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[190546_s_at]

Description T25F10.6 /REP_DB=WormBase Gene ID /WP=CE07537 /TR=Q23050 /GB=AAB04989.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=calponin-like protein
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 41      4grp_highest_8_8 > 08      4grp_high_8_8 > 53      6grp_highest_nonorm > 07      6grp_total > 52      4grp_total > 59      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20071
1 2 3 4 range
A 10558.8
(-0.1)
P 		20700.4
(3)
P 		18534
(2.3)
P 		752.1
(-3)
P 		19948
B 10243.3
(0.1)
P 		17521.2
(2.4)
P 		19253.7
(3)
P 		629.7
(-3)
P 		18624
C 11058.4
(0.7)
P 		17425.3
(3)
P 		16325.3
(2.6)
P 		653.9
(-3)
P 		16771
avr 10620.2
(0.3) 		18549
(3) 		18037.7
(2.8) 		678.6
(-3) 		17870

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9376
1 2 3 4 5 6 range
A 12323.3
(0.4)
P 		14626.8
(2.5)
P 		15250.4
(3)
P 		12885.9
(0.9)
P 		11916.2
(0.1)
P 		8380.8
(-3)
P 		6870
B 11217.4
(-1)
P 		13341.7
(0.5)
P 		16837.4
(3)
P 		12688.4
(0)
P 		11389.6
(-0.9)
P 		8464.8
(-3)
P 		8373
C 11531.4
(-0.6)
P 		13188.3
(0.5)
P 		17107.1
(3)
P 		13193
(0.5)
P 		13449
(0.7)
P 		7731.3
(-3)
P 		9376
avr 11690.7
(-0.4) 		13718.9
(1) 		16398.3
(3) 		12922.4
(0.5) 		12251.6
(-0) 		8192.3
(-3) 		8206
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5580
1 2 3 4 range
A 3844.5
(1.7)
P 		4493.5
(2.6)
P 		4845.1
(3)
P 		110.1
(-3)
P 		4735
B 3266.5
(1.3)
P 		4327.3
(2.8)
P 		4461.5
(3)
P 		134.2
(-3)
P 		4327
C 3660.6
(0.8)
P 		5009.9
(2.3)
P 		5689.9
(3)
P 		110.3
(-3)
P 		5580
avr 3590.5
(1.3) 		4610.2
(2.5) 		4998.8
(3) 		118.2
(-3) 		4881

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3162
1 2 3 4 5 6 range
A 2271.7
(-2.6)
P 		5202
(3)
P 		3321.2
(-0.6)
P 		3268.2
(-0.7)
P 		3595.8
(-0)
P 		2040.5
(-3)
P 		3162
B 3903.1
(0)
P 		4440.9
(2.2)
P 		4088.6
(0.8)
P 		3360.7
(-2.2)
P 		4627.5
(3)
P 		3156.5
(-3)
P 		1471
C 4274.2
(2.7)
P 		4024.1
(1.9)
P 		4354.1
(3)
P 		2653.1
(-2.8)
P 		4351.9
(3)
P 		2588.1
(-3)
P 		1766
avr 3483
(-0.3) 		4555.7
(3) 		3921.3
(1.1) 		3094
(-1.5) 		4191.7
(1.9) 		2595
(-3) 		1961
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190546_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11947
Sequence Type Exemplar sequence
Representative Public ID T25F10.6 NCBI
Target Description T25F10.6 /REP_DB=WormBase Gene ID /WP=CE07537 /TR=Q23050 /GB=AAB04989.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=calponin-like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028988(9), NM_001028987(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028988 NCBI Caenorhabditis elegans T25F10.6b (T25F10.6) mRNA, complete cds. 9/11 None
NM_001028987 NCBI Caenorhabditis elegans T25F10.6a (T25F10.6) mRNA, complete cds. 11/11 None
SNAP00000017681 ENSEMBL cdna:SNAP chromosome:CEL140:V:6768580:6771046:-1 11/11 None
GENEFINDER00000017688 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6768580:6771046:-1 11/11 None
T25F10.6b.1 ENSEMBL cdna:known chromosome:CEL140:V:6768271:6771928:-1 gene:T25F10.6 9/11 None
T25F10.6b.2 ENSEMBL cdna:known chromosome:CEL140:V:6768386:6771928:-1 gene:T25F10.6 9/11 None
T25F10.6a ENSEMBL cdna:known chromosome:CEL140:V:6768397:6771115:-1 gene:T25F10.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6768580-6771047(-) 100.0 100.0
Public Domain and Genome References
Ensembl T25F10.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB04989 Hypothetical protein T25F10.6a [Caenorhabditis elegans] ref|NP_001024158.1| T25F10.6a [Caenorhabditis elegans] 0.0
blast CAE71947 Hypothetical protein CBG19014 [Caenorhabditis briggsae] 0.0
blast AAT81185 Hypothetical protein T25F10.6b [Caenorhabditis elegans] ref|NP_001024159.1| T25F10.6b [Caenorhabditis elegans] 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR000557 EMBL-EBI Calponin repeat 3.6E-10
Pfam IPR000557 EMBL-EBI Calponin repeat 5.3E-5
Pfam IPR000557 EMBL-EBI Calponin repeat 0.0080
Pfam IPR000557 EMBL-EBI Calponin repeat 4.8E-7
Pfam IPR000557 EMBL-EBI Calponin repeat 0.022
Pfam IPR000557 EMBL-EBI Calponin repeat 1.2E-6
Pfam IPR000557 EMBL-EBI Calponin repeat 5.1E-9
Pfam IPR000557 EMBL-EBI Calponin repeat 1.2E-13
Pfam IPR000557 EMBL-EBI Calponin repeat 0.0080
Pfam IPR000557 EMBL-EBI Calponin repeat 4.8E-7
Pfam IPR000557 EMBL-EBI Calponin repeat 0.022
Sequence
Target Sequence 
>C. ELEGANS:190546_S_AT
gctcgcatctcaagctggaatgaccggaatcggaatgccacgtattgttgatgtgagaag
gaccaacgaccaggatcgtgactcgcaaggattcattcaccttcagatgggaaccaacaa
gtttgccaaccaagctggtatgaccggattcggtatgccacgtcacaacatcaccaagta
caaggacgaggttcgcggagacatgccacacgacgaaggaaccctttcaagacagacctc
tggatggaaggagggagcttcgcaagccggtatgaccggattcggagccttccgtaacaa
caccgtcgccttcatgcaggctcaagaccaacgatcacagggaatgattccataccagat
gggagtcaacttcctcgaatcacaagccggaaagaccggattcggacagccaagacaggt
gtacactccattcactgacgatactcacgaggatctgccagctgatatcgcccgccgacc
ggatgttcctttctggacaggacagaaggaggctgatcatgctaaccagaccggtatgac
tgccttcggtac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCGCATCTCAAGCTGGAATGACC 508 295 639 Antisense
GACCAGGATCGTGACTCGCAAGGAT 158 383 706 Antisense
TGACCGGATTCGGTATGCCACGTCA 142 571 779 Antisense
GACATGCCACACGACGAAGGAACCC 69 369 838 Antisense
GGTATGACCGGATTCGGAGCCTTCC 373 503 907 Antisense
TGCAGGCTCAAGACCAACGATCACA 709 577 953 Antisense
ATGGGAGTCAACTTCCTCGAATCAC 52 51 997 Antisense
GACAGGTGTACACTCCATTCACTGA 420 369 1052 Antisense
CGAGGATCTGCCAGCTGATATCGCC 448 249 1086 Antisense
GGCTGATCATGCTAACCAGACCGGT 147 539 1149 Antisense
AGACCGGTATGACTGCCTTCGGTAC 99 71 1166 Antisense
Sequence Source Affymetrix Proprietary Database