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[190567_at] serine/threonine kinase

Description K08H2.5 /REP_DB=WormBase Gene ID /WP=CE06163 /TR=Q21373 /GB=CAA94155.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 64      4grp_total > 18      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 4.2
(-3)
A 		77.8
(3)
A 		20
(-1.7)
A 		34.9
(-0.5)
A 		74
B 43.2
(3)
A 		9.1
(-3)
A 		18.3
(-1.4)
A 		40.9
(2.6)
A 		34
C 11.8
(-2.4)
A 		2.8
(-3)
A 		12.3
(-2.3)
A 		86.5
(3)
A 		84
avr 19.7
(-2.5) 		29.9
(-0.9) 		16.9
(-3) 		54.1
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 40.3
(1.2)
A 		47.8
(2.8)
A 		43.3
(1.9)
A 		48.7
(3)
A 		20.3
(-3)
A 		43.4
(1.9)
A 		28
B 9.6
(-2.9)
A 		35.2
(-0.5)
A 		73.2
(3)
A 		44.3
(0.3)
A 		37.5
(-0.3)
A 		8.9
(-3)
A 		64
C 9.3
(-3)
A 		25.8
(-1.1)
A 		9.2
(-3)
A 		9.5
(-3)
A 		12.4
(-2.6)
A 		60.5
(3)
A 		51
avr 19.7
(-3) 		36.3
(1.5) 		41.9
(3) 		34.2
(0.9) 		23.4
(-2) 		37.6
(1.8) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 1.5
(-3)
A 		16.9
(3)
A 		5.2
(-1.6)
A 		5.1
(-1.6)
A 		15
B 13.8
(3)
A 		2.2
(-3)
A 		4.2
(-2)
A 		8.7
(0.4)
A 		12
C 3.9
(-1.7)
A 		0.8
(-3)
A 		4.3
(-1.5)
A 		14.6
(3)
A 		14
avr 6.4
(-0.8) 		6.6
(-0.6) 		4.6
(-3) 		9.5
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 7.4
(-2.3)
A 		17
(3)
A 		9.4
(-1.2)
A 		12.3
(0.4)
A 		6.1
(-3)
A 		10.6
(-0.5)
A 		11
B 3.3
(-3)
A 		11.7
(0.5)
A 		17.8
(3)
A 		11.7
(0.5)
A 		15.2
(1.9)
A 		3.3
(-3)
A 		15
C 3.5
(-2.5)
A 		7.9
(-1)
A 		2.3
(-2.9)
A 		1.9
(-3)
A 		4
(-2.3)
A 		20.3
(3)
A 		18
avr 4.7
(-3) 		12.2
(3) 		9.8
(1.1) 		8.6
(0.1) 		8.4
(-0) 		11.4
(2.4) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190567_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17942
Sequence Type Exemplar sequence
Representative Public ID K08H2.5 NCBI
Target Description K08H2.5 /REP_DB=WormBase Gene ID /WP=CE06163 /TR=Q21373 /GB=CAA94155.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077797(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077797 NCBI Caenorhabditis elegans K08H2.5 (K08H2.5) mRNA, complete cds. 11/11 None
SNAP00000000974 ENSEMBL cdna:SNAP chromosome:CEL140:X:13241029:13242198:-1 11/11 None
GENEFINDER00000000982 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:13241029:13242078:-1 11/11 None
K08H2.5 ENSEMBL cdna:known chromosome:CEL140:X:13241029:13242198:-1 gene:K08H2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13241028-13242198(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase
Ensembl K08H2.5
Entrez Gene 187176 Entrez gene
SwissProt Q21373 EMBL-EBI
WormBase CE06163 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94155 Hypothetical protein K08H2.5 [Caenorhabditis elegans] ref|NP_510198.1| K08H2.5 [Caenorhabditis elegans] 0.0
blast CAA94155 Hypothetical protein K08H2.5 [Caenorhabditis elegans] ref|NP_510198.1| K08H2.5 [Caenorhabditis elegans] 1.0E-175
blast CAA93637 Hypothetical protein C44C10.7 [Caenorhabditis elegans] ref|NP_509953.1| C44C10.7 [Caenorhabditis elegans] 4.0E-72
blast CAB02966 Hypothetical protein F16B12.7 [Caenorhabditis elegans] ref|NP_510350.2| F16B12.7 [Caenorhabditis elegans] 1.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-5
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-7
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-5
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-7
Sequence
Target Sequence 
>C. ELEGANS:190567_AT
tgctttccgttggtaaatctcttcaaactattcattctcttggatatatccatcgtgata
ttcactggaataatgttttatttgccaagacaatcacaccggcttccccatgcaaattga
ttgattatggggttggaaagaaatttcgtaatcgtcgtggaaattacgtgaagaatcgtc
ctcagcgagatgtcaattttaaagaatgcggtcacgtgtcttggaatgtaatgatgggag
gtgtgccgaacttgaaggatgacttttctagtttgatgtttcttggattgaaaatcagtg
gaattagtagtctcgaaattggctctgtcagtgaagtccgccatcagaaaatgatctttg
agcttgacccatctcggtttcttcgtagtgttccatggcttttgaaggtggcttgtgtga
tcgttgacagtgatgcagagaagttcaattacgctggagtctttaacgcaatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTTTCCGTTGGTAAATCTCTTCA 308 575 404 Antisense
CGGCTTCCCCATGCAAATTGATTGA 499 241 503 Antisense
GAATCGTCCTCAGCGAGATGTCAAT 450 329 576 Antisense
AATGCGGTCACGTGTCTTGGAATGT 50 167 608 Antisense
GTAGTCTCGAAATTGGCTCTGTCAG 255 457 710 Antisense
CTCTGTCAGTGAAGTCCGCCATCAG 6 231 726 Antisense
ATGATCTTTGAGCTTGACCCATCTC 362 45 754 Antisense
CCCATCTCGGTTTCTTCGTAGTGTT 354 259 771 Antisense
CTTCGTAGTGTTCCATGGCTTTTGA 533 217 784 Antisense
GAAGGTGGCTTGTGTGATCGTTGAC 25 335 807 Antisense
TACGCTGGAGTCTTTAACGCAATCA 432 649 853 Antisense
Sequence Source Affymetrix Proprietary Database