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[190545_at]

Description C04G6.1 /REP_DB=WormBase Gene ID /WP=CE06762 /TR=Q17642 /GB=AAA98016.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Ser-Thr protein kinase
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 81      4grp_highest_8_8 > 42      4grp_high_8_8 > 40      6grp_highest_nonorm > 81      6grp_total > 90      4grp_total > 55      6grp_highest_sub > 04_05      6grp_cgc > 96      6grp_high1_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 470
1 2 3 4 range
A 490.1
(3)
P 		146.5
(-2.1)
P 		197
(-1.4)
P 		86.2
(-3)
P 		404
B 498.9
(3)
P 		150.3
(-2.1)
P 		185.3
(-1.6)
P 		85.4
(-3)
P 		414
C 535.3
(3)
P 		123.6
(-2.3)
P 		211.2
(-1.1)
P 		65.2
(-3)
P 		470
avr 508.1
(3) 		140.1
(-2.1) 		197.8
(-1.3) 		78.9
(-3) 		429

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 158
1 2 3 4 5 6 range
A 192.6
(-2.4)
P 		223.4
(-1.3)
P 		178.1
(-3)
P 		335.6
(3)
P 		239.3
(-0.7)
P 		194.1
(-2.4)
P 		158
B 281.2
(1.6)
P 		270.9
(1.1)
P 		198.4
(-3)
P 		305.7
(3)
P 		281.8
(1.7)
P 		229
(-1.3)
P 		107
C 251.5
(0.1)
P 		222.2
(-3)
P 		233.6
(-1.8)
P 		278.5
(3)
P 		253.2
(0.3)
P 		251.5
(0.1)
P 		56
avr 241.8
(-0.8) 		238.8
(-0.9) 		203.4
(-3) 		306.6
(3) 		258.1
(0.2) 		224.9
(-1.8) 		103
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 178.4
(3)
P 		31.8
(-2.3)
P 		51.5
(-1.6)
P 		12.6
(-3)
P 		166
B 159.1
(3)
P 		37.1
(-2.2)
P 		42.9
(-1.9)
P 		18.2
(-3)
P 		141
C 177.2
(3)
P 		35.5
(-2.1)
P 		73.6
(-0.7)
P 		11
(-3)
P 		166
avr 171.6
(3) 		34.8
(-2.2) 		56
(-1.4) 		13.9
(-3) 		158

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 35.5
(-3)
P 		79.4
(2.3)
P 		38.8
(-2.6)
P 		85.1
(3)
P 		72.2
(1.4)
P 		47.3
(-1.6)
P 		50
B 97.8
(1.5)
P 		90.2
(0.8)
P 		48.2
(-3)
P 		81
(-0)
P 		114.5
(3)
P 		85.4
(0.4)
P 		66
C 93.2
(3)
P 		67.8
(-1.1)
P 		59.5
(-2.4)
P 		56
(-3)
P 		81.9
(1.2)
P 		84.2
(1.5)
P 		37
avr 75.5
(0.9) 		79.1
(1.5) 		48.8
(-3) 		74
(0.7) 		89.5
(3) 		72.3
(0.5) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190545_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5127
Sequence Type Exemplar sequence
Representative Public ID C04G6.1 NCBI
Target Description C04G6.1 /REP_DB=WormBase Gene ID /WP=CE06762 /TR=Q17642 /GB=AAA98016.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Ser-Thr protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062545(11), NM_062546(11), NM_001026742(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062545 NCBI Caenorhabditis elegans MAP Kinase family member (mpk-2) (mpk-2) mRNA, complete cds. 11/11 A 
NM_062546 NCBI Caenorhabditis elegans MAP Kinase family member (mpk-2) (mpk-2) mRNA, complete cds. 11/11 None
NM_001026742 NCBI Caenorhabditis elegans MAP Kinase family member (mpk-2) (mpk-2) mRNA, complete cds. 11/11 None
SNAP00000008315 ENSEMBL cdna:SNAP chromosome:CEL140:II:5104628:5106974:1 11/11 None
C04G6.1c ENSEMBL cdna:known chromosome:CEL140:II:5104100:5108791:1 gene:C04G6.1 11/11 A 
C04G6.1a ENSEMBL cdna:known chromosome:CEL140:II:5104446:5108854:1 gene:C04G6.1 11/11 A 
C04G6.1b ENSEMBL cdna:known chromosome:CEL140:II:5104446:5107016:1 gene:C04G6.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5104443-5106487(+) 100.0 100.0
Public Domain and Genome References
Ensembl C04G6.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA98016 Map kinase protein 2, isoform a [Caenorhabditis elegans] ref|NP_494946.2| MAP Kinase family member (mpk-2) [Caenorhabditis elegans] 0.0
blast AAT81209 Map kinase protein 2, isoform c [Caenorhabditis elegans] ref|NP_001021913.1| MAP Kinase family member (mpk-2) [Caenorhabditis elegans] 0.0
blast CAE59045 Hypothetical protein CBG02327 [Caenorhabditis briggsae] 0.0
blast AAN60533 Map kinase protein 2, isoform b [Caenorhabditis elegans] ref|NP_494947.2| MAP Kinase family member (mpk-2) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-72
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-72
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-73
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-73
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 4.0E-75
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 2.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-86
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-86
Sequence
Target Sequence 
>C. ELEGANS:190545_AT
gagaatcgccgattttggcatggcaagagcttatgcttctgcgagtacagtaagagatga
cgctaatgttggaggacatatgacacaatatgtatcaacacgttggtatcgagctccaga
gattttgttttcaatggtagaatatgacacaaaagtggatttgtggtcagctggatgtat
atttgctgaaatgctacttcgacgtcagctattccctggaaaagattccgtttcacagat
aaaaatgattgtttattatttgggaagtccagaagaagaggttatcaataggataacatc
agaccttgtcagagattcaattgaagcatgtggtcgtaaaactcctcttccattttctgc
aatatttccgaaagctagtccagaggcgagaaacatggtttcatatctattgcaaatttc
tccctggaaacgatattcggcagatcagattctacaacatccgttcatggctcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAATCGCCGATTTTGGCATGGCA 21 391 600 Antisense
AGAGCTTATGCTTCTGCGAGTACAG 449 69 625 Antisense
AACACGTTGGTATCGAGCTCCAGAG 73 137 696 Antisense
GTGGATTTGTGGTCAGCTGGATGTA 695 489 754 Antisense
GAAATGCTACTTCGACGTCAGCTAT 226 361 787 Antisense
CGACGTCAGCTATTCCCTGGAAAAG 364 251 799 Antisense
GCATGTGGTCGTAAAACTCCTCTTC 97 311 925 Antisense
TCCTCTTCCATTTTCTGCAATATTT 416 593 942 Antisense
TTCCGAAAGCTAGTCCAGAGGCGAG 683 697 965 Antisense
TTCTCCCTGGAAACGATATTCGGCA 96 689 1017 Antisense
TCTACAACATCCGTTCATGGCTCAA 539 613 1050 Antisense
Sequence Source Affymetrix Proprietary Database