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[190553_at] triacylglycerol lipase

Description F01G10.7 /REP_DB=WormBase Gene ID /WP=CE09169 /TR=O17766 /GB=CAB02896.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=triacylglycerol lipase
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 61      4grp_highest_8_8 > 53      4grp_high_8_8 > 09      6grp_highest_nonorm > 63      6grp_total > 71      4grp_total > 23      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 449
1 2 3 4 range
A 675.7
(2.9)
P 		399.5
(-2.3)
P 		364
(-3)
P 		679.8
(3)
P 		316
B 739.3
(3)
P 		369.9
(-3)
P 		370.5
(-3)
P 		670.8
(1.9)
P 		369
C 763.3
(3)
P 		347.7
(-2.6)
P 		314.1
(-3)
P 		608.3
(0.9)
P 		449
avr 726.1
(3) 		372.4
(-2.6) 		349.5
(-3) 		653
(1.8) 		377

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 325
1 2 3 4 5 6 range
A 555.6
(-1.5)
P 		528.1
(-2.4)
P 		510.2
(-3)
P 		694.6
(3)
P 		597.9
(-0.1)
P 		548.7
(-1.7)
P 		184
B 502.5
(-1)
P 		474.6
(-1.7)
P 		634
(2.2)
P 		667.6
(3)
P 		573.8
(0.7)
P 		419.6
(-3)
P 		248
C 519.1
(-1.8)
P 		528.5
(-1.6)
P 		744.2
(3)
P 		744.7
(3)
P 		471.3
(-2.9)
P 		464.8
(-3)
P 		280
avr 525.7
(-1.7) 		510.4
(-2.1) 		629.5
(1.1) 		702.3
(3) 		547.7
(-1.1) 		477.7
(-3) 		225
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 246
(3)
P 		86.7
(-3)
P 		95.2
(-2.7)
P 		99.5
(-2.5)
P 		159
B 235.7
(3)
P 		91.3
(-2.8)
P 		85.8
(-3)
P 		143
(-0.7)
P 		150
C 252.7
(3)
P 		100
(-3)
P 		109.5
(-2.6)
P 		102.6
(-2.9)
P 		153
avr 244.8
(3) 		92.7
(-3) 		96.8
(-2.8) 		115
(-2.1) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 102.4
(-3)
P 		187.8
(3)
P 		111.1
(-2.4)
P 		176.2
(2.2)
P 		180.4
(2.5)
P 		133.6
(-0.8)
P 		85
B 174.8
(-1.4)
P 		158
(-2.7)
P 		154
(-3)
P 		176.8
(-1.3)
P 		233.1
(3)
P 		156.5
(-2.8)
P 		79
C 192.4
(3)
P 		161.3
(-1.4)
P 		189.4
(2.6)
P 		149.8
(-3)
P 		152.5
(-2.6)
P 		155.6
(-2.2)
P 		43
avr 156.5
(-1.8) 		169
(0.1) 		151.5
(-2.6) 		167.6
(-0.2) 		188.7
(3) 		148.6
(-3) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190553_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8744
Sequence Type Exemplar sequence
Representative Public ID F01G10.7 NCBI
Target Description F01G10.7 /REP_DB=WormBase Gene ID /WP=CE09169 /TR=O17766 /GB=CAB02896.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=triacylglycerol lipase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069476(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069476 NCBI Caenorhabditis elegans F01G10.7 (F01G10.7) mRNA, complete cds. 11/11 None
SNAP00000013160 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10239809:10241214:-1 10/11 None
GENEFINDER00000013172 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10239295:10241775:-1 10/11 None
F01G10.7 ENSEMBL cdna:known chromosome:CEL140:IV:10239747:10241775:-1 gene:F01G10.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10239752-10241781(-) 100.0 100.0
Public Domain and Genome References
Gene Title triacylglycerol lipase
Ensembl F01G10.7
Entrez Gene 184068 Entrez gene
SwissProt O17766 EMBL-EBI
WormBase CE09169 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
16789 carboxylic ester hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02896 Hypothetical protein F01G10.7 [Caenorhabditis elegans] ref|NP_501877.1| F01G10.7 [Caenorhabditis elegans] 0.0
blast CAE59902 Hypothetical protein CBG03386 [Caenorhabditis briggsae] 0.0
blast CAB02896 Hypothetical protein F01G10.7 [Caenorhabditis elegans] ref|NP_501877.1| F01G10.7 [Caenorhabditis elegans] 1.0E-173
blast CAE59902 Hypothetical protein CBG03386 [Caenorhabditis briggsae] 1.0E-158
blast AAK68537 Hypothetical protein Y57E12B.3 [Caenorhabditis elegans] ref|NP_504755.1| Y57E12B.3 [Caenorhabditis elegans] 3.0E-80
blast AAK68537 Hypothetical protein Y57E12B.3 [Caenorhabditis elegans] ref|NP_504755.1| Y57E12B.3 [Caenorhabditis elegans] 5.0E-77
Protein Domains
Database ID Description E-Value
Pfam IPR000073 EMBL-EBI Alpha/beta hydrolase fold 5.8E-13
Pfam IPR006693 EMBL-EBI Ab-hydrolase associated lipase region 1.5E-42
Pfam IPR000073 EMBL-EBI Alpha/beta hydrolase fold 6.5E-13
Pfam IPR000073 EMBL-EBI Alpha/beta hydrolase fold 1.9E-13
Sequence
Target Sequence 
>C. ELEGANS:190553_AT
actgtcaattccgcgaactgttcaaaaaatgataagttacatgtgcagcagattctttat
gcaaaatatttgtacattggacatcggtttcattgatggaaatgagaaaatgttcaatca
atcaagagttggtgtatatctttgtcatacacctgcagccacttctgtaaaagatttgca
acactggattcaacttgtcaaatcacagactgtttctaagtttgattatggaactgatgg
aaatatcattgaatacgggcaacctactcctccggaatatgatttgacacagatcaacac
accaacttatctctattggagtcgtgacgacattttggctgatactcaagatattcgaga
ctcgattctgtcaaaaatgaataaaacaatcgctggatcactggaacttccacactacag
tcatatggattttgtattcggtacgcatgctgcatttgatttatatccggtaagtctgaa
atcaaatttcctactgaataatatcttttcattaacgttagaccgcgcatctgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTGTCAATTCCGCGAACTGTTCAA 621 97 696 Antisense
GTACATTGGACATCGGTTTCATTGA 171 461 767 Antisense
TATATCTTTGTCATACACCTGCAGC 446 661 830 Antisense
CACCTGCAGCCACTTCTGTAAAAGA 115 201 845 Antisense
CAACCTACTCCTCCGGAATATGATT 187 185 955 Antisense
ATCAACACACCAACTTATCTCTATT 414 27 988 Antisense
GTCGTGACGACATTTTGGCTGATAC 174 473 1016 Antisense
ACAATCGCTGGATCACTGGAACTTC 60 115 1081 Antisense
GGAACTTCCACACTACAGTCATATG 552 527 1098 Antisense
GTATTCGGTACGCATGCTGCATTTG 516 451 1129 Antisense
ATTAACGTTAGACCGCGCATCTGTA 298 13 1206 Antisense
Sequence Source Affymetrix Proprietary Database