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[190657_at] pyruvate, water dikinase

Description T21C9.6 /REP_DB=WormBase Gene ID /WP=CE06475 /TR=Q22649 /GB=CAA97341.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=pyruvate, water dikinase
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 61      4grp_highest_8_8 > 43      4grp_high_8_8 > 48      6grp_highest_nonorm > 71      6grp_total > 51      4grp_total > 55      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 363
1 2 3 4 range
A 539.1
(3)
P 		175.9
(-3)
P 		329.5
(-0.5)
P 		187.4
(-2.8)
P 		363
B 498.2
(3)
P 		192.6
(-3)
P 		208.9
(-2.7)
P 		207.3
(-2.7)
P 		306
C 467.8
(3)
P 		224.4
(-3)
P 		328
(-0.4)
P 		246
(-2.5)
P 		243
avr 501.7
(3) 		197.6
(-3) 		288.8
(-1.2) 		213.6
(-2.7) 		304

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 239
1 2 3 4 5 6 range
A 212.7
(-3)
P 		350.6
(0.5)
P 		451.8
(3)
P 		448.1
(2.9)
P 		303.9
(-0.7)
P 		248.2
(-2.1)
P 		239
B 402.8
(1.3)
P 		324.4
(-1.8)
P 		403.6
(1.3)
P 		446.6
(3)
P 		293.3
(-3)
P 		297.9
(-2.8)
P 		153
C 321.6
(-0.5)
P 		413.1
(2.3)
P 		426.4
(2.6)
P 		438.4
(3)
P 		260.4
(-2.3)
P 		236
(-3)
P 		202
avr 312.4
(-1.3) 		362.7
(0.3) 		427.3
(2.4) 		444.4
(3) 		285.9
(-2.2) 		260.7
(-3) 		184
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 196.3
(3)
P 		38.2
(-2.6)
P 		86.1
(-0.9)
P 		27.4
(-3)
P 		169
B 158.9
(3)
P 		47.6
(-2.8)
P 		48.4
(-2.8)
P 		44.2
(-3)
P 		115
C 154.8
(3)
P 		64.5
(-1.8)
P 		114.3
(0.9)
P 		41.5
(-3)
P 		113
avr 170
(3) 		50.1
(-2.4) 		82.9
(-1) 		37.7
(-3) 		132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 39.2
(-3)
P 		124.7
(3)
P 		98.4
(1.2)
P 		113.6
(2.2)
P 		91.7
(0.7)
P 		60.4
(-1.5)
P 		86
B 140.1
(3)
P 		108
(-1.6)
P 		98
(-3)
P 		118.3
(-0.1)
P 		119.2
(0)
P 		111.1
(-1.1)
P 		42
C 119.2
(2.1)
P 		126.1
(3)
P 		108.5
(0.8)
P 		88.2
(-1.8)
P 		84.3
(-2.3)
P 		79
(-3)
P 		47
avr 99.5
(-0.3) 		119.6
(3) 		101.6
(0) 		106.7
(0.9) 		98.4
(-0.5) 		83.5
(-3) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190657_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11668
Sequence Type Exemplar sequence
Representative Public ID T21C9.6 NCBI
Target Description T21C9.6 /REP_DB=WormBase Gene ID /WP=CE06475 /TR=Q22649 /GB=CAA97341.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=pyruvate, water dikinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073314(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073314 NCBI Caenorhabditis elegans T21C9.6 (T21C9.6) mRNA, complete cds. 11/11 None
SNAP00000011986 ENSEMBL cdna:SNAP chromosome:CEL140:V:10584474:10589616:1 11/11 None
GENEFINDER00000011995 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10584474:10589616:1 11/11 None
T21C9.6 ENSEMBL cdna:known chromosome:CEL140:V:10584469:10589700:1 gene:T21C9.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10584473-10589616(+) 100.0 100.0
Public Domain and Genome References
Gene Title pyruvate, water dikinase
Ensembl T21C9.6
Entrez Gene 179478 Entrez gene
SwissProt Q22649 EMBL-EBI
WormBase CE06475 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
16310 phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
16301 kinase activity inferred from electronic annotation QuickGO AmiGO
16772 transferase activity, transferring phosphorus-containing groups inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA97341 Hypothetical protein T21C9.6 [Caenorhabditis elegans] ref|NP_505715.1| T21C9.6 [Caenorhabditis elegans] 0.0
blast CAE64852 Hypothetical protein CBG09648 [Caenorhabditis briggsae] 0.0
blast XP_790656 PREDICTED: similar to phosphoenolpyruvate synthase precursor (5L65) [Strongylocentrotus purpuratus] 2.0E-62
blast XP_790656 PREDICTED: similar to phosphoenolpyruvate synthase precursor (5L65) [Strongylocentrotus purpuratus] 3.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR002192 EMBL-EBI Pyruvate phosphate dikinase, PEP/pyruvate-binding 1.5E-114
Pfam IPR008279 EMBL-EBI PEP-utilising enzyme, mobile region 2.5E-30
Pfam IPR002192 EMBL-EBI Pyruvate phosphate dikinase, PEP/pyruvate-binding 1.3E-104
Pfam IPR008279 EMBL-EBI PEP-utilising enzyme, mobile region 2.5E-30
Pfam IPR002192 EMBL-EBI Pyruvate phosphate dikinase, PEP/pyruvate-binding 1.5E-114
Pfam IPR008279 EMBL-EBI PEP-utilising enzyme, mobile region 2.5E-30
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505715.1 1 5-27
T21C9.6 1 5-27
SNAP00000011986 1 5-27
GENEFINDER00000011995 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:190657_AT
atttagtttccacgacacattctcttcgaaaggcatgcagaatggttggaaagaagttgt
atgaaaaaggatatcttcctgatccaaatctttggatgcacttctcaatggacgaactta
aagaattgaatgcaacaagaagtgcaaaacttgtttccagagcaatcagaagaaaacaga
tttcatcgaagtttgaaggtctccaatttccacttgtttctcatggatacatgaatccaa
ttaaaatagaaatcgctgaaactgatgcaaatattggtatcattcttcgaggaactacag
tatgcgaaggaaaagttagagccagagcaagagttgccaaaacattggaagaagcaaaag
agacaaaaccaggagagattctcattacaaaatacacggatatctgttggtctccatttt
tcccaattatcagtggaattgtcaccgagattggaggactactcagtcatggggctgttg
ttgcaagagagtatggactaccaagtttgattgcagtttccaatgcaacatatcacttta
aaactggtgatcttgttgaactggactctatttctggcaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTAGTTTCCACGACACATTCTCT 453 15 3050 Antisense
GACACATTCTCTTCGAAAGGCATGC 393 367 3063 Antisense
GATATCTTCCTGATCCAAATCTTTG 214 427 3119 Antisense
AAATCTTTGGATGCACTTCTCAATG 689 117 3135 Antisense
TTTGAAGGTCTCCAATTTCCACTTG 250 665 3241 Antisense
TCCAATTTCCACTTGTTTCTCATGG 209 587 3251 Antisense
TACACGGATATCTGTTGGTCTCCAT 437 643 3442 Antisense
TGGTCTCCATTTTTCCCAATTATCA 112 551 3457 Antisense
GGACTACTCAGTCATGGGGCTGTTG 614 517 3505 Antisense
GCAGTTTCCAATGCAACATATCACT 530 311 3562 Antisense
TGAACTGGACTCTATTTCTGGCACA 611 573 3606 Antisense
Sequence Source Affymetrix Proprietary Database