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[190603_s_at] YAAS

Description C05D11.12 /REP_DB=WormBase Gene ID /WP=CE03929 /GEN=let-721 /TR=SW:Q11190 /GB=AAB53835.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=YAAS
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 57      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 78      6grp_total > 58      4grp_total > 47      6grp_highest_sub > Cu      6grp_cgc > 37      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1513
1 2 3 4 range
A 2652.3
(3)
P 		1139.8
(-3)
P 		1389.7
(-2)
P 		1349.6
(-2.2)
P 		1513
B 2430.5
(3)
P 		1245.4
(-3)
P 		1549.4
(-1.5)
P 		1630
(-1.1)
P 		1185
C 2570.6
(3)
P 		1391
(-3)
P 		1616.1
(-1.9)
P 		1618.9
(-1.8)
P 		1180
avr 2551.1
(3) 		1258.7
(-3) 		1518.4
(-1.8) 		1532.8
(-1.7) 		1292

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1355
1 2 3 4 5 6 range
A 1689.8
(-0.1)
P 		1350
(-1.7)
P 		1323.6
(-1.8)
P 		1811.7
(0.4)
P 		1061
(-3)
P 		2376.3
(3)
P 		1315
B 1750.9
(0.5)
P 		1145.7
(-3)
P 		1336.1
(-1.9)
P 		1327.3
(-1.9)
P 		1193.6
(-2.7)
P 		2177.2
(3)
P 		1032
C 1975.2
(0.7)
P 		1265.9
(-3)
P 		1574.4
(-1.4)
P 		1458.6
(-2)
P 		1292.2
(-2.9)
P 		2415.7
(3)
P 		1150
avr 1805.3
(0.3) 		1253.9
(-2.6) 		1411.4
(-1.8) 		1532.5
(-1.2) 		1182.3
(-3) 		2323.1
(3) 		1141
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 768
1 2 3 4 range
A 965.7
(3)
P 		247.4
(-2.6)
P 		363.3
(-1.7)
P 		197.6
(-3)
P 		768
B 775.1
(3)
P 		307.6
(-3)
P 		359
(-2.3)
P 		347.4
(-2.5)
P 		468
C 850.9
(3)
P 		399.9
(-1.7)
P 		563.3
(0)
P 		273
(-3)
P 		578
avr 863.9
(3) 		318.3
(-2.5) 		428.5
(-1.4) 		272.7
(-3) 		591

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 524
1 2 3 4 5 6 range
A 311.5
(-2.5)
P 		480.1
(1)
P 		288.3
(-3)
P 		459.5
(0.5)
P 		320.2
(-2.3)
P 		578.5
(3)
P 		290
B 609.2
(0.5)
P 		381.4
(-2.3)
P 		324.4
(-3)
P 		351.5
(-2.7)
P 		484.9
(-1)
P 		811.9
(3)
P 		488
C 732.1
(2.1)
P 		386.3
(-1.9)
P 		400.7
(-1.7)
P 		293.3
(-3)
P 		418.1
(-1.5)
P 		808.7
(3)
P 		515
avr 550.9
(0.2) 		415.9
(-1.8) 		337.8
(-3) 		368.1
(-2.5) 		407.7
(-1.9) 		733
(3) 		395
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190603_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8002
Sequence Type Exemplar sequence
Representative Public ID C05D11.12 NCBI
Target Description C05D11.12 /REP_DB=WormBase Gene ID /WP=CE03929 /GEN=let-721 /TR=SW:Q11190 /GB=AAB53835.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=YAAS
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066014(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066014 NCBI Caenorhabditis elegans C05D11.12 (YAAS) mRNA, complete cds. 11/11 None
SNAP00000019460 ENSEMBL cdna:SNAP chromosome:CEL140:III:6444814:6446843:-1 11/11 None
GENEFINDER00000019462 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6444814:6446844:-1 11/11 None
C05D11.12.1 ENSEMBL cdna:known chromosome:CEL140:III:6444639:6446844:-1 gene:C05D11.12 11/11 None
C05D11.12.2 ENSEMBL cdna:known chromosome:CEL140:III:6444639:6446880:-1 gene:C05D11.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6444812-6446905(-) 100.0 100.0
Public Domain and Genome References
Gene Title YAAS
Gene Symbol let-721
Ensembl C05D11.12
Entrez Gene 175916 Entrez gene
SwissProt Q11190 EMBL-EBI
WormBase CE29662 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15528_AT electron transfer flavoprotein-ubiquinone oxidoreductase family protein at
ATH1-121501:260536_AT electron transfer flavoprotein-ubiquinone oxidoreductase family protein at
CANINE:1596080_X_AT similar to Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) cfa
CANINE:1595599_AT similar to Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) cfa
CANINE:1592756_X_AT similar to Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) cfa
CANINE:1592755_S_AT similar to Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) cfa
CANINE:1591423_S_AT similar to Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) cfa
CANINE:1583678_AT similar to Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) cfa
CANINE_2:CFA.11262.1.A1_AT similar to Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) cfa
CANINE_2:CFA.11262.1.A1_S_AT similar to Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) cfa
CANINE_2:CFA.265.1.A1_AT similar to Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) cfa
CANINE_2:CFAAFFX.13728.1.S1_AT similar to Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) cfa
DROSOPHILA_2:1628777_AT dm
DROSGENOME1:146911_AT dm
CHICKEN:GGAAFFX.22927.1.S1_AT electron-transferring-flavoprotein dehydrogenase gga
HG-U133_PLUS_2:205530_AT electron-transferring-flavoprotein dehydrogenase hs
HG-U133A_2:205530_AT electron-transferring-flavoprotein dehydrogenase hs
HG-U133A:205530_AT electron-transferring-flavoprotein dehydrogenase hs
HG-FOCUS:205530_AT electron-transferring-flavoprotein dehydrogenase hs
HG-U133A_2:33494_AT electron-transferring-flavoprotein dehydrogenase hs
HG-U133_PLUS_2:33494_AT electron-transferring-flavoprotein dehydrogenase hs
HG-U133A:33494_AT electron-transferring-flavoprotein dehydrogenase hs
HG-U95AV2:33494_AT electron-transferring-flavoprotein dehydrogenase hs
HU35KSUBC:RC_H06935_S_AT electron-transferring-flavoprotein dehydrogenase hs
HUGENEFL:S69232_AT electron-transferring-flavoprotein dehydrogenase hs
U133_X3P:G4758311_3P_S_AT electron-transferring-flavoprotein dehydrogenase hs
HG-U95C:60708_R_AT Electron-transferring-flavoprotein dehydrogenase hs
MG-U74AV2:97869_AT electron transferring flavoprotein, dehydrogenase mm
MOE430A:1451084_AT electron transferring flavoprotein, dehydrogenase mm
MOUSE430A_2:1451084_AT electron transferring flavoprotein, dehydrogenase mm
MOUSE430_2:1451084_AT electron transferring flavoprotein, dehydrogenase mm
MOE430B:1458152_AT electron transferring flavoprotein, dehydrogenase mm
MOUSE430_2:1458152_AT electron transferring flavoprotein, dehydrogenase mm
MU19KSUBA:TC25420_AT Electron transferring flavoprotein, dehydrogenase (Etfdh), mRNA mm
MU19KSUBC:TC37191_AT Electron transferring flavoprotein, dehydrogenase (Etfdh), mRNA mm
RT-U34:RC_AI176422_G_AT electron-transferring-flavoprotein dehydrogenase rn
RG-U34A:RC_AI176422_G_AT electron-transferring-flavoprotein dehydrogenase rn
RT-U34:RC_AI176422_AT electron-transferring-flavoprotein dehydrogenase rn
RG-U34A:RC_AI176422_AT electron-transferring-flavoprotein dehydrogenase rn
RT-U34:RC_AI237007_AT electron-transferring-flavoprotein dehydrogenase rn
RG-U34A:RC_AI237007_AT electron-transferring-flavoprotein dehydrogenase rn
RAE230A:1371519_AT electron-transferring-flavoprotein dehydrogenase rn
RAT230_2:1371519_AT electron-transferring-flavoprotein dehydrogenase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4174 electron-transferring-flavoprotein dehydrogenase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB53835 Lethal protein 721 [Caenorhabditis elegans] ref|NP_498415.2| C05D11.12 [Caenorhabditis elegans] sp|Q11190|ETFD_CAEEL Probable electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring-flavoprotein dehydrogenase) (Lethal protein 721) 0.0
blast D88483 protein let-721 [imported] - Caenorhabditis elegans 0.0
blast CAE72497 Hypothetical protein CBG19676 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007859 EMBL-EBI Electron transfer flavoprotein-ubiquinone oxidoreductase 9.1E-120
Pfam IPR007859 EMBL-EBI Electron transfer flavoprotein-ubiquinone oxidoreductase 9.1E-120
Sequence
Target Sequence 
>C. ELEGANS:190603_S_AT
aacgattgatccagctacatatgacaagaatattcgtgatacttatgttgttaaagagct
caaagcaaccagaaatattcgtccatcattcaatacttcattgggatacattggaggact
gatctattctggaatattctatgttttcggtcgaggaattgaaccatggactcttggaca
cggaaagaaggataatgagaagctcattccagtgaaggatgcaaaagaaatcgattaccc
aaaaccagatggaaagctaacttttgatcttcttacatctgtatctcttactggaaccaa
ccatacagaagatcaacctgctcatttgacattgaaaaatgatcaagttccacttgatgt
gaatttggcagtatatggtggcccggaggctagattctgtccagctggagtctatgaatt
cgtcccatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGATTGATCCAGCTACATATGAC 35 145 1296 Antisense
ATTCGTCCATCATTCAATACTTCAT 457 9 1372 Antisense
AATATTCTATGTTTTCGGTCGAGGA 412 169 1428 Antisense
TGAACCATGGACTCTTGGACACGGA 261 573 1455 Antisense
GAAAGCTAACTTTTGATCTTCTTAC 292 359 1547 Antisense
GATCTTCTTACATCTGTATCTCTTA 616 419 1561 Antisense
GTATCTCTTACTGGAACCAACCATA 266 455 1576 Antisense
GATCAACCTGCTCATTTGACATTGA 10 423 1606 Antisense
GGCAGTATATGGTGGCCCGGAGGCT 669 373 1662 Antisense
CCCGGAGGCTAGATTCTGTCCAGCT 196 261 1677 Antisense
TGGAGTCTATGAATTCGTCCCATCC 637 549 1701 Antisense
Sequence Source Affymetrix Proprietary Database