[Home] | [Total List]

[190624_s_at] leucyl-tRNA synthetase

Description R74.1 /REP_DB=WormBase Gene ID /WP=CE16317 /TR=SW:Q09996 /GB=CAA85280.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=leucyl-tRNA synthetase
Cluster 6grp_highest_10_10 > 54      6grp_high1_10_10 > 34      4grp_highest_8_8 > 37      4grp_high_8_8 > 01      6grp_highest_nonorm > 77      6grp_total > 01      4grp_total > 15      6grp_cgc > 26      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1293
1 2 3 4 range
A 1775.3
(3)
P 		768
(-3)
P 		946
(-1.9)
P 		1580.5
(1.8)
P 		1007
B 1621.7
(1.3)
P 		742.5
(-3)
P 		923.9
(-2.1)
P 		1981.4
(3)
P 		1239
C 1560.8
(1.6)
P 		688.4
(-3)
P 		1044.7
(-1.1)
P 		1838.3
(3)
P 		1150
avr 1652.6
(2.2) 		733
(-3) 		971.5
(-1.7) 		1800.1
(3) 		1067

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 628
1 2 3 4 5 6 range
A 1013.4
(-1.8)
P 		1317.2
(1.8)
P 		911.5
(-3)
P 		1423.2
(3)
P 		1068.7
(-1.2)
P 		1240.9
(0.9)
P 		512
B 1290
(-1.1)
P 		1539.8
(3)
P 		1207.1
(-2.4)
P 		1367.8
(0.2)
P 		1172.6
(-3)
P 		1298.2
(-0.9)
P 		367
C 1460.3
(3)
P 		1430.9
(2.4)
P 		1161.8
(-3)
P 		1260.1
(-1)
P 		1201.7
(-2.2)
P 		1249.8
(-1.2)
P 		299
avr 1254.6
(-0.1) 		1429.3
(3) 		1093.5
(-3) 		1350.4
(1.6) 		1147.7
(-2) 		1263
(0) 		336
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 480
1 2 3 4 range
A 646.4
(3)
P 		166.7
(-3)
P 		247.3
(-2)
P 		231.3
(-2.2)
P 		480
B 517.1
(3)
P 		183.4
(-3)
P 		214.1
(-2.4)
P 		422.2
(1.3)
P 		334
C 516.7
(3)
P 		197.9
(-3)
P 		364.1
(0.1)
P 		310
(-0.9)
P 		319
avr 560.1
(3) 		182.7
(-3) 		275.2
(-1.5) 		321.2
(-0.8) 		377

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 355
1 2 3 4 5 6 range
A 186.8
(-3)
P 		468.5
(3)
P 		198.5
(-2.8)
P 		361
(0.7)
P 		322.5
(-0.1)
P 		302.1
(-0.5)
P 		282
B 448.9
(1.3)
P 		512.5
(3)
P 		293.1
(-3)
P 		362.3
(-1.1)
P 		476.4
(2)
P 		484.1
(2.2)
P 		219
C 541.3
(3)
P 		436.6
(0.8)
P 		295.7
(-2.1)
P 		253.4
(-3)
P 		388.8
(-0.2)
P 		418.4
(0.4)
P 		288
avr 392.3
(0.7) 		472.5
(3) 		262.4
(-3) 		325.6
(-1.2) 		395.9
(0.8) 		401.5
(1) 		210
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190624_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6726
Sequence Type Exemplar sequence
Representative Public ID R74.1 NCBI
Target Description R74.1 /REP_DB=WormBase Gene ID /WP=CE16317 /TR=SW:Q09996 /GB=CAA85280.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=leucyl-tRNA synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065436(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065436 NCBI Caenorhabditis elegans Leucyl tRNA Synthetase family member (lrs-1) (lrs-1) mRNA, complete cds. 11/11 None
R74.1.3 ENSEMBL cdna:known chromosome:CEL140:III:4181060:4186755:-1 gene:R74.1 11/11 None
R74.1.1 ENSEMBL cdna:known chromosome:CEL140:III:4181524:4186794:-1 gene:R74.1 11/11 None
R74.1.2 ENSEMBL cdna:known chromosome:CEL140:III:4181525:4186772:-1 gene:R74.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4181682-4186755(-) 100.0 100.0
Public Domain and Genome References
Gene Title leucyl-tRNA synthetase
Gene Symbol lrs-1
Ensembl R74.1
Entrez Gene 175538 Entrez gene
SwissProt Q09996 EMBL-EBI
WormBase CE16317 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264705_AT tRNA synthetase class I (I, L, M and V) family protein at
ATGENOME1:18760_AT tRNA synthetase class I (I, L, M and V) family protein at
CANINE:1587430_AT similar to Leucyl-tRNA synthetase, cytoplasmic (Leucine--tRNA ligase) (LeuRS) cfa
CANINE_2:CFA.1743.1.A1_AT similar to Leucyl-tRNA synthetase, cytoplasmic (Leucine--tRNA ligase) (LeuRS) cfa
CANINE_2:CFAAFFX.10630.1.S1_AT similar to Leucyl-tRNA synthetase, cytoplasmic (Leucine--tRNA ligase) (LeuRS) cfa
CANINE_2:CFAAFFX.10630.1.S1_S_AT similar to Leucyl-tRNA synthetase, cytoplasmic (Leucine--tRNA ligase) (LeuRS) cfa
DROSGENOME1:151752_S_AT dm
DROSGENOME1:151753_R_AT dm
DROSOPHILA_2:1623193_AT dm
CHICKEN:GGA.18531.1.S1_S_AT leucyl-tRNA synthetase gga
CHICKEN:GGA.7312.1.S1_AT leucyl-tRNA synthetase gga
CHICKEN:GGAAFFX.22261.1.S1_S_AT leucyl-tRNA synthetase gga
CHICKEN:GGAAFFX.4666.1.S1_AT leucyl-tRNA synthetase gga
CHICKEN:GGAAFFX.4666.1.S1_S_AT leucyl-tRNA synthetase gga
HG-U133_PLUS_2:222428_S_AT leucyl-tRNA synthetase hs
HG-U133B:222428_S_AT leucyl-tRNA synthetase hs
HG-U133_PLUS_2:223888_S_AT leucyl-tRNA synthetase hs
HG-U133B:223888_S_AT leucyl-tRNA synthetase hs
HG-U133_PLUS_2:217810_X_AT leucyl-tRNA synthetase hs
HG-U133A:217810_X_AT leucyl-tRNA synthetase hs
HG-U133A_2:217810_X_AT leucyl-tRNA synthetase hs
HU35KSUBA:R32993_S_AT leucyl-tRNA synthetase hs
HU35KSUBA:RC_AA609797_AT leucyl-tRNA synthetase hs
HU35KSUBC:RC_AA599443_F_AT leucyl-tRNA synthetase hs
HU35KSUBC:RC_AA599443_R_AT leucyl-tRNA synthetase hs
U133_X3P:G7804449_3P_A_AT leucyl-tRNA synthetase hs
U133_X3P:G7106773_3P_S_AT leucyl-tRNA synthetase hs
U133_X3P:HS.6762.0.A1_3P_A_AT leucyl-tRNA synthetase hs
HG-U133_PLUS_2:222427_S_AT leucyl-tRNA synthetase hs
HG-U133B:222427_S_AT leucyl-tRNA synthetase hs
HG-U95B:54061_AT leucyl-tRNA synthetase hs
HG-U95C:61729_R_AT leucyl-tRNA synthetase hs
HG-U133_PLUS_2:243147_X_AT Leucyl-tRNA synthetase hs
HG-U133B:243147_X_AT Leucyl-tRNA synthetase hs
HG-U95C:66220_R_AT Leucyl-tRNA synthetase hs
MU11KSUBB:MSA.5543.0_S_AT leucyl-tRNA synthetase mm
MG-U74AV2:103630_AT leucyl-tRNA synthetase mm
MOE430A:1448403_AT leucyl-tRNA synthetase mm
MOUSE430A_2:1448403_AT leucyl-tRNA synthetase mm
MOUSE430_2:1448403_AT leucyl-tRNA synthetase mm
MG-U74CV2:130137_AT Leucyl-tRNA synthetase, mRNA (cDNA clone MGC:8232 IMAGE:3591521) mm
MU19KSUBB:TC29830_AT Leucyl-tRNA synthetase, mRNA (cDNA clone MGC:8232 IMAGE:3591521) mm
MU19KSUBC:TC39347_AT Leucyl-tRNA synthetase, mRNA (cDNA clone MGC:8232 IMAGE:3591521) mm
RG-U34B:RC_AA944176_AT leucyl-tRNA synthetase (predicted) rn
RAE230A:1374118_AT leucyl-tRNA synthetase (predicted) rn
RAT230_2:1374118_AT leucyl-tRNA synthetase (predicted) rn
RAE230B:1395018_AT leucyl-tRNA synthetase (predicted) rn
RAT230_2:1395018_AT leucyl-tRNA synthetase (predicted) rn
YEAST_2:1776027_AT Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
4823 leucine-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86751 Hypothetical protein R74.1 [Caenorhabditis elegans] emb|CAA85280.1| Hypothetical protein R74.1 [Caenorhabditis elegans] ref|NP_497837.1| Leucyl tRNA Synthetase family member (lrs-1) [Caenorhabditis elegans] sp|Q09996|SYLC_CAEEL Leucyl-tRNA synthetase (Leucine--tRNA ligase) (LeuRS) 0.0
blast CAE72860 Hypothetical protein CBG20159 [Caenorhabditis briggsae] 0.0
blast NP_064502 leucyl-tRNA synthetase [Homo sapiens] dbj|BAA95667.1| leucyl tRNA synthetase [Homo sapiens] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002300 EMBL-EBI Aminoacyl-tRNA synthetase, class Ia 1.8E-14
Sequence
Target Sequence 
>C. ELEGANS:190624_S_AT
agggtgtttcagcattggcttcttctagtccagttgatcaaacttcaattctcaacgaga
atattgattttattatgaacgctttggatttggatcgtgttactattcgacatactgatg
aggagggaattgatgcgaatattgttgagactacagtaccgctggtgccaatgttgaatt
tcacgccgaatagaccaacaatcaaacttgtcgccagaaatgttcaaatctgcaatgcaa
tgttcgatgttgatgtaccaattgttaatggagacagtgtttcaatggttattcggaaga
tgagacgcatcagcaaggcaatcaaaccgaaattcgaagtgagcctctggagatacaaga
acgcggtttggggagatcgacagatgatttcgtacagaaatccattcgaggagaacattc
aattgtccgacgcagacattttcaatttcgaggctgataacaagatttctgtgacttctg
gaagtgaaaagttcgatcttgggcgtactatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGGTGTTTCAGCATTGGCTTCTTC 513 57 3032 Antisense
GCTTCTTCTAGTCCAGTTGATCAAA 4 307 3049 Antisense
GATCAAACTTCAATTCTCAACGAGA 494 421 3067 Antisense
GGATCGTGTTACTATTCGACATACT 133 513 3123 Antisense
TGAGACTACAGTACCGCTGGTGCCA 86 569 3177 Antisense
TGGTGCCAATGTTGAATTTCACGCC 328 553 3194 Antisense
CGAAATTCGAAGTGAGCCTCTGGAG 99 253 3359 Antisense
GAACGCGGTTTGGGGAGATCGACAG 425 347 3390 Antisense
GAACATTCAATTGTCCGACGCAGAC 195 351 3444 Antisense
GACGCAGACATTTTCAATTTCGAGG 247 381 3460 Antisense
AAAGTTCGATCTTGGGCGTACTATT 217 123 3519 Antisense
Sequence Source Affymetrix Proprietary Database