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[190634_at]

Description F25F2.1 /REP_DB=WormBase Gene ID /WP=CE17702 /TR=Q19784 /GB=CAA84659.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 78      4grp_total > 12      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 11.1
(-2.1)
A 		13.9
(-1.6)
A 		6.3
(-3)
A 		38.9
(3)
A 		33
B 8
(-0.9)
A 		15.6
(3)
A 		3.9
(-3)
A 		11.9
(1.1)
A 		12
C 35.9
(-0.8)
A 		12.1
(-2.5)
A 		4.6
(-3)
A 		91.2
(3)
A 		87
avr 18.3
(-1.1) 		13.9
(-1.7) 		4.9
(-3) 		47.3
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 41.1
(-1)
A 		10.5
(-2.8)
A 		28.2
(-1.8)
A 		7.8
(-3)
A 		40.4
(-1)
A 		107.4
(3)
A 		100
B 24.5
(-3)
A 		31.1
(-2.5)
A 		26.2
(-2.9)
A 		39.6
(-1.9)
A 		50.6
(-1.1)
A 		106.8
(3)
A 		82
C 5.3
(-3)
A 		7.1
(-2.9)
A 		6.3
(-2.9)
A 		6
(-3)
A 		66
(0.2)
A 		118.8
(3)
A 		114
avr 23.6
(-2.5) 		16.2
(-3) 		20.2
(-2.7) 		17.8
(-2.9) 		52.3
(-0.7) 		111
(3) 		95
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 4.1
(0.7)
A 		3
(-1)
A 		1.6
(-3)
A 		5.7
(3)
A 		4
B 2.6
(0.4)
A 		3.9
(3)
A 		0.9
(-3)
A 		2.5
(0.2)
A 		3
C 11.9
(1.5)
A 		3.5
(-2.2)
A 		1.6
(-3)
A 		15.4
(3)
A 		14
avr 6.2
(1.4) 		3.5
(-1.1) 		1.4
(-3) 		7.9
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 7.6
(-1.6)
A 		3.7
(-2.6)
A 		6.1
(-2)
A 		2
(-3)
A 		12.2
(-0.5)
A 		26.2
(3)
A 		24
B 8.5
(-2.6)
A 		10.4
(-2.3)
A 		6.4
(-3)
A 		10.5
(-2.3)
A 		20.6
(-0.4)
A 		39.8
(3)
A 		33
C 2
(-2.9)
A 		2.2
(-2.8)
A 		1.6
(-2.9)
A 		1.2
(-3)
A 		21.4
(0.1)
A 		39.8
(3)
A 		39
avr 6
(-2.7) 		5.4
(-2.8) 		4.7
(-3) 		4.6
(-3) 		18.1
(-0.4) 		35.3
(3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190634_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7830
Sequence Type Exemplar sequence
Representative Public ID F25F2.1 NCBI
Target Description F25F2.1 /REP_DB=WormBase Gene ID /WP=CE17702 /TR=Q19784 /GB=CAA84659.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027379(10), NM_001027378(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027379 NCBI Caenorhabditis elegans F25F2.1b (F25F2.1) mRNA, complete cds. 10/11 None
NM_001027378 NCBI Caenorhabditis elegans F25F2.1a (F25F2.1) mRNA, complete cds. 11/11 None
SNAP00000027476 ENSEMBL cdna:SNAP chromosome:CEL140:III:4507409:4512092:1 10/11 None
GENEFINDER00000027483 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4509603:4512092:1 10/11 None
F25F2.1b ENSEMBL cdna:known chromosome:CEL140:III:4508827:4512092:1 gene:F25F2.1 10/11 None
F25F2.1a ENSEMBL cdna:known chromosome:CEL140:III:4509681:4512092:1 gene:F25F2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4509680-4512092(+) 100.0 100.0
Public Domain and Genome References
Ensembl F25F2.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD91629 Hypothetical protein F25F2.1b [Caenorhabditis elegans] ref|NP_001022550.1| F25F2.1b [Caenorhabditis elegans] 0.0
blast CAA84659 Hypothetical protein F25F2.1a [Caenorhabditis elegans] ref|NP_001022549.1| F25F2.1a [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.9E-6
Pfam IPR000719 EMBL-EBI Protein kinase 2.9E-6
Pfam IPR000719 EMBL-EBI Protein kinase 2.9E-6
Sequence
Target Sequence 
>C. ELEGANS:190634_AT
tctgctgaaacctctgccatggaaagacaacaacagaattgacaaagtggctcttgccaa
acgggattttatgtcacggctcccaaacaatctcacaaatcttccatcgcagattcttga
cattggacagatcttgaaaactgaatctccgtattcggaaatatcgaagaaacttgttga
aggtttggccagtgtcaaaaccagctcttcagaaaagtggaatccagaatggtacgatcg
ctcagctactagaaatcttcgagaaaagaaaatcaagaatcagccaagatctagagaatc
tgcagaagcagaagcaactgctgttcagacgacacctaccggaagtacggagttgcaaaa
tgatagaagagataagagtgtctacgtttcgactattgtaacaaaagttgtggataatcg
taagaaggtttcttcatcgaattcaccgacgactgttagtacgaataagacggctgtgat
agaagatgaggaagatgtcaagaagaacagtccaattcagaaacagcaagaccgttccgt
gtacttcaacgatttccctgttgctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTGCTGAAACCTCTGCCATGGAAA 548 607 789 Antisense
GTGGCTCTTGCCAAACGGGATTTTA 409 491 835 Antisense
TTTTATGTCACGGCTCCCAAACAAT 597 675 855 Antisense
CAATCTCACAAATCTTCCATCGCAG 530 181 876 Antisense
TTGGCCAGTGTCAAAACCAGCTCTT 271 705 973 Antisense
AGAATGGTACGATCGCTCAGCTACT 331 73 1014 Antisense
AGCAACTGCTGTTCAGACGACACCT 682 75 1101 Antisense
GAGTGTCTACGTTTCGACTATTGTA 165 401 1164 Antisense
GAATTCACCGACGACTGTTAGTACG 323 325 1227 Antisense
GAAACAGCAAGACCGTTCCGTGTAC 596 355 1308 Antisense
ACTTCAACGATTTCCCTGTTGCTGC 361 103 1331 Antisense
Sequence Source Affymetrix Proprietary Database