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[190613_at]

Description Y52D3.1 /REP_DB=WormBase Gene ID /WP=CE19223 /TR=Q9XTC8 /GB=CAB16482.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 78      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 82      6grp_total > 69      4grp_total > 13      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 283
1 2 3 4 range
A 381.9
(2.7)
P 		219.3
(-1.8)
P 		178
(-3)
P 		393
(3)
P 		215
B 251.5
(-1)
P 		226.5
(-1.5)
P 		155.5
(-3)
P 		438.2
(3)
P 		283
C 334.4
(1.8)
P 		194.3
(-3)
P 		222.8
(-2)
P 		368.6
(3)
P 		174
avr 322.6
(0.8) 		213.4
(-2.2) 		185.4
(-3) 		399.9
(3) 		215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 332
1 2 3 4 5 6 range
A 349.8
(-0.9)
P 		282.6
(-3)
P 		307
(-2.2)
P 		339.6
(-1.2)
P 		300.6
(-2.4)
P 		476.1
(3)
P 		194
B 331.8
(-0)
P 		189
(-3)
P 		216.1
(-2.4)
P 		287.3
(-1)
P 		287.9
(-0.9)
P 		476.8
(3)
P 		288
C 364.7
(-0.8)
P 		276.1
(-3)
P 		317.6
(-2)
P 		311.6
(-2.1)
P 		376.5
(-0.5)
P 		520.9
(3)
P 		245
avr 348.8
(-0.5) 		249.2
(-3) 		280.2
(-2.2) 		312.8
(-1.4) 		321.7
(-1.2) 		491.3
(3) 		242
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 139.1
(3)
P 		47.6
(-2.9)
P 		46.5
(-3)
P 		57.5
(-2.3)
P 		93
B 80.2
(1.6)
P 		56
(-0.9)
P 		36
(-3)
P 		93.4
(3)
P 		57
C 110.7
(3)
P 		55.9
(-3)
P 		77.7
(-0.6)
P 		62.2
(-2.3)
P 		55
avr 110
(3) 		53.2
(-3) 		53.4
(-3) 		71
(-1.1) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 64.5
(-3)
P 		100.5
(1.2)
P 		66.9
(-2.7)
P 		86.1
(-0.5)
P 		90.7
(0.1)
P 		115.9
(3)
P 		51
B 115.4
(0)
P 		62.9
(-2.5)
P 		52.5
(-3)
P 		76.1
(-1.9)
P 		117
(0.1)
P 		177.8
(3)
P 		125
C 135.2
(0.9)
P 		84.3
(-1.8)
P 		80.8
(-2)
P 		62.7
(-3)
P 		121.8
(0.2)
P 		174.4
(3)
P 		112
avr 105
(-0.4) 		82.6
(-1.9) 		66.7
(-3) 		75
(-2.4) 		109.8
(-0.1) 		156
(3) 		89
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190613_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7325
Sequence Type Exemplar sequence
Representative Public ID Y52D3.1 NCBI
Target Description Y52D3.1 /REP_DB=WormBase Gene ID /WP=CE19223 /TR=Q9XTC8 /GB=CAB16482.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NR_002379(11), NM_001027731(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NR_002379 NCBI Caenorhabditis elegans Y52D3.1, miscRNA. 11/11 None
NM_001027731 NCBI Caenorhabditis elegans Y52D3.1a (Y52D3.1) mRNA, complete cds. 11/11 None
SNAP00000024532 ENSEMBL cdna:SNAP chromosome:CEL140:III:10947966:10950861:1 11/11 None
GENEFINDER00000024534 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10949113:10950861:1 11/11 None
Y52D3.1a.3 ENSEMBL cdna:known chromosome:CEL140:III:10947322:10950861:1 gene:Y52D3.1 11/11 None
Y52D3.1a.1 ENSEMBL cdna:known chromosome:CEL140:III:10947323:10951046:1 gene:Y52D3.1 11/11 None
Y52D3.1a.2 ENSEMBL cdna:known chromosome:CEL140:III:10947332:10951046:1 gene:Y52D3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10947331-10950857(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y52D3.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB16482 Hypothetical protein Y52D3.1a [Caenorhabditis elegans] emb|CAA19523.1| Hypothetical protein Y52D3.1a [Caenorhabditis elegans] ref|NP_001022902.1| Y52D3.1a [Caenorhabditis elegans] 0.0
blast CAE71368 Hypothetical protein CBG18272 [Caenorhabditis briggsae] 1.0E-151
blast CAE71368 Hypothetical protein CBG18272 [Caenorhabditis briggsae] 1.0E-138
Protein Families
Method ID Description E-Value
hanks 2.1.13 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; ACMPK 6.0E-19
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.8E-30
Pfam IPR000719 EMBL-EBI Protein kinase 9.6E-16
Pfam IPR000719 EMBL-EBI Protein kinase 0.0018
Pfam IPR000719 EMBL-EBI Protein kinase 2.0E-14
Pfam IPR000719 EMBL-EBI Protein kinase 3.1E-31
Pfam IPR000719 EMBL-EBI Protein kinase 9.6E-16
Sequence
Target Sequence 
>C. ELEGANS:190613_AT
cgactccagcggcaacgtgaagctctcgggattccgattcatgatcgaactgaatcatca
cctggattgtgtgttcgaatttgatgctcatctccaaaatcagctctactatttggcacc
ggaagtgctcgcacagaatatacacggatacacgtcgaaatccgacatctacatgttggg
catatcgatttgtgaggcaatcaatggagttatgccttttggagagcttgaaccacttga
aatgctccatcggaagcttaatggacaggttccgagaccggtggacatgattagtttgaa
ggatgatcagaagatgggattggacatttcccaccgcccccaggagcacttgacacgtcg
attttcgaaagaaatgcacgaattcatcgccaactgcctcgactatgatccacaacagag
aggctctgcgtccgacctgaaatcgtccgcgtggctcgggtcaaaaattcacaaaaatct
gggcccggtagatgtccgtcaagagcttaacctggactatgcacatttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACTCCAGCGGCAACGTGAAGCTC 548 251 561 Antisense
GAAGCTCTCGGGATTCCGATTCATG 554 341 579 Antisense
AAATCAGCTCTACTATTTGGCACCG 382 117 657 Antisense
TTGGCACCGGAAGTGCTCGCACAGA 464 709 673 Antisense
TGAACCACTTGAAATGCTCCATCGG 150 575 789 Antisense
GGACAGGTTCCGAGACCGGTGGACA 506 525 823 Antisense
GGAGCACTTGACACGTCGATTTTCG 545 519 903 Antisense
AATGCACGAATTCATCGCCAACTGC 618 165 933 Antisense
TGCCTCGACTATGATCCACAACAGA 547 585 955 Antisense
GGCCCGGTAGATGTCCGTCAAGAGC 696 471 1042 Antisense
GCTTAACCTGGACTATGCACATTTC 399 305 1065 Antisense
Sequence Source Affymetrix Proprietary Database