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[190628_at]

Description F32A5.4 /REP_DB=WormBase Gene ID /WP=CE01274 /TR=Q19948 /GB=AAC46663.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Immunodominant antigen
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 82      4grp_highest_8_8 > 39      4grp_high_8_8 > 20      6grp_highest_nonorm > 38      6grp_total > 74      4grp_total > 20      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3093
1 2 3 4 range
A 5005.7
(-0.8)
P 		4618
(-1.6)
P 		3986.4
(-3)
P 		6778.3
(3)
P 		2792
B 4803.9
(-1.1)
P 		4718.8
(-1.3)
P 		3950.8
(-3)
P 		6625.8
(3)
P 		2675
C 5085.1
(0.3)
P 		4590.4
(-0.9)
P 		3685.5
(-3)
P 		6246
(3)
P 		2561
avr 4964.9
(-0.6) 		4642.4
(-1.3) 		3874.2
(-3) 		6550
(3) 		2676

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3895
1 2 3 4 5 6 range
A 4874
(-3)
P 		5988.8
(-0.9)
P 		5968.5
(-0.9)
P 		8067.8
(3)
P 		6826.2
(0.7)
P 		7168.8
(1.3)
P 		3194
B 4533.8
(-3)
P 		5551.2
(-1.1)
P 		6954.6
(1.5)
P 		7788.9
(3)
P 		6943.1
(1.4)
P 		6025.3
(-0.3)
P 		3255
C 5194.1
(-3)
P 		5311.9
(-2.8)
P 		6306.7
(-0.9)
P 		8428.5
(3)
P 		7712.7
(1.7)
P 		6300
(-0.9)
P 		3234
avr 4867.3
(-3) 		5617.3
(-1.6) 		6409.9
(-0.1) 		8095.1
(3) 		7160.7
(1.3) 		6498
(0) 		3228
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 907
1 2 3 4 range
A 1822.6
(3)
P 		1002.4
(-2.9)
P 		1042.1
(-2.6)
P 		992.2
(-3)
P 		830
B 1531.9
(3)
P 		1165.4
(-0.6)
P 		915.5
(-3)
P 		1412
(1.8)
P 		616
C 1683.3
(3)
P 		1319.8
(-0.5)
P 		1284.5
(-0.8)
P 		1053.2
(-3)
P 		630
avr 1679.3
(3) 		1162.5
(-2.2) 		1080.7
(-3) 		1152.5
(-2.3) 		599

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1922
1 2 3 4 5 6 range
A 898.5
(-3)
P 		2129.9
(3)
P 		1299.8
(-1)
P 		2046.2
(2.6)
P 		2059.9
(2.7)
P 		1745.4
(1.1)
P 		1231
B 1577.5
(-3)
P 		1847.8
(-1.7)
P 		1688.8
(-2.5)
P 		2063
(-0.7)
P 		2820.9
(3)
P 		2246.8
(0.2)
P 		1243
C 1925.2
(-0.8)
P 		1620.8
(-2.9)
P 		1605.2
(-3)
P 		1695
(-2.4)
P 		2495.7
(3)
P 		2109
(0.4)
P 		891
avr 1467.1
(-3) 		1866.2
(-0.6) 		1531.3
(-2.6) 		1934.7
(-0.2) 		2458.8
(3) 		2033.7
(0.4) 		992
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190628_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4745
Sequence Type Exemplar sequence
Representative Public ID F32A5.4 NCBI
Target Description F32A5.4 /REP_DB=WormBase Gene ID /WP=CE01274 /TR=Q19948 /GB=AAC46663.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Immunodominant antigen
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063107(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063107 NCBI Caenorhabditis elegans F32A5.4a (F32A5.4) mRNA, complete cds. 11/11 None
SNAP00000015809 ENSEMBL cdna:SNAP chromosome:CEL140:II:7229084:7230383:1 11/11 None
GENEFINDER00000015822 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7229084:7230383:1 11/11 None
F32A5.4a.2 ENSEMBL cdna:known chromosome:CEL140:II:7228715:7230383:1 gene:F32A5.4 11/11 None
F32A5.4a.1 ENSEMBL cdna:known chromosome:CEL140:II:7229083:7230540:1 gene:F32A5.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182177 8/11 Cross Hyb Matching Probes None
F32A5.4b 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174188 2 1 NM_063107 190628_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7229079-7230379(+) 100.0 100.0
Public Domain and Genome References
Ensembl F32A5.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46663 Hypothetical protein F32A5.4a [Caenorhabditis elegans] ref|NP_495508.1| F32A5.4a [Caenorhabditis elegans] sp|Q19948|YPP4_CAEEL Hypothetical protein F32A5.4 in chromosome II precursor 1.0E-97
blast CAE67576 Hypothetical protein CBG13105 [Caenorhabditis briggsae] 3.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR010480 EMBL-EBI Pepsin inhibitor-3-likeed 1.2E-33
Pfam IPR010480 EMBL-EBI Pepsin inhibitor-3-likeed 3.7E-45
Sequence
Target Sequence 
>C. ELEGANS:190628_AT
cgactccgtaatttgacatcctctgagcagagcgagttggccacttaccaaactgaagtt
gagcaatacaagactcaactccgtaatattctcagccaacgtcgtgaaaacctccgtaac
cgtttgatgtctcaaggaagaaatcaacaacaacaaagcaatgatgtcagcagccaggga
ggaaatgacgatggatcaattccaaaggctccagaaaaaccatcattctgtactgctgaa
gatactactcaatactatttcgatggatgcatggttcaaggcaacaaagtctatgttggt
ggacaatacgctcgtgacctctcttctgatgagatttctgaattgcaaacattcgatact
caacaaaccgcttaccaaaatgccgttcaatcacagatgcaaagccaagttcaaggcctc
ttcggtggatctgacttcctttcagctttgttcgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACTCCGTAATTTGACATCCTCTG 11 253 166 Antisense
TGAGCAGAGCGAGTTGGCCACTTAC 545 569 189 Antisense
GACTCAACTCCGTAATATTCTCAGC 3 375 237 Antisense
CAGCCAACGTCGTGAAAACCTCCGT 146 1 258 Antisense
CCTCCGTAACCGTTTGATGTCTCAA 485 265 276 Antisense
AAACCATCATTCTGTACTGCTGAAG 392 125 382 Antisense
GTTGGTGGACAATACGCTCGTGACC 453 435 460 Antisense
ATTCGATACTCAACAAACCGCTTAC 397 9 516 Antisense
ACCGCTTACCAAAATGCCGTTCAAT 287 89 532 Antisense
CCAAGTTCAAGGCCTCTTCGGTGGA 165 271 570 Antisense
TGACTTCCTTTCAGCTTTGTTCGGA 158 571 597 Antisense
Sequence Source Affymetrix Proprietary Database