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[190622_at] serine/threonine kinase

Description ZK666.8 /REP_DB=WormBase Gene ID /WP=CE02393 /TR=Q23566 /GB=CAA88987.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 79      4grp_total > 10      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 45.6
(-2.8)
A 		44.9
(-3)
A 		62.9
(0.9)
A 		72.5
(3)
A 		28
B 8.7
(-3)
A 		54
(3)
A 		24
(-1)
A 		41
(1.3)
A 		45
C 49.7
(0.3)
A 		13.7
(-3)
A 		19.2
(-2.5)
A 		80
(3)
A 		66
avr 34.7
(-3) 		37.5
(-2.4) 		35.4
(-2.9) 		64.5
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 75.3
(2.4)
A 		39.1
(-3)
A 		46
(-2)
A 		44
(-2.3)
A 		77.1
(2.7)
A 		79
(3)
A 		40
B 9.1
(-3)
A 		67.8
(1.2)
A 		31.1
(-1.4)
A 		62.7
(0.8)
A 		49.9
(-0.1)
A 		93.1
(3)
A 		84
C 35.7
(-0.1)
A 		46.9
(1.3)
A 		35.1
(-0.2)
A 		11.4
(-3)
A 		30.1
(-0.8)
A 		61.5
(3)
M 		50
avr 40
(-2.6) 		51.3
(-0.9) 		37.4
(-3) 		39.4
(-2.7) 		52.4
(-0.8) 		77.9
(3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 16.6
(3)
A 		9.7
(-3)
A 		16.5
(2.9)
A 		10.6
(-2.2)
A 		7
B 2.8
(-3)
A 		13.3
(3)
A 		5.6
(-1.4)
A 		8.7
(0.4)
A 		11
C 16.5
(3)
A 		3.9
(-3)
A 		6.7
(-1.7)
A 		13.5
(1.6)
A 		13
avr 12
(3) 		9
(-3) 		9.6
(-1.8) 		10.9
(0.8) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 13.9
(-1.2)
A 		13.9
(-1.2)
A 		10
(-3)
A 		11.2
(-2.5)
A 		23.3
(3)
A 		19.2
(1.2)
A 		13
B 3.2
(-3)
A 		22.6
(0.7)
A 		7.5
(-2.2)
A 		16.6
(-0.4)
A 		20.3
(0.3)
A 		34.7
(3)
A 		32
C 13.2
(0.6)
A 		14.3
(0.9)
A 		8.9
(-0.8)
A 		2.3
(-3)
A 		9.7
(-0.6)
A 		20.6
(3)
M 		18
avr 10.1
(-2.5) 		16.9
(0) 		8.8
(-3) 		10
(-2.6) 		17.8
(0.4) 		24.8
(3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190622_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2632
Sequence Type Exemplar sequence
Representative Public ID ZK666.8 NCBI
Target Description ZK666.8 /REP_DB=WormBase Gene ID /WP=CE02393 /TR=Q23566 /GB=CAA88987.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063860(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063860 NCBI Caenorhabditis elegans ZK666.8 (ZK666.8) mRNA, complete cds. 11/11 None
SNAP00000005239 ENSEMBL cdna:SNAP chromosome:CEL140:II:10491629:10492872:-1 11/11 None
GENEFINDER00000005244 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10491629:10492992:-1 11/11 None
ZK666.8 ENSEMBL cdna:known chromosome:CEL140:II:10491577:10492872:-1 gene:ZK666.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10491625-10492869(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase
Ensembl ZK666.8
Entrez Gene 191385 Entrez gene
SwissProt Q23566 EMBL-EBI
WormBase CE02393 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88987 Hypothetical protein ZK666.8 [Caenorhabditis elegans] ref|NP_496261.1| ZK666.8 [Caenorhabditis elegans] 0.0
blast CAE67455 Hypothetical protein CBG12956 [Caenorhabditis briggsae] 1.0E-140
blast AAC24425 Hypothetical protein T19D12.5 [Caenorhabditis elegans] ref|NP_495349.1| T19D12.5 [Caenorhabditis elegans] 1.0E-94
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-19
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-19
Sequence
Target Sequence 
>C. ELEGANS:190622_AT
aagaatcgccgttcaggccattgctgctctgcgagatctacacgacgtcgggtatctcca
tagagatattaagccaggaaatatgtgttttggtatcacttccaaaacacgtcatgtttt
gatgcttctcgactttggtctggtacgtagatacaaagatccagatggtgaatggcgaac
tcatcgggttaaagctggtttccgaggcactcaaagatatgtatctactcgtgttcatcg
tcggctcgaacagacaccgacagacgacatggtgtccttgttgtacacattgattgagtt
gctggctggagagcttccgtggagaaatattgaaaacagtgatgccatctggaaaatgaa
agatgaattgcatcatggccaaattgatcacttccatgaatcgtctcaagagctgattga
attttctcaactcgtatcacgtctcgacccaatgtctgaacttccgtatactactcttca
agcatccgtcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAATCGCCGTTCAGGCCATTGCT 194 151 519 Antisense
CATTGCTGCTCTGCGAGATCTACAC 377 217 537 Antisense
GATCTACACGACGTCGGGTATCTCC 218 419 553 Antisense
GCTTCTCGACTTTGGTCTGGTACGT 341 305 642 Antisense
TAAAGCTGGTTTCCGAGGCACTCAA 186 641 708 Antisense
TTCATCGTCGGCTCGAACAGACACC 16 689 752 Antisense
GATCACTTCCATGAATCGTCTCAAG 463 421 904 Antisense
GAATTTTCTCAACTCGTATCACGTC 438 325 937 Antisense
CACGTCTCGACCCAATGTCTGAACT 463 199 956 Antisense
TGTCTGAACTTCCGTATACTACTCT 663 555 971 Antisense
ATACTACTCTTCAAGCATCCGTCAA 255 23 986 Antisense
Sequence Source Affymetrix Proprietary Database