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[190534_at] alcohol dehydrogenase

Description R05D8.10 /REP_DB=WormBase Gene ID /WP=CE21092 /TR=Q9N5G1 /GB=AAF39943.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=alcohol dehydrogenase
Cluster 6grp_total > 56      4grp_total > 22      6grp_cgc > 37      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 310
1 2 3 4 range
A 317.7
(3)
A 		209.4
(0.9)
A 		13.3
(-3)
A 		271.7
(2.1)
A 		304
B 287.8
(2.2)
A 		42.5
(-3)
A 		116.4
(-1.4)
A 		323
(3)
P 		281
C 264.3
(1.8)
A 		52.9
(-2.5)
A 		29
(-3)
A 		320.2
(3)
A 		291
avr 289.9
(2.6) 		101.6
(-1.8) 		52.9
(-3) 		305
(3) 		252

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 221
1 2 3 4 5 6 range
A 78.5
(-0.9)
A 		120.3
(0.3)
A 		151.6
(1.2)
A 		152.9
(1.2)
A 		6.9
(-3)
A 		214.2
(3)
A 		207
B 159
(-0.4)
A 		136.2
(-1.5)
A 		115.8
(-2.5)
A 		105.2
(-3)
A 		131.8
(-1.7)
A 		228.3
(3)
A 		123
C 139.4
(2)
A 		161.8
(3)
A 		78.3
(-0.8)
A 		34.3
(-2.8)
A 		29.2
(-3)
A 		140.2
(2)
A 		133
avr 125.6
(0) 		139.4
(0.6) 		115.2
(-0.4) 		97.5
(-1.2) 		56
(-3) 		194.2
(3) 		138
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 115.7
(3)
A 		45.4
(-0.8)
A 		3.5
(-3)
A 		39.8
(-1.1)
A 		112
B 91.8
(3)
A 		10.5
(-3)
A 		27
(-1.8)
A 		68.8
(1.3)
P 		81
C 87.5
(3)
A 		15.2
(-2.6)
A 		10.1
(-3)
A 		54
(0.4)
A 		77
avr 98.3
(3) 		23.7
(-2.3) 		13.5
(-3) 		54.2
(-0.1) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 14.5
(-1.5)
A 		42.8
(1.9)
A 		33
(0.7)
A 		38.8
(1.4)
A 		2.1
(-3)
A 		52.1
(3)
A 		50
B 55.3
(-0.1)
A 		45.3
(-1.2)
A 		28.1
(-3)
A 		27.9
(-3)
A 		53.5
(-0.3)
A 		85.2
(3)
A 		57
C 51.7
(3)
A 		49.4
(2.7)
A 		19.9
(-1.3)
A 		6.9
(-3)
A 		9.4
(-2.7)
A 		46.9
(2.4)
A 		45
avr 40.5
(-0.2) 		45.8
(0.6) 		27
(-2.2) 		24.5
(-2.6) 		21.7
(-3) 		61.4
(3) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190534_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15771
Sequence Type Exemplar sequence
Representative Public ID R05D8.10 NCBI
Target Description R05D8.10 /REP_DB=WormBase Gene ID /WP=CE21092 /TR=Q9N5G1 /GB=AAF39943.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=alcohol dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071353(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071353 NCBI Caenorhabditis elegans DeHydrogenases, Short chain family member (dhs-15) (dhs-15) mRNA, complete cds. 11/11 None
SNAP00000009123 ENSEMBL cdna:SNAP chromosome:CEL140:V:2766098:2769580:-1 11/11 None
GENEFINDER00000009146 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2768246:2769580:-1 11/11 None
R05D8.10 ENSEMBL cdna:known chromosome:CEL140:V:2768246:2769602:-1 gene:R05D8.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2768245-2769580(-) 100.0 100.0
Public Domain and Genome References
Gene Title alcohol dehydrogenase
Gene Symbol dhs-15
Ensembl R05D8.10
Entrez Gene 178739 Entrez gene
SwissProt Q9N5G1 EMBL-EBI
WormBase CE21092 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAE62263 Hypothetical protein CBG06322 [Caenorhabditis briggsae] 0.0
blast NP_503754 DeHydrogenases, Short chain family member (dhs-15) [Caenorhabditis elegans] gb|AAF39943.1| Dehydrogenases, short chain protein 15 [Caenorhabditis elegans] 1.0E-146
blast NP_503754 DeHydrogenases, Short chain family member (dhs-15) [Caenorhabditis elegans] gb|AAF39943.1| Dehydrogenases, short chain protein 15 [Caenorhabditis elegans] 1.0E-141
blast NP_503753 R05D8.9 [Caenorhabditis elegans] gb|AAF39944.1| Hypothetical protein R05D8.9 [Caenorhabditis elegans] 1.0E-135
blast CAE62263 Hypothetical protein CBG06322 [Caenorhabditis briggsae] 1.0E-114
blast NP_503751 R05D8.7 [Caenorhabditis elegans] gb|AAF39950.1| Hypothetical protein R05D8.7 [Caenorhabditis elegans] pdb|1XKQ|D Chain D, Crystal Structure Of Short-Chain DehydrogenaseREDUCTASE OF Unknown Function From Caenorhabditis Elegans With Cofactor pdb|1XKQ|C Chain C, Crystal Structure Of Short-Chain DehydrogenaseREDUCTASE OF Unknown Function From Caenorhabditis Elegans With Cofactor pdb|1XKQ|B Chain B, Crystal Structure Of Short-Chain DehydrogenaseREDUCTASE OF Unknown Function From Caenorhabditis Elegans With Cofactor pdb|1XKQ|A Chain A, Crystal Structure Of Short-Chain DehydrogenaseREDUCTASE OF Unknown Function From Caenorhabditis Elegans With Cofactor 2.0E-77
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 3.0E-59
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 5.7E-50
Sequence
Target Sequence 
>C. ELEGANS:190534_AT
caatgctggagcggcgtttgctgacccatctggaaaaattggctccgaggcggcgattgg
catttttgatgatatgatgaagttgaacttgagaagtgttgtggagcttgtcaaaaagtg
tcgtccgcacttaatcgctagcaaaggggaaatcgttaatgtttcttcaattgctgctgg
tccacaaccgtacatctactacacttactacggaatctgcaaggccggcctggaccagct
caccaggagcttagccttggagctgattcccttcgatgttcgtgtcaactccgtgagccc
tggactcatatcaaccaacttcctgggcgctgtaggtatgggagatgatgtggtgaaaaa
gacggaggagtactactccactcaccgagattgtattccggctggccgtactggaaaacc
tgaagaaatcgcatcacttatcgcgtttttggctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATGCTGGAGCGGCGTTTGCTGAC 675 181 288 Antisense
AAATTGGCTCCGAGGCGGCGATTGG 560 115 323 Antisense
AAAGTGTCGTCCGCACTTAATCGCT 268 121 402 Antisense
TTTCTTCAATTGCTGCTGGTCCACA 149 673 449 Antisense
CAACCGTACATCTACTACACTTACT 287 187 472 Antisense
GCTCACCAGGAGCTTAGCCTTGGAG 315 301 525 Antisense
TCCGTGAGCCCTGGACTCATATCAA 168 595 577 Antisense
TCATATCAACCAACTTCCTGGGCGC 439 619 593 Antisense
TACTACTCCACTCACCGAGATTGTA 679 643 658 Antisense
GATTGTATTCCGGCTGGCCGTACTG 296 435 676 Antisense
GCATCACTTATCGCGTTTTTGGCTG 40 309 718 Antisense
Sequence Source Affymetrix Proprietary Database