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[190570_at] acid alpha-glucosidase

Description F52D1.1 /REP_DB=WormBase Gene ID /WP=CE10828 /TR=O17352 /GB=AAB71267.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=acid alpha-glucosidase
Cluster 6grp_highest_10_10 > 17      6grp_high1_10_10 > 61      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 44      6grp_total > 61      4grp_total > 57      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2048
1 2 3 4 range
A 348
(-2.4)
P 		1751.1
(2.5)
P 		1887.5
(3)
P 		192.6
(-3)
P 		1695
B 365.7
(-2.5)
P 		1874.1
(2.2)
P 		2121.9
(3)
P 		188.6
(-3)
P 		1933
C 307.6
(-2.6)
P 		1647.5
(1.3)
P 		2218.2
(3)
P 		170.5
(-3)
P 		2048
avr 340.4
(-2.5) 		1757.6
(2) 		2075.9
(3) 		183.9
(-3) 		1892

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2569
1 2 3 4 5 6 range
A 987.7
(-2)
P 		1633.1
(-0.2)
P 		2350.2
(1.8)
P 		2781.7
(3)
P 		1529.2
(-0.5)
P 		607.7
(-3)
P 		2174
B 1148.1
(-1.3)
P 		1445.1
(-0.4)
P 		2435
(2.5)
P 		2585.7
(3)
P 		1665.5
(0.2)
P 		590.7
(-3)
P 		1995
C 1238.1
(-1.4)
P 		1451.2
(-0.9)
P 		2495.9
(1.5)
P 		3139.8
(3)
P 		1740.1
(-0.3)
P 		570.9
(-3)
P 		2569
avr 1124.6
(-1.6) 		1509.8
(-0.5) 		2427
(1.9) 		2835.7
(3) 		1644.9
(-0.2) 		589.8
(-3) 		2246
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 745
1 2 3 4 range
A 126.7
(-1.7)
P 		380.1
(1.5)
P 		493.4
(3)
P 		28.2
(-3)
P 		465
B 116.6
(-2)
P 		462.9
(2.6)
P 		491.7
(3)
P 		40.2
(-3)
P 		452
C 101.8
(-2.4)
P 		473.7
(0.6)
P 		773.1
(3)
P 		28.7
(-3)
P 		744
avr 115
(-2.1) 		438.9
(1.4) 		586.1
(3) 		32.4
(-3) 		554

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 558
1 2 3 4 5 6 range
A 182.1
(-2.6)
P 		580.8
(1.7)
P 		511.8
(0.9)
P 		705.5
(3)
P 		461.5
(0.4)
P 		148
(-3)
P 		558
B 399.5
(-0.7)
P 		481
(0.4)
P 		591.3
(1.8)
P 		684.9
(3)
P 		676.7
(2.9)
P 		220.3
(-3)
P 		465
C 458.9
(0.6)
P 		442.8
(0.4)
P 		635.2
(3)
P 		631.4
(2.9)
P 		563.1
(2)
P 		191.1
(-3)
P 		444
avr 346.8
(-1) 		501.5
(0.9) 		579.4
(1.8) 		673.9
(3) 		567.1
(1.7) 		186.5
(-3) 		487
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190570_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18451
Sequence Type Exemplar sequence
Representative Public ID F52D1.1 NCBI
Target Description F52D1.1 /REP_DB=WormBase Gene ID /WP=CE10828 /TR=O17352 /GB=AAB71267.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=acid alpha-glucosidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075704(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075704 NCBI Caenorhabditis elegans F52D1.1 (F52D1.1) mRNA, complete cds. 11/11 None
GENEFINDER00000022786 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:460655:466784:1 11/11 None
SNAP00000022778 ENSEMBL cdna:SNAP chromosome:CEL140:X:461927:466784:1 11/11 None
F52D1.1 ENSEMBL cdna:known chromosome:CEL140:X:460461:466913:1 gene:F52D1.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:460460-466784(+) 98.8 98.8
Public Domain and Genome References
Gene Title acid alpha-glucosidase
Ensembl F52D1.1
Entrez Gene 180400 Entrez gene
SwissProt O17352 EMBL-EBI
WormBase CE38522 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.15832.1.S1_S_AT similar to Neutral alpha-glucosidase C cfa
CHICKEN:GGA.16880.1.S1_AT similar to neutral alpha glucosidase C type 2 gga
HG-U133_PLUS_2:235340_AT glucosidase, alpha; neutral C hs
HG-U133B:235340_AT glucosidase, alpha; neutral C hs
HU35KSUBA:RC_AA195050_AT glucosidase, alpha; neutral C hs
U133_X3P:HS.35445.0.A1_3P_AT glucosidase, alpha; neutral C hs
U133_X3P:HS.124437.0.A1_3P_AT glucosidase, alpha; neutral C hs
HG-U133_PLUS_2:236077_AT glucosidase, alpha; neutral C hs
HG-U133B:236077_AT glucosidase, alpha; neutral C hs
HG-U95B:50939_AT glucosidase, alpha; neutral C hs
HG-U95E:81801_AT glucosidase, alpha; neutral C hs
MOUSE430A_2:1436385_AT glucosidase, alpha; neutral C mm
MOE430A:1436385_AT glucosidase, alpha; neutral C mm
MOUSE430_2:1436385_AT glucosidase, alpha; neutral C mm
MG-U74BV2:116839_AT glucosidase, alpha; neutral C mm
MG-U74BV2:111208_AT glucosidase, alpha; neutral C mm
MOE430B:1431063_AT glucosidase, alpha; neutral C mm
MOUSE430_2:1431063_AT glucosidase, alpha; neutral C mm
MG-U74CV2:135093_AT MFLJ00088 protein mm
MG-U74CV2:140457_AT MFLJ00088 protein mm
RG-U34C:RC_AI172595_AT similar to Neutral alpha-glucosidase C rn
RAE230B:1384010_AT similar to Neutral alpha-glucosidase C rn
RAT230_2:1384010_AT similar to Neutral alpha-glucosidase C rn
Protein Similarities
Method ID Description E-Value
blast AAB71267 Hypothetical protein F52D1.1 [Caenorhabditis elegans] ref|NP_508105.2| F52D1.1 [Caenorhabditis elegans] 0.0
blast T32449 hypothetical protein F52D1.1 - Caenorhabditis elegans 0.0
blast CAE63660 Hypothetical protein CBG08162 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.3.1.monodomain cytochrome c All alpha proteins; Cytochrome c; Cytochrome c; monodomain cytochrome c 1.5
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:190570_AT
ggatggttggaaacggtcttctagtgaagccagttcttgaagaaaaggtcaaagagctct
caatctacttgccgggaaaacgtcaagtatggtatgattgggagacccacaaggcacgtc
catcgccaggagcagtccaaattcccgcagagttaaacacaatcggtctttaccacagag
gaggtacaatcattccaaagttgtcagaagtgaaactgactaccaaggagaaccacgagc
aaccaattattctctacattgctgtcaaccagaaaggagatttcgccaatggaacaattt
atttggatgatggagagtcgtatgcttacgagaagggagacttcgcatattggggattca
ctttcaagagagaacacgactacttgcacacaatcaccaacaagaatcttgacaagaagg
gtaaatttgacagtgacgtctacattgacaaaattgtcattcgtggtgccaaattctacc
caagaaatgctcacatcttccttgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATGGTTGGAAACGGTCTTCTAGT 429 513 2219 Antisense
TCTTCTAGTGAAGCCAGTTCTTGAA 53 615 2235 Antisense
CAAAGAGCTCTCAATCTACTTGCCG 359 193 2268 Antisense
GATTGGGAGACCCACAAGGCACGTC 700 433 2314 Antisense
CAGTCCAAATTCCCGCAGAGTTAAA 556 207 2351 Antisense
ACACAATCGGTCTTTACCACAGAGG 357 111 2375 Antisense
GGAGAACCACGAGCAACCAATTATT 353 517 2445 Antisense
TATTCTCTACATTGCTGTCAACCAG 114 665 2466 Antisense
GGATGATGGAGAGTCGTATGCTTAC 554 513 2523 Antisense
GGGAGACTTCGCATATTGGGGATTC 465 495 2553 Antisense
AAGAAATGCTCACATCTTCCTTGAC 356 153 2700 Antisense
Sequence Source Affymetrix Proprietary Database