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[190604_at]

Description T01C8.1 /REP_DB=WormBase Gene ID /WP=CE07458 /TR=Q22068 /GB=AAB00579.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ser-thr-protein kinase
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 38      4grp_highest_8_8 > 56      4grp_high_8_8 > 34      6grp_highest_nonorm > 30      6grp_total > 28      4grp_total > 45      6grp_highest_sub > Cu      6grp_cgc > 06      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 845
1 2 3 4 range
A 1361.9
(3)
P 		1094.1
(-0.1)
P 		850
(-3)
P 		1066
(-0.5)
P 		512
B 1131.7
(-2)
P 		1382.6
(3)
P 		1082
(-3)
P 		1205.1
(-0.5)
P 		301
C 1517
(3)
P 		1137.7
(0.3)
P 		886.1
(-1.5)
P 		671.7
(-3)
P 		845
avr 1336.9
(3) 		1204.8
(1) 		939.4
(-3) 		980.9
(-2.4) 		398

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 850
1 2 3 4 5 6 range
A 1188.6
(3)
P 		946.3
(0.6)
P 		609.2
(-2.6)
P 		572.8
(-3)
P 		982.1
(1)
P 		1072.7
(1.9)
P 		616
B 1260.3
(3)
P 		774.4
(-1.7)
P 		880.2
(-0.7)
P 		774.2
(-1.7)
P 		645.5
(-3)
P 		970.1
(0.2)
P 		615
C 1422.5
(3)
P 		771.8
(-1.8)
P 		832.2
(-1.3)
P 		601.3
(-3)
P 		806.7
(-1.5)
P 		831.3
(-1.3)
P 		821
avr 1290.5
(3) 		830.8
(-1.3) 		773.9
(-1.8) 		649.4
(-3) 		811.4
(-1.5) 		958
(-0.1) 		641
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 389
1 2 3 4 range
A 495.9
(3)
P 		237.5
(-1.6)
P 		222.2
(-1.8)
P 		156
(-3)
P 		340
B 360.9
(3)
P 		341.5
(1.9)
P 		250.7
(-3)
P 		256.8
(-2.7)
P 		110
C 502.2
(3)
P 		327.1
(0.3)
P 		308.8
(0)
P 		113.3
(-3)
P 		389
avr 453
(3) 		302
(-0.3) 		260.6
(-1.2) 		175.4
(-3) 		278

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 406
1 2 3 4 5 6 range
A 219.1
(-0.5)
P 		336.6
(3)
P 		132.7
(-3)
P 		145.3
(-2.6)
P 		296.4
(1.8)
P 		261.2
(0.8)
P 		204
B 438.5
(3)
P 		257.8
(-1.6)
P 		213.7
(-2.8)
P 		205.1
(-3)
P 		262.3
(-1.5)
P 		361.7
(1)
P 		233
C 527.2
(3)
P 		235.5
(-1.3)
P 		211.8
(-1.7)
P 		120.9
(-3)
P 		261
(-0.9)
P 		278.3
(-0.7)
P 		406
avr 394.9
(3) 		276.6
(0) 		186.1
(-2.3) 		157.1
(-3) 		273.2
(-0.1) 		300.4
(0.6) 		238
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190604_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17867
Sequence Type Exemplar sequence
Representative Public ID T01C8.1 NCBI
Target Description T01C8.1 /REP_DB=WormBase Gene ID /WP=CE07458 /TR=Q22068 /GB=AAB00579.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ser-thr-protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078310(11), NM_078309(11), NM_001029697(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078310 NCBI Caenorhabditis elegans AMP-Activated Kinase family member (aak-2) (aak-2) mRNA, complete cds. 11/11 None
NM_078309 NCBI Caenorhabditis elegans AMP-Activated Kinase family member (aak-2) (aak-2) mRNA, complete cds. 11/11 None
NM_001029697 NCBI Caenorhabditis elegans AMP-Activated Kinase family member (aak-2) (aak-2) mRNA, complete cds. 11/11 None
GENEFINDER00000021229 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16796002:16802763:1 11/11 None
SNAP00000021223 ENSEMBL cdna:SNAP chromosome:CEL140:X:16799663:16802763:1 11/11 None
T01C8.1a.2 ENSEMBL cdna:known chromosome:CEL140:X:16795998:16803270:1 gene:T01C8.1 11/11 None
T01C8.1a.1 ENSEMBL cdna:known chromosome:CEL140:X:16796000:16803270:1 gene:T01C8.1 11/11 None
T01C8.1b ENSEMBL cdna:known chromosome:CEL140:X:16796002:16802763:1 gene:T01C8.1 11/11 None
T01C8.1c ENSEMBL cdna:known chromosome:CEL140:X:16799620:16802763:1 gene:T01C8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16799662-16802763(+) 100.0 100.0
Public Domain and Genome References
Ensembl T01C8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR06928 AMP-activated protein kinase alpha subunit 1 [Caenorhabditis elegans] gb|AAM69095.1| Amp-activated kinase protein 2, isoform a [Caenorhabditis elegans] ref|NP_510711.2| AMP-Activated Kinase family member (aak-2) [Caenorhabditis elegans] 0.0
blast AAM69096 Amp-activated kinase protein 2, isoform b [Caenorhabditis elegans] ref|NP_510710.2| AMP-Activated Kinase family member (aak-2) [Caenorhabditis elegans] 0.0
blast AAP13770 Amp-activated kinase protein 2, isoform c [Caenorhabditis elegans] ref|NP_001024868.1| AMP-Activated Kinase family member (aak-2) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-71
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-75
hanks 2.2.7 CaMK Group; CaMK II KIN1/SNF1/Nim1; AKIN10 1.0E-103
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 7.4E-99
Pfam IPR000719 EMBL-EBI Protein kinase 7.4E-99
Sequence
Target Sequence 
>C. ELEGANS:190604_AT
aagggacctggaccagttcatcgccatccagagcgcattgcagcgtcagtcagcagcaag
atcacaccaactctcgacaacacggaagccagtggcgcgaaccgcaacaaacgtgccaag
tggcatctgggtatccgatcccaaagtcgtccggaagacatcatgttcgaggtgttccgt
gcgatgaagcagctggacatggagtggaaggtgctcaacccatatcatgtgattgttcgt
cgcaagcctgatgcacccgctgccgacccgccaaagatgtcgttgcagttgtaccaagtg
gatcagagaagttacttgttggatttcaagagtttggccgacgaggagtctggatccgcc
agtgcatcttcatcccgacacgcatcaatgtctatgccgcagaagccggccggaattcgt
ggaactagaacgtcaagcatgccacaggcgatgagtatggaggcgagtattgagaaaatg
gaagtgcatgatttttcggacatgtcgtgcgatgtgacaccaccaccatctcctggagga
gctaagctttcccagactatgcaattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGGACCTGGACCAGTTCATCGCC 701 121 1093 Antisense
TCATCGCCATCCAGAGCGCATTGCA 4 623 1110 Antisense
CGCATTGCAGCGTCAGTCAGCAGCA 334 253 1126 Antisense
ACCAACTCTCGACAACACGGAAGCC 257 85 1158 Antisense
AAGTGGCATCTGGGTATCCGATCCC 471 159 1210 Antisense
CATCATGTTCGAGGTGTTCCGTGCG 491 213 1251 Antisense
GAAGGTGCTCAACCCATATCATGTG 350 335 1299 Antisense
GATTGTTCGTCGCAAGCCTGATGCA 210 435 1323 Antisense
GCCGACCCGCCAAAGATGTCGTTGC 213 281 1354 Antisense
ATGTCGTGCGATGTGACACCACCAC 393 49 1594 Antisense
TAAGCTTTCCCAGACTATGCAATTC 31 639 1635 Antisense
Sequence Source Affymetrix Proprietary Database