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[190525_s_at] Glutamine synthetase

Description K03H1.1 /REP_DB=WormBase Gene ID /WP=CE00507 /TR=SW:P34497 /GB=CAA82655.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Glutamine synthetase
Cluster 6grp_total > 98      4grp_total > 12      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 100.8
(-0.7)
A 		69.5
(-3)
A 		73.6
(-2.7)
A 		150
(3)
A 		81
B 68.6
(0.7)
A 		65.6
(0.4)
A 		24.6
(-3)
A 		96.5
(3)
A 		72
C 37.8
(-2.9)
A 		51.2
(-1.9)
A 		35.8
(-3)
A 		123.2
(3)
M 		87
avr 69.1
(-1.1) 		62.1
(-1.7) 		44.7
(-3) 		123.2
(3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 456
1 2 3 4 5 6 range
A 63.9
(-2.6)
A 		43.2
(-3)
A 		50.5
(-2.9)
A 		116.1
(-1.7)
A 		375.6
(3)
P 		348
(2.5)
P 		332
B 79
(-2.7)
A 		59.4
(-3)
A 		61
(-3)
A 		125.2
(-2.1)
A 		282.9
(0)
P 		499.6
(3)
P 		440
C 69.7
(-3)
A 		75.3
(-2.9)
A 		72.3
(-3)
A 		137.9
(-2)
A 		350.3
(1.2)
P 		470.6
(3)
P 		401
avr 70.9
(-2.8) 		59.3
(-3) 		61.3
(-3) 		126.4
(-1.9) 		336.3
(1.4) 		439.4
(3) 		380
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 36.7
(3)
A 		15.1
(-3)
A 		19.2
(-1.9)
A 		22
(-1.1)
A 		22
B 21.9
(3)
A 		16.2
(0.9)
A 		5.7
(-3)
A 		20.6
(2.5)
A 		16
C 12.5
(-3)
A 		14.7
(-1.4)
A 		12.5
(-3)
A 		20.8
(3)
M 		8
avr 23.7
(3) 		15.3
(-1.5) 		12.5
(-3) 		21.1
(1.6) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 175
1 2 3 4 5 6 range
A 11.8
(-3)
A 		15.4
(-2.7)
A 		11
(-3)
A 		29.5
(-1.9)
A 		113.3
(3)
P 		84.7
(1.3)
P 		102
B 27.5
(-2.6)
A 		19.8
(-2.8)
A 		14.8
(-3)
A 		33.2
(-2.4)
A 		114.9
(0.5)
P 		186.3
(3)
P 		172
C 25.8
(-2.7)
A 		23
(-2.8)
A 		18.4
(-3)
A 		27.7
(-2.6)
A 		113.3
(1.1)
P 		157.6
(3)
P 		139
avr 21.7
(-2.7) 		19.4
(-2.8) 		14.7
(-3) 		30.1
(-2.3) 		113.8
(1.6) 		142.9
(3) 		128
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190525_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6132
Sequence Type Exemplar sequence
Representative Public ID K03H1.1 NCBI
Target Description K03H1.1 /REP_DB=WormBase Gene ID /WP=CE00507 /TR=SW:P34497 /GB=CAA82655.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Glutamine synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066807(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066807 NCBI Caenorhabditis elegans GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-2) (gln-2) mRNA, complete cds. 11/11 None
SNAP00000019346 ENSEMBL cdna:SNAP chromosome:CEL140:III:9943208:9944569:-1 11/11 None
GENEFINDER00000019360 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9943208:9944569:-1 11/11 None
K03H1.1 ENSEMBL cdna:known chromosome:CEL140:III:9943144:9944653:-1 gene:K03H1.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021251 1/11 Cross Hyb Matching Probes None
GENEFINDER00000021264 1/11 Cross Hyb Matching Probes None
F59A7.10 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9943203-9944565(-) 100.0 100.0
Public Domain and Genome References
Gene Title Glutamine synthetase
Gene Symbol gln-2
Ensembl K03H1.1
Entrez Gene 176407 Entrez gene
SwissProt P34497 EMBL-EBI
WormBase CE00507 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256524_AT glutamine synthetase, putative at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9399 nitrogen fixation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4356 glutamate-ammonia ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA82655 Hypothetical protein K03H1.1 [Caenorhabditis elegans] ref|NP_499208.1| GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-2) [Caenorhabditis elegans] sp|P34497|GLNA_CAEEL Probable glutamine synthetase (Glutamate--ammonia ligase) 0.0
blast CAE73310 Hypothetical protein CBG20737 [Caenorhabditis briggsae] 0.0
blast CAE73232 Hypothetical protein CBG20640 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 5.1E-34
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 1.1E-98
Sequence
Target Sequence 
>C. ELEGANS:190525_S_AT
tagcgtaggagctgatcgtgcgttcgggagagaagtcgtcgagacacattaccgggcttg
tcttcacgccggactcaacatttttggaacaaatgccgaagtgactccaggacaatggga
attccaaattggaacctgcgaaggaatcgatatgggagatcagttgtggatgtcgagata
cattctgcacagagttgctgaacaattcggtgtctgcgtatcccttgatcccaaaccaaa
ggtcaccatgggagactggaacggagccggatgccacaccaacttctcgactgccgaaat
gcgtgctccaggtggaattgctgcgattgaagccgccatgacaggactcaagcggacaca
tttggaggcgatgaaggtgtacgatccacatggtggagaagacaatcttcgtcgtttgac
aggacgtcatgagacaagttcggctgacaaattctcatggggagtcgccaatcgtggatg
ctcaatccgtattccgagacaggtggctgcggagagaaaaggatatctggaggatcgtcg
tccgtcatcaaactgcgatccttatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAGCGTAGGAGCTGATCGTGCGTTC 553 649 513 Antisense
AGTCGTCGAGACACATTACCGGGCT 536 1 546 Antisense
AATGCCGAAGTGACTCCAGGACAAT 259 167 604 Antisense
ACCAACTTCTCGACTGCCGAAATGC 37 85 790 Antisense
GATTGAAGCCGCCATGACAGGACTC 128 435 837 Antisense
GAAGACAATCTTCGTCGTTTGACAG 86 341 910 Antisense
GTTCGGCTGACAAATTCTCATGGGG 701 441 950 Antisense
GGAGTCGCCAATCGTGGATGCTCAA 475 517 973 Antisense
GGATGCTCAATCCGTATTCCGAGAC 26 515 988 Antisense
TATCTGGAGGATCGTCGTCCGTCAT 627 657 1036 Antisense
CCGTCATCAAACTGCGATCCTTATC 645 263 1054 Antisense
Sequence Source Affymetrix Proprietary Database