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[190511_at]

Description T27E9.4 /REP_DB=WormBase Gene ID /WP=CE14269 /CHR=3 /FEA=Sanger Annotation /DEF=ring canal protein like (HINXTON) TR:O18147 protein_id:CAB04878.1
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 72      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 65      6grp_total > 72      4grp_total > 22      6grp_highest_sub > 03_04      6grp_cgc > 75      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 420
1 2 3 4 range
A 1023.8
(1.7)
P 		899.1
(-0.6)
P 		769.7
(-3)
P 		1093.4
(3)
P 		324
B 1163.2
(3)
P 		967.5
(-1.3)
P 		888.1
(-3)
P 		975.6
(-1.1)
P 		275
C 1073.7
(0.5)
P 		924.9
(-2.6)
P 		907.2
(-3)
P 		1190
(3)
P 		283
avr 1086.9
(3) 		930.5
(-1) 		855
(-3) 		1086.3
(3) 		232

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 956
1 2 3 4 5 6 range
A 640.3
(-3)
P 		1160.6
(0.3)
P 		1332.8
(1.3)
P 		1596.1
(3)
P 		1044.4
(-0.5)
P 		1063.9
(-0.3)
P 		956
B 832
(-3)
P 		1059.4
(0.2)
P 		1199
(2.1)
P 		1260.1
(3)
P 		1200
(2.2)
P 		948.7
(-1.4)
P 		428
C 1006.1
(-1.6)
P 		992.3
(-1.8)
P 		1298.2
(3)
P 		1245.8
(2.2)
P 		918.4
(-3)
P 		941.8
(-2.6)
P 		380
avr 826.1
(-3) 		1070.8
(-0.3) 		1276.7
(2) 		1367.3
(3) 		1054.3
(-0.5) 		984.8
(-1.2) 		541
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 213
1 2 3 4 range
A 372.8
(3)
P 		195.2
(-2)
P 		201.2
(-1.8)
P 		160.1
(-3)
P 		213
B 370.9
(3)
P 		239
(-1.8)
P 		205.8
(-3)
P 		207.9
(-2.9)
P 		165
C 355.4
(3)
P 		265.9
(-0.5)
P 		316.2
(1.5)
P 		200.6
(-3)
P 		155
avr 366.4
(3) 		233.4
(-1.5) 		241.1
(-1.2) 		189.5
(-3) 		177

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 370
1 2 3 4 5 6 range
A 118
(-3)
P 		412.7
(3)
P 		290.2
(0.5)
P 		404.8
(2.8)
P 		315.2
(1)
P 		259
(-0.1)
P 		295
B 289.5
(-3)
P 		352.6
(-1.1)
P 		291.2
(-2.9)
P 		333.8
(-1.7)
P 		487.5
(3)
P 		353.8
(-1.1)
P 		198
C 372.9
(3)
P 		302.8
(-0.4)
P 		330.4
(0.9)
P 		250.5
(-3)
P 		297.2
(-0.7)
P 		315.3
(0.2)
P 		122
avr 260.1
(-3) 		356
(2.4) 		303.9
(-0.5) 		329.7
(0.9) 		366.6
(3) 		309.4
(-0.2) 		107
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190511_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6745
Sequence Type Exemplar sequence
Representative Public ID T27E9.4 NCBI
Target Description T27E9.4 /REP_DB=WormBase Gene ID /WP=CE14269 /CHR=3 /FEA=Sanger Annotation /DEF=ring canal protein like (HINXTON) TR:O18147 protein_id:CAB04878.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067384(11), NM_067383(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067384 NCBI Caenorhabditis elegans KELch-repeat containing protein family member (kel-3) (kel-3) mRNA, complete cds. 11/11 None
NM_067383 NCBI Caenorhabditis elegans KELch-repeat containing protein family member (kel-3) (kel-3) mRNA, complete cds. 11/11 None
SNAP00000014266 ENSEMBL cdna:SNAP chromosome:CEL140:III:13466095:13474117:1 11/11 None
GENEFINDER00000014275 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13466095:13470823:1 11/11 None
T27E9.4a.2 ENSEMBL cdna:known chromosome:CEL140:III:13464345:13470850:1 gene:T27E9.4 11/11 None
T27E9.4a.1 ENSEMBL cdna:known chromosome:CEL140:III:13466092:13470850:1 gene:T27E9.4 11/11 None
T27E9.4b ENSEMBL cdna:known chromosome:CEL140:III:13466094:13470823:1 gene:T27E9.4 11/11 None
T27E9.4a.3 ENSEMBL cdna:known chromosome:CEL140:III:13466094:13470850:1 gene:T27E9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13465898-13470819(+) 100.0 100.0
Public Domain and Genome References
Ensembl T27E9.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04878 Hypothetical protein T27E9.4a [Caenorhabditis elegans] ref|NP_499785.2| KELch-repeat containing protein family member (kel-3) [Caenorhabditis elegans] 0.0
blast CAC42351 Hypothetical protein T27E9.4b [Caenorhabditis elegans] ref|NP_499784.2| KELch-repeat containing protein family member (kel-3) [Caenorhabditis elegans] 0.0
blast CAE73692 Hypothetical protein CBG21203 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006652 EMBL-EBI Kelch repeat 9.7E-8
Pfam IPR006652 EMBL-EBI Kelch repeat 1.1E-11
Pfam IPR006652 EMBL-EBI Kelch repeat 8.2E-14
Pfam IPR006652 EMBL-EBI Kelch repeat 2.7E-16
Pfam IPR006652 EMBL-EBI Kelch repeat 1.2E-19
Pfam IPR006652 EMBL-EBI Kelch repeat 2.7E-9
Pfam IPR000210 EMBL-EBI BTB/POZ domain 2.2E-40
Pfam IPR006652 EMBL-EBI Kelch repeat 9.7E-8
Pfam IPR006652 EMBL-EBI Kelch repeat 1.1E-11
Pfam IPR006652 EMBL-EBI Kelch repeat 8.2E-14
Pfam IPR006652 EMBL-EBI Kelch repeat 2.7E-16
Pfam IPR006652 EMBL-EBI Kelch repeat 1.2E-19
Pfam IPR006652 EMBL-EBI Kelch repeat 2.7E-9
Pfam IPR000210 EMBL-EBI BTB/POZ domain 1.5E-38
Pfam IPR004277 EMBL-EBI Phosphatidyl serine synthase 1.9E-80
Pfam IPR006652 EMBL-EBI Kelch repeat 7.9E-10
Pfam IPR006652 EMBL-EBI Kelch repeat 8.2E-14
Pfam IPR006652 EMBL-EBI Kelch repeat 2.7E-16
Pfam IPR006652 EMBL-EBI Kelch repeat 1.2E-19
Pfam IPR006652 EMBL-EBI Kelch repeat 2.7E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000014266 3 657-674,687-709,713-735
Sequence
Target Sequence 
>C. ELEGANS:190511_AT
caagaaaacgttctgctctggcggctgcattcgtcacaaatcgtttatatgtgtgtggtg
gatatgatggtctacatagtctttcttcaattgaaatttacgatattaatcgaaatgtct
gggaagccggaccaccaatggaaaatatgagaagtgccgctggagttactgttattgata
aacatatttatatttgtggtggccacgatggaatgcaaatcttcgcatcagtcgaacgat
tggacacagaaaatcaacaatgggaacgaattccttcaatgattcagcaaagatgccgat
ttggagctgccacgttcaaaggaaaaatctacgtggctggtggatacgatggaacttcat
tccttaaatctgttgaagtttatgatccagttgagaagaaatggtctccagtgtcaccga
tgaatatgagaagaagccgggtgtcactcgtatcaactaacgagggattattcgcagttg
caggattcgatggcgagaacaacttgtgcagtatggaacaatacgatgacgtcacagatt
catggaccattgcaactgctctcacttgtcatgaaggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGAAAACGTTCTGCTCTGGCGGC 42 183 1268 Antisense
GCTGCATTCGTCACAAATCGTTTAT 324 297 1291 Antisense
GATGGTCTACATAGTCTTTCTTCAA 37 409 1333 Antisense
GAAATGTCTGGGAAGCCGGACCACC 318 359 1379 Antisense
GAGAAGTGCCGCTGGAGTTACTGTT 551 389 1416 Antisense
GATTTGGAGCTGCCACGTTCAAAGG 163 427 1565 Antisense
GGAACTTCATTCCTTAAATCTGTTG 211 527 1618 Antisense
ATGGTCTCCAGTGTCACCGATGAAT 430 51 1668 Antisense
GAAGAAGCCGGGTGTCACTCGTATC 137 339 1697 Antisense
TTCATGGACCATTGCAACTGCTCTC 456 687 1806 Antisense
AACTGCTCTCACTTGTCATGAAGGT 641 141 1821 Antisense
Sequence Source Affymetrix Proprietary Database