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[190565_at] serine/threonine kinase

Description C27D8.1 /REP_DB=WormBase Gene ID /WP=CE18539 /TR=Q93288 /GB=CAB02266.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 88      4grp_total > 27      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 5.3
(3)
A 		4.3
(0.1)
A 		5.3
(3)
A 		3.2
(-3)
A 		2
B 2.3
(-3)
A 		6.4
(3)
A 		3.3
(-1.5)
A 		4.5
(0.2)
A 		4
C 3.8
(-1.9)
A 		6.8
(-0.5)
A 		1.6
(-3)
A 		14.1
(3)
A 		13
avr 3.8
(-2.4) 		5.8
(0.7) 		3.4
(-3) 		7.3
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 343
1 2 3 4 5 6 range
A 4
(-3)
A 		19.5
(-2.7)
A 		10.5
(-2.9)
A 		37
(-2.4)
A 		118.4
(-1)
A 		345.2
(3)
P 		341
B 5.1
(-3)
A 		4.1
(-3)
A 		13.1
(-2.8)
A 		30.9
(-2.3)
A 		178.4
(1.5)
P 		237.2
(3)
P 		233
C 8.1
(-2.9)
A 		7.6
(-2.9)
A 		2.5
(-3)
A 		15.3
(-2.8)
A 		164.3
(0.1)
M 		319
(3)
P 		317
avr 5.7
(-3) 		10.4
(-2.9) 		8.7
(-2.9) 		27.7
(-2.6) 		153.7
(0) 		300.5
(3) 		295
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 1.9
(3)
A 		0.9
(-1.3)
A 		1.4
(0.9)
A 		0.5
(-3)
A 		1
B 0.7
(-3)
A 		1.6
(3)
A 		0.8
(-2.3)
A 		1
(-1)
A 		1
C 1.2
(-1)
A 		1.9
(1.3)
A 		0.6
(-3)
A 		2.4
(3)
A 		2
avr 1.3
(1) 		1.5
(3) 		0.9
(-3) 		1.3
(1) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 0.7
(-3)
A 		6.9
(-2.6)
A 		2.3
(-2.9)
A 		9.4
(-2.4)
A 		35.7
(-0.5)
A 		84.1
(3)
P 		83
B 1.8
(-3)
A 		1.4
(-3)
A 		3.2
(-2.9)
A 		8.2
(-2.5)
A 		72.5
(1.9)
P 		88.5
(3)
P 		87
C 3
(-2.9)
A 		2.3
(-2.9)
A 		0.6
(-3)
A 		3.1
(-2.9)
A 		53.2
(-0)
M 		106.8
(3)
P 		106
avr 1.8
(-3) 		3.5
(-2.9) 		2
(-3) 		6.9
(-2.7) 		53.8
(0.4) 		93.1
(3) 		91
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190565_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8930
Sequence Type Exemplar sequence
Representative Public ID C27D8.1 NCBI
Target Description C27D8.1 /REP_DB=WormBase Gene ID /WP=CE18539 /TR=Q93288 /GB=CAB02266.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070027(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070027 NCBI Caenorhabditis elegans C27D8.1 (C27D8.1) mRNA, complete cds. 11/11 None
C27D8.1 ENSEMBL cdna:known chromosome:CEL140:IV:12717859:12719362:1 gene:C27D8.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032214 1/11 Cross Hyb Matching Probes None
GENEFINDER00000032223 1/11 Cross Hyb Matching Probes None
W01G7.2 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12717910-12719328(+) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase
Ensembl C27D8.1
Entrez Gene 178226 Entrez gene
SwissProt Q93288 EMBL-EBI
WormBase CE18539 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02266 Hypothetical protein C27D8.1 [Caenorhabditis elegans] emb|CAB05633.1| Hypothetical protein C27D8.1 [Caenorhabditis elegans] ref|NP_502428.1| C27D8.1 [Caenorhabditis elegans] 0.0
blast CAE59131 Hypothetical protein CBG02430 [Caenorhabditis briggsae] 1.0E-149
blast CAE60406 Hypothetical protein CBG04011 [Caenorhabditis briggsae] 1.0E-148
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-24
Sequence
Target Sequence 
>C. ELEGANS:190565_AT
tactggccttggcgtgtcatgccagtgcttggaggctgtcgaggaacttcataagactgg
gttcattcatcgtgacttaaagcctcagaactatgcatgtgggttggatgagaaacggca
taacatctacattctggactttggtatagctcggaagtacctgaacacgaagaatgagct
gaagacgccgagagaaacagttggatttaagggaacggtccgctttgctccaatcgcctg
ccaccgtaacaccgaaatgggaccgaaggatgactgtgagagctggttctacttacttct
cgatctaatcgtccctagcggtctaccttggcgtaagctctctgacaagcatgaggtgct
caaggagaaggaggaatgccgaaaagataagcgttcctcgttgttcgccgggctcagaca
gactgattatctcagtaaagttttggactacattgacggaagagcttaccaggatcgtgt
ggactaccagttcatctacaagaatcttgcagaggcttgcaaggtttgtaatcttgacat
tgattcaccgtacgattgggagcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACTGGCCTTGGCGTGTCATGCCAG 229 621 381 Antisense
GTCATGCCAGTGCTTGGAGGCTGTC 353 467 396 Antisense
GGCATAACATCTACATTCTGGACTT 283 537 497 Antisense
CCTGCCACCGTAACACCGAAATGGG 196 267 617 Antisense
AGAGCTGGTTCTACTTACTTCTCGA 21 71 659 Antisense
TTACTTCTCGATCTAATCGTCCCTA 325 683 673 Antisense
ACCTTGGCGTAAGCTCTCTGACAAG 316 85 705 Antisense
AAAGATAAGCGTTCCTCGTTGTTCG 657 121 763 Antisense
TTCGCCGGGCTCAGACAGACTGATT 628 695 784 Antisense
GTGTGGACTACCAGTTCATCTACAA 228 483 857 Antisense
GATTCACCGTACGATTGGGAGCTTC 425 429 922 Antisense
Sequence Source Affymetrix Proprietary Database