sequence
Target Sequence 
>C. ELEGANS:190664_S_AT
aaatcgggattttctcattgctctacacaattccatcgttattgattatctgtgttctat
tctacgagcagaatcatcgatcattatgggagcaatcgcagttgtgctcatgctcgccaa
agcaaactatcgaatttcatgataaaaattattcaaaaagtttaatttttccgcaaaatt
ttgcctcaaaaattgtgaatttctttccacatttccttttcaaaaattcgaacttccagc
taaaatttttctcagaaaaaaatttaaatttactgtcaaaaagtttctcagaaaatttga
atttccgccaaatttttttcctcaaaaaatgcgactcctcactgatcatctcccttatca
aaacttgctgtatgtgcattttgggatggacgtccggattttgggtgtgcagcaccaaaa
ctctatcgtcgtggaaaaatgctatttgctgtttgggttcctcccgatctttacccaaat
accaaccggcagacatactttacgctaaatctgacatgtcatcctcacaattctacaaca
caagtctccgacaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATCGGGATTTTCTCATTGCTCTA 620 113 1241 Antisense
TGCTCTACACAATTCCATCGTTATT 182 581 1259 Antisense
TTATCTGTGTTCTATTCTACGAGCA 267 679 1286 Antisense
TTGTGCTCATGCTCGCCAAAGCAAA 528 707 1342 Antisense
ATCATCTCCCTTATCAAAACTTGCT 690 29 1585 Antisense
GGACGTCCGGATTTTGGGTGTGCAG 164 525 1628 Antisense
TGCAGCACCAAAACTCTATCGTCGT 631 577 1648 Antisense
AATGCTATTTGCTGTTTGGGTTCCT 688 165 1678 Antisense
AACCGGCAGACATACTTTACGCTAA 455 145 1724 Antisense
GACATGTCATCCTCACAATTCTACA 32 371 1753 Antisense
TTCTACAACACAAGTCTCCGACACA 408 691 1771 Antisense
Sequence Source Affymetrix Proprietary Database