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[190533_s_at] alanyl-tRNA synthetase

Description W02B12.6 /REP_DB=WormBase Gene ID /WP=CE28096 /TR=Q23122 /GB=CAA91396.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=alanyl-tRNA synthetase
Cluster 6grp_high1_10_10 > 43      4grp_high_8_8 > 48      6grp_total > 20      4grp_total > 55      6grp_cgc > 72      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 139.6
(3)
P 		94.2
(-0.4)
A 		94.1
(-0.4)
P 		58.5
(-3)
A 		81
B 157.4
(3)
P 		105.3
(-0.2)
P 		65
(-2.6)
M 		58.6
(-3)
P 		99
C 118.5
(2.4)
P 		88.3
(-3)
P 		119.3
(2.6)
P 		121.6
(3)
P 		33
avr 138.5
(3) 		95.9
(-1.3) 		92.8
(-1.7) 		79.6
(-3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 147
1 2 3 4 5 6 range
A 94.3
(0.4)
P 		130.5
(2.8)
P 		133.1
(3)
P 		119.5
(2.1)
P 		85.1
(-0.3)
A 		45
(-3)
A 		88
B 113.4
(-1.6)
M 		146
(1)
P 		170.2
(3)
A 		111.2
(-1.8)
P 		96.8
(-3)
P 		129.2
(-0.4)
P 		73
C 75.3
(-2.1)
P 		192.1
(3)
M 		102.8
(-0.9)
P 		99.9
(-1)
A 		53.9
(-3)
A 		84
(-1.7)
P 		138
avr 94.3
(-1.8) 		156.2
(3) 		135.4
(1.4) 		110.2
(-0.6) 		78.6
(-3) 		86.1
(-2.4) 		78
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 50.8
(3)
P 		20.5
(-1.3)
A 		24.6
(-0.7)
P 		8.6
(-3)
A 		42
B 50.2
(3)
P 		26
(-0.9)
P 		15.1
(-2.6)
M 		12.5
(-3)
P 		38
C 39.2
(2.3)
P 		25.4
(-1.6)
P 		41.6
(3)
P 		20.5
(-3)
P 		21
avr 46.7
(3) 		24
(-1.2) 		27.1
(-0.6) 		13.9
(-3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 17.4
(-1.9)
P 		46.4
(3)
P 		29
(0.1)
P 		30.3
(0.3)
P 		25.7
(-0.5)
A 		10.9
(-3)
A 		36
B 39.4
(0.1)
M 		48.6
(3)
P 		41.3
(0.7)
A 		29.4
(-3)
P 		39.3
(0.1)
P 		48.2
(2.9)
P 		19
C 27.9
(-1.5)
P 		58.6
(3)
M 		26.2
(-1.7)
P 		20.1
(-2.6)
A 		17.4
(-3)
A 		28.1
(-1.4)
P 		41
avr 28.2
(-2.6) 		51.2
(3) 		32.2
(-1.6) 		26.6
(-3) 		27.5
(-2.8) 		29.1
(-2.4) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190533_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4017
Sequence Type Exemplar sequence
Representative Public ID W02B12.6 NCBI
Target Description W02B12.6 /REP_DB=WormBase Gene ID /WP=CE28096 /TR=Q23122 /GB=CAA91396.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=alanyl-tRNA synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064043(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064043 NCBI Caenorhabditis elegans Alanyl tRNA Synthetase family member (ars-1) (ars-1) mRNA, complete cds. 11/11 A 
SNAP00000002910 ENSEMBL cdna:SNAP chromosome:CEL140:II:11460235:11463926:-1 11/11 None
GENEFINDER00000002921 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11460235:11463926:-1 11/11 None
W02B12.6 ENSEMBL cdna:known chromosome:CEL140:II:11460137:11463981:-1 gene:W02B12.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11460231-11463923(-) 100.0 100.0
Public Domain and Genome References
Gene Title alanyl-tRNA synthetase
Gene Symbol ars-1
Ensembl W02B12.6
Entrez Gene 174749 Entrez gene
SwissProt Q23122 EMBL-EBI
WormBase CE28096 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6419 alanyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4813 alanine-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91396 Hypothetical protein W02B12.6 [Caenorhabditis elegans] ref|NP_496444.1| Alanyl tRNA Synthetase family member (ars-1) [Caenorhabditis elegans] gb|AAF05590.1| alanyl-tRNA synthetase [Caenorhabditis elegans] 0.0
blast CAE59502 Hypothetical protein CBG02888 [Caenorhabditis briggsae] 0.0
blast CAE66735 Hypothetical protein CBG12085 [Caenorhabditis briggsae] 1.0E-152
Protein Domains
Database ID Description E-Value
Pfam IPR002318 EMBL-EBI Alanyl-tRNA synthetase, class IIc 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:190533_S_AT
gcgtgatgagagctcactcggcgacccatttgctcaattgggctctgcaaaaactcggtg
taggctctgggcagaaaggctcatctgttgactgcgatcgcttcagatttgactattcca
cgggagacgaagatttgagcaaggagcaaagaacggaacttttgattaaatgtgaaatga
aaatgagggaattcattcaaaatggaggtttcacagaaattatagagacgagtttggaag
aagcaaagaaaatcgaaaatctgcaatctgatgtgaaagaggatagaattggaggcgctt
cagtgagagttgtggctttaggcagtggagctgatgtgccagtggagtgctgttctggaa
cacacatccatgacgtcagagtgattgatgacgtggcaattatgtcggacaagtcaatgg
gtcaaaggcttcgaagaattatagtcctcactggaaaggaagcagctgcgtgcagaaact
atacaaaaagcatttatgaagatttaaggagtacggatccaaaggagagatcaaaaacgg
gaaagaacatcgactggaaacgtgtcccaattgctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGTGATGAGAGCTCACTCGGCGAC 287 291 1904 Antisense
GCGACCCATTTGCTCAATTGGGCTC 482 289 1924 Antisense
CAAAAACTCGGTGTAGGCTCTGGGC 98 189 1951 Antisense
AAAGGCTCATCTGTTGACTGCGATC 511 121 1978 Antisense
GACTGCGATCGCTTCAGATTTGACT 289 375 1993 Antisense
CAGATTTGACTATTCCACGGGAGAC 633 203 2007 Antisense
AATTGGAGGCGCTTCAGTGAGAGTT 114 179 2190 Antisense
GAGAGTTGTGGCTTTAGGCAGTGGA 431 395 2208 Antisense
GATGTGCCAGTGGAGTGCTGTTCTG 404 409 2236 Antisense
GGCTTCGAAGAATTATAGTCCTCAC 526 537 2330 Antisense
GACTGGAAACGTGTCCCAATTGCTG 613 375 2455 Antisense
Sequence Source Affymetrix Proprietary Database