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[190661_s_at] Actin-binding protein /// Spectrin alpha chain

Description C07A9.5 /REP_DB=WormBase Gene ID /WP=CE02981 /TR=SW:P34316 /GB=CAA82343.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Actin-binding protein
Cluster 6grp_total > 79      4grp_total > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 0.9
(-3)
A 		2.6
(-2.3)
A 		15.9
(3)
A 		2.4
(-2.4)
A 		15
B 2.4
(-1.6)
A 		2.2
(-1.8)
A 		0.9
(-3)
A 		7.4
(3)
A 		7
C 7
(1)
A 		1.2
(-3)
A 		2.8
(-1.9)
A 		9.9
(3)
A 		9
avr 3.4
(-1.2) 		2
(-3) 		6.5
(2.9) 		6.6
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 42.6
(2.8)
A 		14.2
(-2.4)
A 		21.7
(-1)
A 		10.8
(-3)
A 		25.6
(-0.3)
A 		43.6
(3)
P 		33
B 13.2
(-1.4)
A 		0.8
(-3)
A 		2.6
(-2.8)
A 		3.1
(-2.7)
A 		16.6
(-1)
A 		47.1
(3)
P 		46
C 3.5
(-2.7)
A 		17.3
(-0.6)
A 		1.6
(-3)
A 		4.5
(-2.6)
A 		41.5
(3)
A 		39.9
(2.8)
A 		40
avr 19.8
(-0.8) 		10.8
(-2.2) 		8.6
(-2.6) 		6.1
(-3) 		27.9
(0.5) 		43.5
(3) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 0.3
(-3)
A 		0.6
(-2.5)
A 		4.2
(3)
A 		0.4
(-2.8)
A 		4
B 0.8
(-0.4)
A 		0.5
(-1.7)
A 		0.2
(-3)
A 		1.6
(3)
A 		1
C 2.3
(3)
A 		0.3
(-3)
A 		1
(-0.9)
A 		1.7
(1.2)
A 		2
avr 1.1
(-0.2) 		0.5
(-3) 		1.8
(3) 		1.2
(0.2) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 7.9
(0.9)
A 		5
(-1.3)
A 		4.7
(-1.5)
A 		2.7
(-3)
A 		7.7
(0.8)
A 		10.6
(3)
P 		8
B 4.6
(-1.5)
A 		0.3
(-3)
A 		0.6
(-2.9)
A 		0.8
(-2.8)
A 		6.7
(-0.8)
A 		17.6
(3)
P 		17
C 1.3
(-2.6)
A 		5.3
(-0.7)
A 		0.4
(-3)
A 		0.9
(-2.8)
A 		13.4
(3)
A 		13.4
(3)
A 		13
avr 4.6
(-1.5) 		3.5
(-2) 		1.9
(-2.8) 		1.5
(-3) 		9.3
(0.8) 		13.9
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190661_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6137
Sequence Type Exemplar sequence
Representative Public ID C07A9.5 NCBI
Target Description C07A9.5 /REP_DB=WormBase Gene ID /WP=CE02981 /TR=SW:P34316 /GB=CAA82343.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Actin-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066739(11), NM_066386(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066739 NCBI Caenorhabditis elegans C07A9.5 (C07A9.5) mRNA, complete cds. 11/11 A 
NM_066386 NCBI Caenorhabditis elegans C50C3.2 (C50C3.2) mRNA, complete cds. 11/11 A 
GENEFINDER00000039340 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9670798:9683204:-1 11/11 None
SNAP00000039324 ENSEMBL cdna:SNAP chromosome:CEL140:III:9681420:9682725:-1 11/11 None
SNAP00000020235 ENSEMBL cdna:SNAP chromosome:CEL140:III:8181854:8191066:1 11/11 None
GENEFINDER00000020242 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8181854:8193530:1 11/11 None
C50C3.2 ENSEMBL cdna:known chromosome:CEL140:III:8181854:8189292:1 gene:C50C3.2 11/11 A 
C07A9.5 ENSEMBL cdna:known chromosome:CEL140:III:9681420:9682725:-1 gene:C07A9.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9681415-9682721(-) 99.07 100.0
Public Domain and Genome References
Gene Title Actin-binding protein
Spectrin alpha chain
Ensembl C50C3.2
C07A9.5
Entrez Gene 176154 Entrez gene
182355 Entrez gene
SwissProt P34316 EMBL-EBI
P34367 EMBL-EBI
WormBase CE02981 Wormbase
CE01861 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA82343 Hypothetical protein C07A9.5 [Caenorhabditis elegans] ref|NP_499140.1| C07A9.5 [Caenorhabditis elegans] sp|P34316|YKT5_CAEEL Hypothetical protein C07A9.5 in chromosome III 0.0
blast AAA27974 Hypothetical protein C50C3.2 [Caenorhabditis elegans] ref|NP_498787.1| C50C3.2 [Caenorhabditis elegans] sp|P34367|YLJ2_CAEEL Hypothetical protein C50C3.2 in chromosome III 0.0
blast CAE70194 Hypothetical protein CBG16668 [Caenorhabditis briggsae] 0.0
blast S44622 C50C3.3 protein - Caenorhabditis elegans 0.0
blast CAA82344 Hypothetical protein C07A9.6 [Caenorhabditis elegans] ref|NP_001021158.1| C07A9.6 [Caenorhabditis elegans] sp|P34317|YKT6_CAEEL Hypothetical protein C07A9.6 in chromosome III precursor 0.0
blast CAE65154 Hypothetical protein CBG10022 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.0012
Pfam IPR002213 EMBL-EBI UDP-glucoronosyl/UDP-glucosyl transferase 6.2E-31
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.0012
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.0072
Pfam IPR002017 EMBL-EBI Spectrin repeat 0.053
Pfam IPR002017 EMBL-EBI Spectrin repeat 1.4E-4
Pfam IPR002017 EMBL-EBI Spectrin repeat 0.29
Pfam IPR002017 EMBL-EBI Spectrin repeat 8.5E-4
Pfam IPR002017 EMBL-EBI Spectrin repeat 0.13
Pfam IPR002017 EMBL-EBI Spectrin repeat 0.49
Pfam IPR002017 EMBL-EBI Spectrin repeat 6.4E-7
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.0072
Pfam IPR002017 EMBL-EBI Spectrin repeat 6.9E-4
Pfam IPR002017 EMBL-EBI Spectrin repeat 1.4E-4
Pfam IPR002017 EMBL-EBI Spectrin repeat 0.29
Pfam IPR002017 EMBL-EBI Spectrin repeat 8.5E-4
Pfam IPR002017 EMBL-EBI Spectrin repeat 0.13
Pfam IPR002017 EMBL-EBI Spectrin repeat 0.49
Pfam IPR002017 EMBL-EBI Spectrin repeat 6.4E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000039340 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:190661_S_AT
gtcttatggaatctcgttacatcttcgataacacattttcttctgccacaccacatggag
ttcttgtcgaaatgtgtcaatgtcttgagctgatgagttcaatgttgcgatctgtggagc
aatcaattgcagatagaaatcacaacggagtcactgagaaacagcttcacgagtttaaat
tagcctttgattatttcgatcaagaaaagaatggatggctagactatgagcacttcgagt
tgtgcctcaaatctcaaggatataacttttcagttgaaagtactctcaaagagacgatgt
ccctcttggatccatctacgtatgaaaagcatgactacatgcgttatatggtgaagcatg
agacaacaaacattcttgacgatcattcagccattgaagatgctttgaagaacctggatg
caaggaagatttcggatagtatgtctcgaaaagaagctgaattcttcatgagcaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTTATGGAATCTCGTTACATCTT 439 469 539 Antisense
TTCTGCCACACCACATGGAGTTCTT 636 693 579 Antisense
ACACCACATGGAGTTCTTGTCGAAA 630 109 586 Antisense
GTCAATGTCTTGAGCTGATGAGTTC 691 463 614 Antisense
TGCGATCTGTGGAGCAATCAATTGC 243 581 644 Antisense
GATAGAAATCACAACGGAGTCACTG 174 421 670 Antisense
GTCACTGAGAAACAGCTTCACGAGT 284 465 688 Antisense
GAAGCATGAGACAACAAACATTCTT 546 341 891 Antisense
CATTCTTGACGATCATTCAGCCATT 229 217 909 Antisense
AAGATGCTTTGAAGAACCTGGATGC 99 155 935 Antisense
GAAGCTGAATTCTTCATGAGCAAGA 576 343 991 Antisense
Sequence Source Affymetrix Proprietary Database