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[190612_at] Yeast SOH1 protein like

Description F32H2.2 /REP_DB=WormBase Gene ID /WP=CE09877 /TR=P91869 /GB=CAB04242.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Yeast SOH1 protein like
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 84      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 63      6grp_total > 74      4grp_total > 02      6grp_cgc > 66      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1220
1 2 3 4 range
A 497.4
(-2.8)
P 		509.9
(-2.8)
P 		467
(-3)
P 		1556.5
(3)
P 		1090
B 529.2
(-1.9)
P 		365.4
(-3)
P 		545.1
(-1.8)
P 		1280.7
(3)
P 		915
C 477.5
(-3)
P 		509.7
(-2.8)
P 		508.9
(-2.8)
P 		1585.1
(3)
P 		1108
avr 501.4
(-2.8) 		461.7
(-3) 		507
(-2.7) 		1474.1
(3) 		1012

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 525
1 2 3 4 5 6 range
A 373.4
(-3)
P 		412.9
(-2.5)
P 		898.5
(3)
P 		775.8
(1.6)
P 		615.8
(-0.2)
P 		594.4
(-0.5)
P 		525
B 469.1
(-3)
P 		590.9
(-1.2)
P 		761.6
(1.3)
P 		570.8
(-1.5)
P 		779.3
(1.6)
P 		873
(3)
P 		404
C 450.6
(-3)
P 		577.1
(-1.1)
P 		596.8
(-0.8)
P 		676.6
(0.3)
P 		687.3
(0.5)
P 		858.1
(3)
P 		408
avr 431
(-3) 		527
(-1.3) 		752.3
(2.6) 		674.4
(1.2) 		694.1
(1.6) 		775.2
(3) 		344
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 181.1
(0.6)
P 		110.7
(-3)
P 		122.1
(-2.4)
P 		227.8
(3)
P 		117
B 168.8
(-0.4)
P 		90.2
(-3)
P 		126.3
(-1.8)
P 		272.9
(3)
P 		183
C 158.1
(-2.4)
P 		146.5
(-3)
P 		177.4
(-1.5)
P 		267.3
(3)
P 		121
avr 169.3
(-0.7) 		115.8
(-3) 		141.9
(-1.9) 		256
(3) 		140

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 257
1 2 3 4 5 6 range
A 68.8
(-3)
P 		146.8
(0.7)
P 		195.7
(2.9)
P 		196.8
(3)
P 		185.8
(2.5)
P 		144.7
(0.6)
P 		128
B 163.2
(-2.6)
P 		196.7
(-1.4)
P 		185
(-1.8)
P 		151.2
(-3)
P 		316.6
(2.7)
P 		325.6
(3)
P 		174
C 167
(-1.8)
P 		176.1
(-1.4)
P 		151.9
(-2.4)
P 		136.1
(-3)
P 		222.4
(0.4)
P 		287.2
(3)
P 		151
avr 133
(-3) 		173.2
(-1) 		177.5
(-0.8) 		161.4
(-1.6) 		241.6
(2.5) 		252.5
(3) 		120
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190612_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2213
Sequence Type Exemplar sequence
Representative Public ID F32H2.2 NCBI
Target Description F32H2.2 /REP_DB=WormBase Gene ID /WP=CE09877 /TR=P91869 /GB=CAB04242.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Yeast SOH1 protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060012(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060012 NCBI Caenorhabditis elegans MeDiaTor family member (mdt-31) (mdt-31) mRNA, complete cds. 11/11 None
GENEFINDER00000013780 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8974254:8975086:1 11/11 None
SNAP00000013728 ENSEMBL cdna:SNAP chromosome:CEL140:I:8974432:8975086:1 11/11 None
F32H2.2 ENSEMBL cdna:known chromosome:CEL140:I:8974438:8975086:1 gene:F32H2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8974368-8975017(+) 100.0 100.0
Public Domain and Genome References
Gene Title Yeast SOH1 protein like
Gene Symbol mdt-31
Ensembl F32H2.2
Entrez Gene 185213 Entrez gene
SwissProt P91869 EMBL-EBI
WormBase CE09877 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246140_AT SOH1 family protein at
DROSOPHILA_2:1641160_S_AT Mediator complex subunit 31 dm
DROSGENOME1:154738_AT Mediator complex subunit 31 dm
CHICKEN:GGA.12072.1.S1_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) gga
CHICKEN:GGA.12072.2.S1_A_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) gga
CHICKEN:GGA.19938.1.A1_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) gga
HG-U133A:219318_X_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U133_PLUS_2:219318_X_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U133A_2:219318_X_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U133_PLUS_2:222867_S_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U133B:222867_S_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HU35KSUBD:RC_AA442887_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
U133_X3P:G7705591_3P_A_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
U133_X3P:HS.27289.0.S1_3P_A_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U95B:58511_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U133_PLUS_2:236241_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U133B:236241_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
U133_X3P:HS.145037.0.A1_3P_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U95E:82706_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U133_PLUS_2:243319_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U133B:243319_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U95B:46420_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HG-U95B:58729_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
HU35KSUBC:RC_AA411743_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
U133_X3P:HS.43566.0.A1_3P_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) hs
MOUSE430A_2:1417387_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) mm
MOE430A:1417387_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) mm
MOUSE430_2:1417387_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) mm
MG-U74BV2:162622_AT mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) mm
MU19KSUBA:TC15863_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast), mRNA (cDNA clone MGC:30726 IMAGE:3983617) mm
MU19KSUBA:TC21296_AT Mediator of RNA polymerase II transcription, subunit 31 homolog (yeast), mRNA (cDNA clone MGC:30726 IMAGE:3983617) mm
RAE230B:1393276_AT similar to CGI-125 protein (predicted) rn
RAT230_2:1393276_AT similar to CGI-125 protein (predicted) rn
RAE230B:1392659_AT Similar to CGI-125 protein (predicted) rn
RAT230_2:1392659_AT Similar to CGI-125 protein (predicted) rn
RG-U34B:RC_AI045496_AT Similar to CGI-125 protein (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
45449 regulation of transcription inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
119 mediator complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16455 RNA polymerase II transcription mediator activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04242 Hypothetical protein F32H2.2 [Caenorhabditis elegans] ref|NP_492413.1| MeDiaTor family member (mdt-31) [Caenorhabditis elegans] sp|P91869|MED31_CAEEL Probable mediator of RNA polymerase II transcription subunit 31 (Mediator complex subunit 31) (Mediator complex subunit soh-1) 3.0E-75
blast CAB04242 Hypothetical protein F32H2.2 [Caenorhabditis elegans] ref|NP_492413.1| MeDiaTor family member (mdt-31) [Caenorhabditis elegans] sp|P91869|MED31_CAEEL Probable mediator of RNA polymerase II transcription subunit 31 (Mediator complex subunit 31) (Mediator complex subunit soh-1) 4.0E-75
blast CAE67004 Hypothetical protein CBG12403 [Caenorhabditis briggsae] 6.0E-61
blast CAE67004 Hypothetical protein CBG12403 [Caenorhabditis briggsae] 7.0E-61
blast CAE67004 Hypothetical protein CBG12403 [Caenorhabditis briggsae] 8.0E-60
blast AAH74608 MGC69187 protein [Xenopus tropicalis] ref|NP_001004826.1| MGC69187 protein [Xenopus tropicalis] 1.0E-36
Protein Domains
Database ID Description E-Value
Pfam IPR008831 EMBL-EBI SOH1 5.4E-72
Pfam IPR008831 EMBL-EBI SOH1 5.4E-72
Pfam IPR008831 EMBL-EBI SOH1 5.4E-72
Sequence
Target Sequence 
>C. ELEGANS:190612_AT
aatgtgaattcgtgcaggctcttgcaaatccaaattatcttaattttctcgcgcaacgtg
gttatttcaaagaagaatattttgtcaactatctcaaatatcttctctattggaaagatc
ctcaatatgccagatgcttgaaatttcctcaatgtctgcacatgcttgaagcactgcaga
gtcagcaatttcgcgattcgatggcttacggaccaagcgcgaaattcgtagaagatcaag
tggttctccaatggcagttttatcttcggaaacggcatcgtttgtgtatgatgcctgatg
agggacaagagcttgaagaatctgaagatgaagctgatattcgacaaaaggacacagagg
atgaagatgatgaggaaacgatgaagaaaccggatgctgacacagcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGTGAATTCGTGCAGGCTCTTGC 547 163 53 Antisense
TTATCTTAATTTTCTCGCGCAACGT 17 679 87 Antisense
TTCTCGCGCAACGTGGTTATTTCAA 116 693 98 Antisense
GTCAACTATCTCAAATATCTTCTCT 150 465 136 Antisense
AGATCCTCAATATGCCAGATGCTTG 490 67 168 Antisense
GAAATTTCCTCAATGTCTGCACATG 363 363 192 Antisense
GCAGAGTCAGCAATTTCGCGATTCG 509 311 228 Antisense
TTCGCGATTCGATGGCTTACGGACC 507 607 242 Antisense
GTTCTCCAATGGCAGTTTTATCTTC 227 443 295 Antisense
CGTTTGTGTATGATGCCTGATGAGG 432 247 331 Antisense
GAAGAAACCGGATGCTGACACAGCC 135 339 435 Antisense
Sequence Source Affymetrix Proprietary Database